Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 986,248 A→C 7.5% V81V (GTA→GTC)  PP_0852 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947986,2480AC 7.5% 149.2 / 3.4 53V81V (GTA→GTC) PP_0852Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (31/18);  new base C (2/2);  total (33/20)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTGGTGGCT  >  NC_002947/986103‑986393
                                                                                                                                                 |                                                                                                                                                 
aGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATg                                                                                                                                               <  2:558693/150‑1 (MQ=255)
     gCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAACCCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTc                                                                                                                                          <  1:796319/150‑1 (MQ=255)
     gCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTc                                                                                                                                          >  1:960878/1‑150 (MQ=255)
      cccGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCAGCCCTGGTAGATGCCTTCg                                                                                                                                         >  2:938329/1‑150 (MQ=255)
       ccGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCACCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGa                                                                                                                                        >  1:666687/1‑150 (MQ=255)
                  aCCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                                >  2:1165481/1‑131 (MQ=255)
                  aCCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                                <  1:1165481/131‑1 (MQ=255)
                    cTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGt                                                                                                                           >  2:933011/1‑150 (MQ=255)
                       aCTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                                <  2:948804/126‑1 (MQ=255)
                       aCTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGAt                                                                                                                                                >  1:948804/1‑126 (MQ=255)
                           tCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACg                                                                                                                    >  2:580930/1‑150 (MQ=255)
                           tCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACg                                                                                                                    >  2:1200183/1‑150 (MQ=255)
                              gTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGGCGGCCTGGTCGa                                                                                                                                                 >  2:375744/1‑118 (MQ=255)
                              gTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGGCGGCCTGGTCGa                                                                                                                                                 <  1:375744/118‑1 (MQ=255)
                                        ccACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGa                                                                                                       >  1:838257/1‑150 (MQ=255)
                                         cACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGAc                                                                                                      >  1:752247/1‑150 (MQ=255)
                                         cACGTGGAAACCGGCGCCTTCCACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGAc                                                                                                      <  2:943868/150‑1 (MQ=255)
                                          aCGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTGGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCATTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACg                                                                                                     >  1:374097/1‑150 (MQ=255)
                                           cGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGAACAGGCCGCCCTCATACACATCTTCTACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGt                                                                                                    <  1:740151/150‑1 (MQ=255)
                                              ggAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCa                                                                                                 >  1:1036599/1‑150 (MQ=255)
                                                    cGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCt                                                                                           <  2:752247/150‑1 (MQ=255)
                                                     ggCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                          >  1:345152/1‑150 (MQ=255)
                                                          cTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCg                                                                                     >  1:1160985/1‑150 (MQ=255)
                                                           ttCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGt                                                                                    >  1:1145503/1‑150 (MQ=255)
                                                            tCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTa                                                                                   <  1:933011/150‑1 (MQ=255)
                                                             cGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTAt                                                                                  <  2:1068449/150‑1 (MQ=255)
                                                              gACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                          >  1:1355030/1‑141 (MQ=255)
                                                              gACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTg                                                                                          <  2:1355030/141‑1 (MQ=255)
                                                                     ttcccGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGc                                                                          >  1:1274100/3‑150 (MQ=255)
                                                                          gggCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCtgtg                                                                     >  2:118453/1‑150 (MQ=255)
                                                                            gCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCg                                                                   <  1:909936/150‑1 (MQ=255)
                                                                              aTACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGt                                                                 >  1:1073869/1‑150 (MQ=255)
                                                                                  tttCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTc                                                             >  1:988286/1‑150 (MQ=255)
                                                                                   ttCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCg                                                            >  1:610318/1‑150 (MQ=255)
                                                                                          gTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAata                                                     <  2:960878/150‑1 (MQ=255)
                                                                                             gCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCATGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATcc                                                  >  1:1244038/1‑150 (MQ=255)
                                                                                                  aTTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTgcgc                                             >  1:322609/1‑150 (MQ=255)
                                                                                                   ttCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGcgcg                                            >  2:1075292/1‑150 (MQ=255)
                                                                                                                     tGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGaa                                                                              <  1:1308767/98‑1 (MQ=255)
                                                                                                                     tGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGaa                                                                              >  2:1308767/1‑98 (MQ=255)
                                                                                                                      gATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCt                         <  2:610318/150‑1 (MQ=255)
                                                                                                                          gACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAAGATCCTGCACGGCGTCCGCCTGCTGTTGCtggtt                     <  2:838257/150‑2 (MQ=255)
                                                                                                                            ccTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGc                   >  2:797872/1‑150 (MQ=255)
                                                                                                                             atctaCCAGGGCGGCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCt                  >  2:1056493/5‑150 (MQ=255)
                                                                                                                                gACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtgtg               >  1:18594/1‑150 (MQ=255)
                                                                                                                                 aCCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtg                 <  2:1017933/147‑1 (MQ=255)
                                                                                                                                 aCCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCtg                 >  1:1017933/1‑147 (MQ=255)
                                                                                                                                  ccAGGGCGGCCTGGTCGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGc                                                                                       >  1:987345/1‑76 (MQ=255)
                                                                                                                                  ccAGGGCGGCCTGGTCGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGc                                                                                       <  2:987345/76‑1 (MQ=255)
                                                                                                                                       ccgccCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCggtg        <  2:988286/150‑1 (MQ=255)
                                                                                                                                           ccTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGtggtgg    >  2:555844/1‑150 (MQ=255)
                                                                                                                                            cTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTGGTGGc   <  2:1145503/150‑1 (MQ=255)
                                                                                                                                             tGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTGGTGGCt  >  2:35858/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGGTGGCCCGTAAGCTCAACCTCACTGTCAGTTCGTTGAACCACGTGGAAACCGGCGCCTTCGACAAGCTGCCCGGGCATACTTTCGCTCGTGGCTACATTCGCGCCTACGCCAAGCTGATGGACCTCGACCAGGCCGCCCTGGTAGATGCCTTCGACCGCTACACCGGTACCCACGCCAAAGGCAGCGACGTGCATTCGCTGGGCCGTATCGAAGAGCCTGTGCGGTTGTCGCACAATATCCTGCGCGGCGTCAGCCTGCTGTTGCTGGTGGCTGTGGTCGGTGGTGGCT  >  NC_002947/986103‑986393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑