Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,076,841 G→C 7.2% R72P (CGC→CCC)  mreC → cell shape‑determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,076,8410GC 7.2% 173.1 / 3.1 56R72P (CGC→CCC) mreCcell shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base C (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCACCGCATTCTGATCAACAA  >  NC_002947/1076698‑1076988
                                                                                                                                               |                                                                                                                                                   
gggCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCa                                                                                                                                               <  2:35564/150‑1 (MQ=255)
  gCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTTCTGCAGGCGCCCCTGCAgc                                                                                                                                             >  1:458014/1‑149 (MQ=255)
       gTGCTGATGGAGTCGTACTGGATCATCCGCTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTTCTGCAGGCGCCCCTGCagcagcag                                                                                                                                        <  1:1071579/150‑3 (MQ=255)
           tGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggcgg                                                                                                                                    >  2:941590/1‑150 (MQ=255)
             aTGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGcc                                                                                                                                  >  1:1060206/1‑150 (MQ=255)
             aTGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGcc                                                                                                                                  >  2:465924/1‑150 (MQ=255)
              tGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGccc                                                                                                                                 <  2:48393/150‑1 (MQ=255)
               ggAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGACGCTAAAGACCGAAGCCCTGCTGCTGCAGGGGCTCCTGCAGAAGCTGGCGGCCCt                                                                                                                                <  2:1231606/150‑1 (MQ=255)
                           gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGaa                                                                                                                    >  1:1288848/1‑150 (MQ=255)
                            aTCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAGACGATCAGCACAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAAc                                                                                                                   >  1:798845/1‑150 (MQ=255)
                               aCCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCAGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                                                                                                                >  1:104294/1‑150 (MQ=255)
                                 cGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCg                                                                                                              >  2:1126262/1‑150 (MQ=255)
                                        ccACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgcgc                                                                                                       >  1:1399019/1‑150 (MQ=255)
                                          acaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGa                                                                                                     >  1:425406/1‑150 (MQ=255)
                                             gCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGtt                                                                                                  <  1:289670/150‑1 (MQ=255)
                                                         aGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTc                                                                                      <  2:1399019/150‑1 (MQ=255)
                                                            tgtgGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGGAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGgc                                                                                   >  1:329016/1‑150 (MQ=255)
                                                            tgtgGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGgc                                                                                   >  1:1005692/1‑150 (MQ=255)
                                                            tgtgGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCCAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGgc                                                                                   >  1:889864/1‑150 (MQ=255)
                                                                gccggccAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTg                                                                               <  1:485204/150‑1 (MQ=255)
                                                                  cggccAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGTGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGt                                                                             >  2:664259/1‑150 (MQ=255)
                                                                     ccAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGa                                                                                                                          <  1:411847/102‑1 (MQ=255)
                                                                     ccAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGa                                                                                                                          >  2:411847/1‑102 (MQ=255)
                                                                      cAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                                                                                                                                      >  1:1225479/1‑89 (MQ=255)
                                                                      cAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                                                                                                                                      <  2:1225479/89‑1 (MQ=255)
                                                                      cAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAAc                                                                         <  2:1288848/150‑1 (MQ=255)
                                                                               aGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGtt                                                                <  1:1126262/150‑1 (MQ=255)
                                                                                  cGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAg                                                                                                    >  1:781761/1‑111 (MQ=255)
                                                                                  cGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAg                                                                                                    <  2:781761/111‑1 (MQ=255)
                                                                                    cACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGt                                                           >  1:207812/1‑150 (MQ=255)
                                                                                     aCCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTg                                                          >  2:1131024/1‑150 (MQ=255)
                                                                                      ccGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTgg                                                         >  2:322927/1‑150 (MQ=255)
                                                                                       cGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGTGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGc                                                        <  1:664259/150‑1 (MQ=255)
                                                                                         aGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCg                                                      >  2:1253833/1‑150 (MQ=255)
                                                                                          gCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGa                                                     <  1:1326583/150‑1 (MQ=255)
                                                                                            tGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGc                                                   <  1:941590/150‑1 (MQ=255)
                                                                                            tGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCACAACGTGCGGCTGCGCGAGTTGCTCACCTCTTCGGCGCTGGTCAACGACAAGGTTGAAGTGGCCCAGc                                                   <  2:433777/150‑1 (MQ=255)
                                                                                              aTTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAg                                                            >  1:451006/1‑139 (MQ=255)
                                                                                              aTTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAg                                                            <  2:451006/139‑1 (MQ=255)
                                                                                               ttGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGTGCAGAACGTGCGGCTGCGCTAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGa                                                <  1:465924/150‑1 (MQ=255)
                                                                                                 gCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATc                                              <  2:207812/150‑1 (MQ=255)
                                                                                                    gAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGgt                                           <  1:1253833/150‑1 (MQ=255)
                                                                                                     aaaaCGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGt                                                                                                   >  2:1292279/1‑93 (MQ=255)
                                                                                                     aaaaCGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGt                                                                                                   <  1:1292279/93‑1 (MQ=255)
                                                                                                      aaaCGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGgtgt                                         >  1:1030743/1‑150 (MQ=255)
                                                                                                          gagaAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgcgc                                                                                                       <  1:801192/84‑1 (MQ=255)
                                                                                                          gagaAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgcgc                                                                                                       >  2:801192/1‑84 (MQ=255)
                                                                                                          gagaAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCg                                             >  2:449331/1‑142 (MQ=255)
                                                                                                          gagaAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCg                                             <  1:449331/142‑1 (MQ=255)
                                                                                                                cTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTTTCGACCCCTAc                               <  2:798845/150‑1 (MQ=255)
                                                                                                                       ccGAAGCCCTGCTGCCGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTa                        <  1:161653/150‑1 (MQ=255)
                                                                                                                           gccccTGCAGCAGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCa                    >  2:84927/3‑150 (MQ=255)
                                                                                                                            gCCCTGCTGCTGCAGGGGCCCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCAc                   >  1:1294479/1‑150 (MQ=255)
                                                                                                                                   tgctgcAGGGGCGCCTGCAGACCCTGACGGCCGTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCACCGCATTc            <  2:889864/150‑1 (MQ=255)
                                                                                                                                       gcAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCACCGCATTCTGAt        <  2:329016/150‑1 (MQ=255)
                                                                                                                                        cAGGCGCCCCTGCAGCAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAg                                                    <  1:630777/105‑1 (MQ=255)
                                                                                                                                             gcgcCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCACCGCATTCTGATcaacaa  <  1:1131024/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCGGCGCTGGTCAACGAAAAGGTTGAAGTGGCCGAGCTGATCGGTGTCGACCCCAACCCGTTTACCCACCGCATTCTGATCAACAA  >  NC_002947/1076698‑1076988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑