Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,450 A→G 9.3% N296D (AAC→GAC)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4500AG 9.3% 117.2 / 3.0 43N296D (AAC→GAC) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base A (20/19);  new base G (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

GAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTG  >  NC_002947/1121303‑1121599
                                                                                                                                                    |                                                                                                                                                     
gAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAAc                                                                                                                                                     <  2:633749/150‑1 (MQ=255)
                 cgAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGATCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCg                                                                                                                                       >  1:1273370/1‑147 (MQ=255)
                 cgAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGATCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCg                                                                                                                                       <  2:1273370/147‑1 (MQ=255)
                    aGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCt                                                                                                                                 <  1:62958/150‑1 (MQ=255)
                         ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                            >  1:551201/1‑150 (MQ=255)
                         ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                            >  1:506162/1‑150 (MQ=255)
                                cTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCAGGCCAGGGCCGCTTCCTCAAGCTGCTGcc                                                                                                                     >  1:1278/1‑150 (MQ=255)
                                        tGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                             >  1:515472/1‑150 (MQ=255)
                                          aTCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttctt                                                                                                           >  2:1100697/1‑150 (MQ=255)
                                                       gCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                              >  2:107799/1‑150 (MQ=255)
                                                        ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTa                                                                                             >  1:1285679/1‑150 (MQ=255)
                                                          ttctcgAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGATGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCTCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCCATTCTTCTGTACATGCACTAcc                                                                                           >  2:286142/6‑150 (MQ=255)
                                                                  cgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCaa                                                                                                                              >  1:557973/1‑107 (MQ=255)
                                                                  cgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCaa                                                                                                                              <  2:557973/107‑1 (MQ=255)
                                                                    cgcCGAGCTGAAATGGTGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCCACCGCACCGAG‑GGCTGGCATGGGGCAACCCGCGCCAACGCCGCTCCCTCAAGATGCTGCCTGTAATTCTTCTGTACATGGCCTACCTGACAATGCt                                                                                 <  1:678812/150‑1 (MQ=255)
                                                                        gAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGAtt                                                                             <  1:489277/150‑1 (MQ=255)
                                                                         aGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCACCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                               <  1:809868/147‑1 (MQ=255)
                                                                         aGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                               >  2:809868/1‑147 (MQ=255)
                                                                            tGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCg                                                                         >  2:551481/1‑150 (MQ=255)
                                                                                   gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                        <  1:266094/96‑1 (MQ=255)
                                                                                   gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                        >  2:266094/1‑96 (MQ=255)
                                                                                    cGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcggc                                                                 <  2:551201/150‑1 (MQ=255)
                                                                                       ttGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGcc                                                              <  2:593106/150‑1 (MQ=255)
                                                                                               gCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGaa                                                      <  1:286142/150‑1 (MQ=255)
                                                                                                ccGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAg                                                     <  2:405417/150‑1 (MQ=255)
                                                                                                           gtTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                              <  1:341577/98‑1 (MQ=255)
                                                                                                           gtTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                              >  2:341577/1‑98 (MQ=255)
                                                                                                             tcgtcgTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                                        <  1:551481/150‑1 (MQ=255)
                                                                                                              cgtcgtCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                               >  1:1244784/1‑110 (MQ=255)
                                                                                                              cgtcgtCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                               <  2:1244784/110‑1 (MQ=255)
                                                                                                                  gtcACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGcc                                   >  2:1314461/1‑150 (MQ=255)
                                                                                                                    cACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCt                                 <  2:1325612/150‑1 (MQ=255)
                                                                                                                       ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                               >  2:138536/1‑85 (MQ=255)
                                                                                                                       ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                               <  1:138536/85‑1 (MQ=255)
                                                                                                                       ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTggg                              >  2:158744/1‑150 (MQ=255)
                                                                                                                           ctgG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtg                          >  2:414277/1‑150 (MQ=255)
                                                                                                                            tgG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtgt                         <  1:107799/150‑1 (MQ=255)
                                                                                                                                      cGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTcc                >  1:980444/1‑150 (MQ=255)
                                                                                                                                        ctggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAAt                                                                                    <  2:754861/76‑1 (MQ=37)
                                                                                                                                        ctggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                       <  2:495352/137‑1 (MQ=255)
                                                                                                                                        cTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAAt                                                                                    >  1:754861/1‑80 (MQ=38)
                                                                                                                                        cTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                       >  1:495352/1‑141 (MQ=255)
                                                                                                                                                 gTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTc     <  1:414277/150‑1 (MQ=255)
                                                                                                                                                    aaCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCctg  <  1:1314461/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTG  >  NC_002947/1121303‑1121599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑