Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 986,238 C→G 9.3% A78G (GCC→GGC)  PP_0852 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947986,2380CG 9.3% 162.0 / 8.1 54A78G (GCC→GGC) PP_0852Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (31/18);  new base G (3/2);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

TCTCAAGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTG  >  NC_002947/986093‑986387
                                                                                                                                                  |                                                                                                                                                     
tctcAAGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGGCGCG‑AGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGgccgcc                                                                                                                                                   <  1:260490/150‑1 (MQ=255)
  tcAAGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCt                                                                                                                                                 >  2:740151/1‑150 (MQ=255)
  tcAAGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCt                                                                                                                                                 >  1:558693/1‑150 (MQ=255)
    aaGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTgg                                                                                                                                               <  2:680919/150‑1 (MQ=255)
          aGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATg                                                                                                                                         <  2:558693/150‑1 (MQ=255)
               gCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAACCCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTc                                                                                                                                    <  1:796319/150‑1 (MQ=255)
               gCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTc                                                                                                                                    >  1:960878/1‑150 (MQ=255)
                cccGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCAGCCCTGGTAGATGCCTTCg                                                                                                                                   >  2:938329/1‑150 (MQ=255)
                 ccGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCACCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGa                                                                                                                                  >  1:666687/1‑150 (MQ=255)
                            aCCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                          <  1:1165481/131‑1 (MQ=255)
                            aCCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                          >  2:1165481/1‑131 (MQ=255)
                              cTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGt                                                                                                                     >  2:933011/1‑150 (MQ=255)
                                 aCTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                          >  1:948804/1‑126 (MQ=255)
                                 aCTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                          <  2:948804/126‑1 (MQ=255)
                                     tCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACg                                                                                                              >  2:1200183/1‑150 (MQ=255)
                                     tCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACg                                                                                                              >  2:580930/1‑150 (MQ=255)
                                        gTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGGCGGCCTGGTCGa                                                                                                                                           <  1:375744/118‑1 (MQ=255)
                                        gTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGGCGGCCTGGTCGa                                                                                                                                           >  2:375744/1‑118 (MQ=255)
                                                  ccACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGa                                                                                                 >  1:838257/1‑150 (MQ=255)
                                                   cACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGAc                                                                                                >  1:752247/1‑150 (MQ=255)
                                                   cACGTGGAAACCGGCGCCTTCCACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGAc                                                                                                <  2:943868/150‑1 (MQ=255)
                                                    aCGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTG‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCATTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACg                                                                                               >  1:374097/1‑150 (MQ=255)
                                                     cGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGAACAGGCCGCCCTCATACACATCTTCTACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGt                                                                                              <  1:740151/150‑1 (MQ=255)
                                                        ggAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTg                                                                                                                                                >  2:10244/1‑97 (MQ=255)
                                                        ggAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTg                                                                                                                                                <  1:10244/97‑1 (MQ=255)
                                                        ggAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCa                                                                                           >  1:1036599/1‑150 (MQ=255)
                                                              cGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCt                                                                                     <  2:752247/150‑1 (MQ=255)
                                                               ggCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                    >  1:345152/1‑150 (MQ=255)
                                                                    cTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCg                                                                               >  1:1160985/1‑150 (MQ=255)
                                                                     ttCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGt                                                                              >  1:1145503/1‑150 (MQ=255)
                                                                      tCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTa                                                                             <  1:933011/150‑1 (MQ=255)
                                                                       cGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTAt                                                                            <  2:1068449/150‑1 (MQ=255)
                                                                        gACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                    >  1:1355030/1‑141 (MQ=255)
                                                                        gACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                    <  2:1355030/141‑1 (MQ=255)
                                                                               ttcccGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGc                                                                    >  1:1274100/3‑150 (MQ=255)
                                                                                    gggCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCtgtg                                                               >  2:118453/1‑150 (MQ=255)
                                                                                      gCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCg                                                             <  1:909936/150‑1 (MQ=255)
                                                                                        aTACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGt                                                           >  1:1073869/1‑150 (MQ=255)
                                                                                            tttC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTc                                                       >  1:988286/1‑150 (MQ=255)
                                                                                             ttC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCg                                                      >  1:610318/1‑150 (MQ=255)
                                                                                                     gTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAata                                               <  2:960878/150‑1 (MQ=255)
                                                                                                        gCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCATGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATcc                                            >  1:1244038/1‑150 (MQ=255)
                                                                                                             aTTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTgcgc                                       >  1:322609/1‑150 (MQ=255)
                                                                                                              ttCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGcgcg                                      >  2:1075292/1‑150 (MQ=255)
                                                                                                                                tGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGaa                                                                        >  2:1308767/1‑98 (MQ=255)
                                                                                                                                tGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGaa                                                                        <  1:1308767/98‑1 (MQ=255)
                                                                                                                                 gATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCt                   <  2:610318/150‑1 (MQ=255)
                                                                                                                                     gACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAAGATCCTGCACGGCGTCCGCCTGCTGTTGCtggtt               <  2:838257/150‑2 (MQ=255)
                                                                                                                                       ccTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGc             >  2:797872/1‑150 (MQ=255)
                                                                                                                                        atctaCCAGGGCGGCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCt            >  2:1056493/5‑150 (MQ=255)
                                                                                                                                           gACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtgtg         >  1:18594/1‑150 (MQ=255)
                                                                                                                                            aCCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtg           <  2:1017933/147‑1 (MQ=255)
                                                                                                                                            aCCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtg           >  1:1017933/1‑147 (MQ=255)
                                                                                                                                             ccAGGGCGGCCTGGTCGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGc                                                                                 >  1:987345/1‑76 (MQ=255)
                                                                                                                                             ccAGGGCGGCCTGGTCGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGc                                                                                 <  2:987345/76‑1 (MQ=255)
                                                                                                                                                  ccgccCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCggtg  <  2:988286/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TCTCAAGCCGAGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTC‑GCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTG  >  NC_002947/986093‑986387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑