| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,031,395 | G→C | 8.9% | S33T (AGC→ACC) | regA → | photosynthetic apparatus regulatory protein RegA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,031,395 | 0 | G | C | 8.9% | 166.1 / 7.3 | 56 | S33T (AGC→ACC) | regA | photosynthetic apparatus regulatory protein RegA |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base C (2/3); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
CACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACTGGACC > NC_002947/1031250‑1031544 | cACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGt < 1:1175484/150‑1 (MQ=255)cACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgg < 2:1005427/150‑2 (MQ=255) aCCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTc > 2:269066/1‑150 (MQ=255) aCTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCgt < 1:182438/150‑1 (MQ=255) cctcgcttGCCATAGGGCAGGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCgtg > 2:1339348/8‑150 (MQ=255) ctcgcTTGCCATAGGGCAGGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCgtgt > 1:823186/3‑150 (MQ=255) tcgcttGCCATAGGGCAGGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTGGCGGTTTCCGtgtg > 2:718507/6‑150 (MQ=255) cgcCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGa < 2:212077/150‑1 (MQ=255) cgcCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGa < 1:269066/150‑1 (MQ=255) ccTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgggt > 2:973718/1‑126 (MQ=255) ccTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgggt < 1:973718/129‑4 (MQ=255) agggCAGGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAg < 1:1339348/148‑1 (MQ=255) aaGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTg > 1:1131020/1‑150 (MQ=255) tgaatgaTGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGc < 1:1280030/150‑1 (MQ=255) tgaGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTc < 1:917752/150‑1 (MQ=255) gaGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCt > 2:328333/1‑150 (MQ=255) aGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTg < 2:1131020/150‑1 (MQ=255) aGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGACGAAGAAGGCTTGATTCTg < 1:1404711/150‑1 (MQ=255) gCGAAGAAAACCAGGTCGAAAGCGAATAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTgg < 2:293734/150‑1 (MQ=255) gaagaaAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTAATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGcc > 1:1153165/1‑150 (MQ=255) tCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGCGACGCCGCGGTTTCCGTTTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGc > 1:1032945/1‑150 (MQ=255) gAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTTCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCg < 2:823707/150‑1 (MQ=255) gAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCg < 2:823186/150‑1 (MQ=255) gAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTAc > 1:151770/1‑150 (MQ=255) gagCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGcc > 2:689119/1‑150 (MQ=255) ccGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTg < 1:328333/150‑1 (MQ=255) tgctgGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTgg < 2:7648/108‑1 (MQ=255) tgctgGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTgg > 1:7648/1‑108 (MQ=255) tggtggATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTg > 1:40459/1‑104 (MQ=255) tggtggATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTg < 2:40459/104‑1 (MQ=255) aTGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat < 1:896573/146‑1 (MQ=255) aTGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat > 2:896573/1‑146 (MQ=255) tGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat > 1:709169/1‑145 (MQ=255) tGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat < 2:709169/145‑1 (MQ=255) gATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTcc > 2:842515/1‑150 (MQ=255) ccACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGcc > 2:1287330/1‑150 (MQ=255) aCCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTg < 2:151770/150‑1 (MQ=255) ggcccgggccatgacccgggccatgaGCCGTCGCGGATTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGtgct > 2:534106/3‑150 (MQ=255) cACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGtgct < 2:1234188/150‑1 (MQ=255) cccGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCGACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGtgctgc < 2:1032945/150‑1 (MQ=255) ggctCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTg > 1:105183/4‑140 (MQ=255) ggctCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTg < 2:105183/137‑1 (MQ=255) ggctCATGGCCCGGGCCATGACCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCca > 2:1167255/4‑82 (MQ=255) ggctCATGGCCCGGGCCATGACCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCca < 1:1167255/79‑1 (MQ=255) gTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGccc > 1:640628/1‑150 (MQ=255) aTGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAg > 2:735668/1‑150 (MQ=255) cccGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAgctgc > 2:995192/1‑150 (MQ=255) ccGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAgctgct > 2:904591/1‑150 (MQ=255) cGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat > 2:1271761/1‑114 (MQ=255) cGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat > 2:1271632/1‑114 (MQ=255) cGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat < 1:1271761/114‑1 (MQ=255) cGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat < 1:1271632/114‑1 (MQ=255) gCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGaa < 1:1287330/150‑1 (MQ=255) ccATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAAc < 2:1153165/150‑1 (MQ=255) cATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACt > 1:1251904/1‑150 (MQ=255) aTGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACTg > 1:1354205/1‑150 (MQ=255) gCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACTGGAcc < 1:995192/150‑1 (MQ=255) | CACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACTGGACC > NC_002947/1031250‑1031544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |