Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,011,787 A→T 9.6% I66F (ATC→TTC)  prfC → peptide chain release factor 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,011,7870AT 9.6% 153.1 / 6.7 52I66F (ATC→TTC) prfCpeptide chain release factor 3
Reads supporting (aligned to +/- strand):  ref base A (19/28);  new base T (2/3);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

CCACCCCGACGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAA  >  NC_002947/1011645‑1011936
                                                                                                                                              |                                                                                                                                                     
ccACCCCGACGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCAt                                                                                                                                                <  1:190156/150‑1 (MQ=255)
 cACCCCGACGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGCCCATTGCCGCCGCCGGGACCGTGACGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGATGCCGCGCTGCTTCTCCAtt                                                                                                                                               <  1:678159/150‑2 (MQ=255)
    cccGACGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATcacc                                                                                                                                            >  1:1305935/1‑150 (MQ=255)
        aCGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCt                                                                                                                                        <  2:806583/150‑1 (MQ=255)
            cGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGt                                                                                                                                    <  1:102665/150‑1 (MQ=255)
              gTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGa                                                                                                                                  <  1:820250/150‑1 (MQ=255)
               tAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGAt                                                                                                                                 <  1:323243/150‑1 (MQ=255)
                aaGCCCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATg                                                                                                                                <  1:109382/150‑1 (MQ=255)
                        caTCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTccc                                                                                                                        >  1:335658/1‑150 (MQ=255)
                            aCCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTAc                                                                                                                    <  1:998531/150‑1 (MQ=255)
                             ccGGGAAGCTGCTGCTGATGGGCATGTACTTTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTAcc                                                                                                                   <  1:114/150‑1 (MQ=255)
                              cGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCAt                                                                                                                                                >  2:1335499/1‑120 (MQ=255)
                              cGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCAt                                                                                                                                                <  1:1335499/120‑1 (MQ=255)
                                      tgctgctgATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGcaca                                                                                                          >  1:1295085/1‑150 (MQ=255)
                                       gctgctgATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGATGCCGCGCTGCTTCTCCAtt                                                                                                                                               <  1:762494/112‑2 (MQ=255)
                                       gctgctgATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGATGCCGCGCTGCTTCTCCAtt                                                                                                                                               >  2:762494/1‑111 (MQ=255)
                                         tgctgATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGa                                                                                                       <  1:1234126/150‑1 (MQ=255)
                                         tgctgATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGa                                                                                                       <  2:171480/150‑1 (MQ=255)
                                         tgctcATCGGCAAGGCCATTGCCGTCGCCGGTTCCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGa                                                                                                       <  2:442256/150‑1 (MQ=255)
                                                 gcaacggCCA‑TGCCGTCGCCGATACCGGTAATTCGCGCAATTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGc                                                                                              <  2:306218/145‑1 (MQ=255)
                                                  gCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGc                                                                                              >  1:1149771/1‑150 (MQ=255)
                                                    aaGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTc                                                                                            >  2:220183/1‑150 (MQ=255)
                                                      ggCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTcc                                                                                                                         >  2:878850/1‑119 (MQ=255)
                                                      ggCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTcc                                                                                                                         <  1:878850/119‑1 (MQ=255)
                                                      ggCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACcgc                                                                                                                 >  1:1250778/1‑127 (MQ=255)
                                                      ggCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACcgc                                                                                                                 <  2:1250778/127‑1 (MQ=255)
                                                       gCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGac                                                                                         >  1:506985/1‑150 (MQ=255)
                                                            tGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACAcccc                                                                                    <  2:1295085/150‑1 (MQ=255)
                                                                     cGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGa                                                                           >  1:1160072/1‑150 (MQ=255)
                                                                      ggTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGaa                                                                          <  2:1160072/150‑1 (MQ=255)
                                                                      ggTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGaa                                                                          <  2:506985/150‑1 (MQ=255)
                                                                      ggTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGaa                                                                          <  2:1149771/150‑1 (MQ=255)
                                                                                  tCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGaa                                                              <  1:1146156/150‑1 (MQ=255)
                                                                                    gcgcAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGa                                                            <  1:650785/150‑1 (MQ=255)
                                                                                            ccGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTAcc                                                    <  1:243843/150‑1 (MQ=255)
                                                                                                   ccacgccacCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCa                                                 <  2:1177857/146‑1 (MQ=255)
                                                                                                   ccacgccacCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCa                                                 >  1:1177857/1‑146 (MQ=255)
                                                                                                    cacgccacCTCCGACTGGATGGAAATGAAGAAGCAGCGCGGCATCTCCATCACCACGTAGGTGATGCAGTTCGAGTAACGTAAAGTGGAATTGAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGACGCCACCTACCGCACCCTg                                            >  1:678928/1‑150 (MQ=255)
                                                                                                             tCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTg                                   <  2:1305935/150‑1 (MQ=255)
                                                                                                                     gatggaAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGa                                 >  2:1146369/1‑144 (MQ=255)
                                                                                                                     gatggaAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGa                                 <  1:1146369/144‑1 (MQ=255)
                                                                                                                      atggaAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGgcg                          >  1:64886/1‑150 (MQ=255)
                                                                                                                       tggaAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGgcgc                         >  1:752351/1‑150 (MQ=255)
                                                                                                                        ggaAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCt                        >  2:358183/1‑150 (MQ=255)
                                                                                                                            aTGGAGATGCCGCGCTGCTTCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATg                    >  2:823172/1‑150 (MQ=255)
                                                                                                                             tGGAGATGCCGCGCTGCTTCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATgg                   <  1:823172/150‑1 (MQ=255)
                                                                                                                               gagaAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTg                 >  2:466395/1‑150 (MQ=255)
                                                                                                                                agaAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCgg                            >  1:109700/1‑138 (MQ=255)
                                                                                                                                agaAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCgg                            <  2:109700/138‑1 (MQ=255)
                                                                                                                                agaAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGc                <  2:1353765/150‑1 (MQ=255)
                                                                                                                                       gcgcGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGCTCGAcgg         <  1:466395/150‑1 (MQ=255)
                                                                                                                                       gcgcGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGCTCGAcgg         >  1:171164/1‑150 (MQ=255)
                                                                                                                                              aTCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTaaa  <  2:752351/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CCACCCCGACGCCGGTAAGACCACCATCACCGAGAAGCTGCTGCTGATGGGCAAGGCCATTGCCGTCGCCGGTACCGTGAAGTCGCGCAAGTCCGACCGCCACGCCACCTCCGACTGGATGGAAATGGAGAAGCAGCGCGGCATCTCCATCACCACCTCGGTGATGCAGTTCCCGTACCGCGAGCACATGATCAACCTGCTCGACACCCCCGGCCACGAAGACTTCTCGGAAGACACCTACCGCACCCTGACCGCGGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAA  >  NC_002947/1011645‑1011936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑