| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 972,121 | T→A | 7.9% | L137Q (CTG→CAG) | secD → | protein translocase subunit SecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 972,121 | 0 | T | A | 7.9% | 198.1 / 7.1 | 63 | L137Q (CTG→CAG) | secD | protein translocase subunit SecD |
| Reads supporting (aligned to +/- strand): ref base T (27/31); new base A (2/3); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
GCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGATGC > NC_002947/971972‑972265 | gccagccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCctgct < 2:507945/150‑1 (MQ=255) ccagccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCctgctg > 1:406488/1‑150 (MQ=255) aaGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg > 2:61571/1‑150 (MQ=255) aGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgg < 2:814319/150‑1 (MQ=255) gATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggac < 2:604268/150‑1 (MQ=255) tGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacat < 2:406488/150‑1 (MQ=255) gTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatg < 2:1057412/150‑1 (MQ=255) tAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatgg < 1:331691/150‑1 (MQ=255) tGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcacaccaccg < 2:1422611/150‑7 (MQ=255) tGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcacaccaccg < 2:1422626/150‑7 (MQ=255) cACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGa < 2:932440/150‑1 (MQ=255) gatTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacat > 2:1405890/1‑125 (MQ=255) gatTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacat < 1:1405890/125‑1 (MQ=255) tcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg < 1:414114/113‑1 (MQ=255) tcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg > 2:414114/1‑113 (MQ=255) tcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa > 2:1323743/1‑146 (MQ=255) tcgtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 1:1323743/146‑1 (MQ=255) gtGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTAc < 2:714430/150‑1 (MQ=255) ccTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAgg > 1:1295770/1‑150 (MQ=255) tGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCg > 2:71223/1‑150 (MQ=255) aaCCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAg < 2:1295770/150‑1 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAg > 2:694737/1‑150 (MQ=255) cccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGTCCTGGACCTGTCCGGTGGTATGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGa < 1:804590/150‑1 (MQ=255) cccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg < 1:844253/97‑1 (MQ=255) cccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg > 2:844253/1‑97 (MQ=255) cccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTgcg > 2:698497/1‑150 (MQ=255) ccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg < 1:582357/96‑1 (MQ=255) ccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg > 2:582357/1‑96 (MQ=255) aaCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa > 2:1063775/1‑66 (MQ=255) aaCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa < 1:1063775/66‑1 (MQ=255) aaCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTtg > 2:53329/1‑129 (MQ=255) aaCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTtg < 1:53329/129‑1 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga < 2:1078577/68‑1 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga > 1:1078577/1‑68 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGa > 1:452537/1‑85 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGa < 2:452537/85‑1 (MQ=255) tGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAgc > 2:951759/1‑138 (MQ=255) tGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAgc < 1:951759/138‑1 (MQ=255) gcAAGCCCGATGAAACGGGGCCTCGACCTGCCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgc < 1:559590/150‑1 (MQ=255) cAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGGCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcc > 1:5362/1‑150 (MQ=255) gATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTca > 1:997486/1‑150 (MQ=255) tGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTcagc > 2:1120837/1‑150 (MQ=255) aCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGa < 1:694737/150‑1 (MQ=255) gggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCCCAGCatgatgc > 1:460727/1‑149 (MQ=255) ggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATggc < 1:71223/150‑1 (MQ=255) aCCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGcatca > 1:1091825/1‑150 (MQ=255) aCCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGcatca > 1:899666/1‑150 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatggac > 1:1417876/1‑42 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggacatggac < 2:1417876/42‑1 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATggcgg > 1:423903/1‑143 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATggcgg < 2:423903/143‑1 (MQ=255) tGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCATGATGCCGCCATCCTGc < 1:555308/150‑1 (MQ=255) gTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCt < 2:1268414/150‑1 (MQ=255) tCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTAc > 1:207108/1‑114 (MQ=255) tCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTAc < 2:207108/114‑1 (MQ=255) cGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCCCAGCATGATGCCGCCATCctgctgag < 2:460727/150‑3 (MQ=255) gtgtGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCg < 2:1192076/150‑1 (MQ=255) tgtgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGc < 1:698497/150‑1 (MQ=255) gtgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTcc < 1:836563/100‑1 (MQ=255) gtgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTcc > 2:836563/1‑100 (MQ=255) gtccACTTCCAGCAGGAAGTGCACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTcagca > 1:360915/4‑122 (MQ=255) gtccACTTCCAGCAGGAAGTGCACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTcagca < 2:360915/119‑1 (MQ=255) tccACTTCCAGCAGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTg > 2:463060/3‑150 (MQ=255) cACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGGCTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAc > 1:1343263/1‑150 (MQ=255) ccTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGATGc > 1:854569/1‑150 (MQ=255) tgctgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTAc < 1:676015/50‑1 (MQ=255) tgctgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTAc > 2:676015/1‑50 (MQ=255) | GCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGATGC > NC_002947/971972‑972265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |