Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 87,493 T→A 13.8% N98I (AAT→ATT)  PP_0076 ← choline betaine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294787,4930TA13.8% 130.7 / 11.2 50N98I (AAT→ATT) PP_0076choline betaine‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/18);  new base A (3/4);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACA  >  NC_002947/87353‑87640
                                                                                                                                            |                                                                                                                                                   
cGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCTAGGGTCAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGATgt                                                                                                                                            <  2:621749/150‑2 (MQ=255)
 gTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGATgtg                                                                                                                                           <  2:1394249/150‑3 (MQ=255)
  tACAGTTTTTTTTTTTTCTTCCCTGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAAc                                                                                                                                          >  2:868603/1‑150 (MQ=255)
    cAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACtt                                                                                                                                        <  2:941059/150‑1 (MQ=255)
                gAACTGCCCGGCGTGTTTTTTCAGGTTAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGcc                                                                                                                            >  2:1245082/1‑150 (MQ=255)
                   cTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtg                                                                                                                         <  1:1306262/150‑1 (MQ=255)
                    tGCCCTGCGTTTTTTTGCAGGTCAGCGAAGTCCTTCCCCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtgc                                                                                                                        <  2:198455/150‑1 (MQ=255)
                     gCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCCCCAATCGTGTGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtgct                                                                                                                       >  1:811961/1‑150 (MQ=255)
                          gCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGcc                                                                                                                  <  1:868603/150‑1 (MQ=255)
                             tgtTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGAt                                                                                                                                              >  2:818832/1‑119 (MQ=255)
                             tgtTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGAt                                                                                                                                              <  1:818832/119‑1 (MQ=255)
                              gtTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTgg                                                                                                                                                  <  2:839437/114‑1 (MQ=255)
                              gtTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTgg                                                                                                                                                  >  1:839437/1‑114 (MQ=255)
                                        gTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGTGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGACCTTGTCATGGAAGCCTTGCTGCACCGGCATCCAGTTTcg                                                                                                    >  2:303434/1‑149 (MQ=255)
                                            gCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAgg                                                                                                >  1:1133889/1‑150 (MQ=255)
                                                 gTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGc                                                                                           >  1:843843/1‑150 (MQ=255)
                                                                 ttCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTcc                                                                           >  2:646284/1‑150 (MQ=255)
                                                                     acacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGAGCTAAACTTGTAATGGAAACGTTGCTTCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGa                                                                       <  1:1243004/150‑1 (MQ=255)
                                                                      cacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAg                                                                      <  1:646284/150‑1 (MQ=255)
                                                                             tCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGccgccg                                                               >  2:576566/1‑150 (MQ=255)
                                                                                 gCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAga                                                           >  2:785662/1‑150 (MQ=255)
                                                                                     tGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAgatgat                                                       <  1:1245082/150‑1 (MQ=255)
                                                                                     tGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAgatgat                                                       >  2:811569/1‑150 (MQ=255)
                                                                                      gCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATc                                                      <  2:1069598/150‑1 (MQ=255)
                                                                                          gggTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTa                                                  >  1:108658/1‑150 (MQ=255)
                                                                                            gTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACg                                                >  2:252847/1‑150 (MQ=255)
                                                                                              gAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGc                                              <  2:1133889/150‑1 (MQ=255)
                                                                                                aCTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGaa                                                                                              >  1:1126283/1‑100 (MQ=255)
                                                                                                aCTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGaa                                                                                              <  2:1126283/100‑1 (MQ=255)
                                                                                                 cTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAg                                           <  1:576566/150‑1 (MQ=255)
                                                                                                  tCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGCGGCCGCCGTAGATGATCGGTACGGCCAgg                                          >  1:564375/1‑150 (MQ=255)
                                                                                                      ttCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGt                                      >  2:349157/1‑150 (MQ=255)
                                                                                                          cTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTc                                     <  2:1054643/147‑1 (MQ=255)
                                                                                                          cTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTc                                     >  1:1054643/1‑147 (MQ=255)
                                                                                                           tCCAGGTTGCGCGACAGCTGCTTCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATc                                 >  1:45088/1‑150 (MQ=255)
                                                                                                           tCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAgatgat                                                       >  1:633482/1‑128 (MQ=255)
                                                                                                           tCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAgatgat                                                       <  2:633482/128‑1 (MQ=255)
                                                                                                                     gcgACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGt                       >  1:161210/1‑150 (MQ=255)
                                                                                                                           gctgctGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTAc                                                 <  2:110246/118‑1 (MQ=255)
                                                                                                                           gctgctGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTAc                                                 >  1:110246/1‑118 (MQ=255)
                                                                                                                           gctgctGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCa                 >  1:766682/1‑150 (MQ=255)
                                                                                                                                 gttcaTCGCCAATGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCttt           >  1:509314/4‑150 (MQ=255)
                                                                                                                                  ttcaTCGCCAATGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTGCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAgg                                          >  1:632730/3‑118 (MQ=255)
                                                                                                                                  ttcaTCGCCAATGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAgg                                          <  2:632730/116‑1 (MQ=255)
                                                                                                                                   acaTCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTcc         <  1:252847/150‑1 (MQ=255)
                                                                                                                                    caTCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCa        <  1:349157/150‑1 (MQ=255)
                                                                                                                                          cATTGGCGGTGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACa  >  1:836602/1‑150 (MQ=255)
                                                                                                                                          cATTGGCGATGAACTTGTCATGGAAGACTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACa  >  1:261884/1‑150 (MQ=255)
                                                                                                                                           aTTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGt                                      <  2:1036094/113‑1 (MQ=255)
                                                                                                                                           aTTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGt                                      >  1:1036094/1‑113 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACA  >  NC_002947/87353‑87640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑