Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,110,375 A→T 7.4% R132R (CGA→CGT) ‡ PP_0970 → cyanate MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,110,3750AT 7.4% 166.0 / 4.7 54R132R (CGA→CGT) ‡PP_0970cyanate MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base T (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

ATGCTTCGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATACACCATGGCGTTAT  >  NC_002947/1110227‑1110523
                                                                                                                                                    |                                                                                                                                                    
aTGCTTCGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGaa                                                                                                                                                     <  1:187831/150‑1 (MQ=255)
 tGCTTCGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGCGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAg                                                                                                                                                    <  1:758141‑M2/150‑2 (MQ=255)
    ttCGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGGGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACATGGCCTCGTTGTACGAAgtac                                                                                                                                                 <  2:732262‑M2/150‑5 (MQ=255)
      cGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCt                                                                                                                                               <  2:744473/150‑1 (MQ=255)
        cAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCttt                                                                                                                                             <  1:1224697/150‑1 (MQ=255)
          ggctggctTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTgg                                                                                                                                           <  1:1398092/150‑1 (MQ=255)
           gctggctTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTggg                                                                                                                                          <  1:95430/150‑1 (MQ=255)
                 ttGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATc                                                                                                                                    >  2:1213584/1‑150 (MQ=255)
                   gCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCgg                                                                                                                                  <  2:171979/150‑1 (MQ=255)
                            cTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATTGGGGTGTTCCt                                                                                                                         >  1:229508/1‑150 (MQ=255)
                              tGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCg                                                                                                                       <  2:951498/150‑1 (MQ=255)
                                 cGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGCGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCa                                                                                                                    <  2:758141/150‑1 (MQ=255)
                                   gTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGc                                                                                                                  <  1:1213584/150‑1 (MQ=255)
                                      ctctGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTg                                                                                                               >  1:352997/1‑150 (MQ=255)
                                            cTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATTGCg                                                                                                         >  1:50995/1‑150 (MQ=255)
                                                     tttGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGc                                                                                                >  1:41431/1‑150 (MQ=255)
                                                        gCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATc                                                                                             <  1:212358/150‑1 (MQ=255)
                                                          tCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCgg                                                                                           >  1:1196159/1‑150 (MQ=255)
                                                          tCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCgg                                                                                           >  1:368810/1‑150 (MQ=255)
                                                            ccTTGGCGCCGGTGCTGGCGCGGCGCTTTGTCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCa                                                                                         >  1:832895/1‑150 (MQ=255)
                                                            ccTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCttt                                                                                                                                             <  1:469570/98‑1 (MQ=255)
                                                            ccTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCttt                                                                                                                                             >  2:469570/1‑98 (MQ=255)
                                                                       gTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTa                                                                              >  1:47704/1‑150 (MQ=255)
                                                                         gCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTAtt                                                                            <  1:663177/150‑1 (MQ=255)
                                                                                cggcgcTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCTCTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTAccc                                                                     <  2:475407/150‑1 (MQ=255)
                                                                                     cTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTAt                                                                <  2:284644/150‑1 (MQ=255)
                                                                                           caacgagcgGGTGATTCTTGGCATCCTCCTCACCCTCGCATTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCaa                                                          <  1:653917/147‑1 (MQ=255)
                                                                                               gagcgGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAgcgc                                                      <  2:50995/150‑1 (MQ=255)
                                                                                                  cgGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGAc                                                   <  2:368810/150‑1 (MQ=255)
                                                                                                  cgGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGACCGGCATCGGGATTAGCGGCGTATTGTTACCCGGTATCGTCAAGCGCGAc                                                   <  1:1316412/150‑1 (MQ=255)
                                                                                                    ggTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCggg                                                                                                                                 >  2:1070060/1‑70 (MQ=255)
                                                                                                    ggTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCggg                                                                                                                                 <  1:1070060/70‑1 (MQ=255)
                                                                                                     gTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCaa                                                          <  2:1177714/140‑1 (MQ=255)
                                                                                                     gTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCaa                                                          >  1:1177714/1‑140 (MQ=255)
                                                                                                      tGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCACTAGGGGTGTTCATCGGCAGACTGATGGCGGGCGCAAGCCTCGGCACTATCGGAGTATTGTTAGCCGGTACCGTCAAGcgcggcatgc                                               >  2:593360/1‑144 (MQ=255)
                                                                                                      tGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTc                                               <  2:832895/150‑1 (MQ=255)
                                                                                                       gATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCTACTTTcc                                              >  1:794718/1‑150 (MQ=255)
                                                                                                          tCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACTTCgtaccacgag                                                                                                                                           >  1:468843‑M2/1‑44 (MQ=255)
                                                                                                          tCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACTTCgtaccacgag                                                                                                                                           <  2:468843‑M2/54‑11 (MQ=255)
                                                                                                            ttGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACTTCgtaccacgag                                                                                                                                           <  2:1208812‑M2/52‑11 (MQ=255)
                                                                                                            ttGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACTTCgtaccacgag                                                                                                                                           >  1:1208812‑M2/1‑42 (MQ=255)
                                                                                                              ggCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATc                                                                                                                                    <  1:1067449/57‑1 (MQ=255)
                                                                                                              ggCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATc                                                                                                                                    >  2:1067449/1‑57 (MQ=255)
                                                                                                                  tcctccTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAgcacgctg                                   <  2:41431/150‑1 (MQ=255)
                                                                                                                  tcctccTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAgcacgctg                                   >  2:257948/1‑150 (MQ=255)
                                                                                                                     tcctcACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCg                                                                    <  1:1288650/114‑1 (MQ=255)
                                                                                                                     tcctcACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCg                                                                    >  2:1288650/1‑114 (MQ=255)
                                                                                                                        tcACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATAATCGTCTTATTGTTAAACGGTATCGTCAAGCGCGACTTTCCACAgcaagctggcacgc                             >  2:690833/1‑150 (MQ=255)
                                                                                                                                      ggCCTTGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATac               >  1:790195/1‑150 (MQ=255)
                                                                                                                                        ccTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATacac             >  1:137355/1‑150 (MQ=255)
                                                                                                                                         cTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATACAcc            >  2:1227627/1‑150 (MQ=255)
                                                                                                                                         cTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGGATCGTCAAGCGCGACTTTCCACAGCACGCTGGCCCGCTGACCGGTGTATACAcc            >  2:515756/1‑150 (MQ=255)
                                                                                                                                          tCGTGGTACGAAgtaccacgaggccaagtgccagggtgaggaggatgccaagaatcacccgctcgctgccaaagcgccgcgccagcaccggcgccaagggagcaaac                                                      >  1:193151‑M2/1‑12 (MQ=255)
                                                                                                                                          tCGTGGTACGAAgtaccacgaggccaagtgccagggtgaggaggatgccaagaatcacccgctcgctgccaaagcgccgcgccagcaccggcgccaagggagcaaac                                                      <  2:193151‑M2/107‑96 (MQ=255)
                                                                                                                                                   gAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATACACCATGGCGTTAt  <  2:137355/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGCTTCGCAGGCTGGCTTGCTGACCACCTTGCCGGTGCTCTGCCTGGGCCTGTTTGCTCCCTTGGCGCCGGTGCTGGCGCGGCGCTTTGGCAGCGAGCGGGTGATTCTTGGCATCCTCCTCACCCTGGCACTTGGCCTCGTGGTACGAAGTACCTTTGGGGCAATCGGGGTGTTCCTCGGCAGCCTGATGGCGGGGGCCAGCATCGGCATTATCGGCGTATTGTTACCCGGTATCGTCAAGCGCGACTTTCCACAGCACGCTGGCACGCTGACCGGTGTATACACCATGGCGTTAT  >  NC_002947/1110227‑1110523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑