Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,458 C→A 9.1% R298R (CGC→CGA)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4580CA 9.1% 124.6 / 3.3 44R298R (CGC→CGA) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base A (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

CGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTGCTGATCGG  >  NC_002947/1121320‑1121607
                                                                                                                                           |                                                                                                                                                     
cgAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGATCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCg                                                                                                                                               >  1:1273370/1‑147 (MQ=255)
cgAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGATCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCg                                                                                                                                               <  2:1273370/147‑1 (MQ=255)
   aGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCt                                                                                                                                         <  1:62958/150‑1 (MQ=255)
        ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                                    >  1:551201/1‑150 (MQ=255)
        ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                                    >  1:506162/1‑150 (MQ=255)
               cTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCAGGCCAGGGCCGCTTCCTCAAGCTGCTGcc                                                                                                                             >  1:1278/1‑150 (MQ=255)
                       tGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                                     >  1:515472/1‑150 (MQ=255)
                         aTCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttctt                                                                                                                   >  2:1100697/1‑150 (MQ=255)
                                      gCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                                      >  2:107799/1‑150 (MQ=255)
                                       ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTa                                                                                                     >  1:1285679/1‑150 (MQ=255)
                                         ttctcgAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGATGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCTCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCCATTCTTCTGTACATGCACTAcc                                                                                                   >  2:286142/6‑150 (MQ=255)
                                                 cgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCaa                                                                                                                                      >  1:557973/1‑107 (MQ=255)
                                                 cgcgcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCaa                                                                                                                                      <  2:557973/107‑1 (MQ=255)
                                                   cgcCGAGCTGAAATGGTGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCCACCGCACCGAG‑GGCTGGCATGGGGCAACCCGCGCCAACGCCGCTCCCTCAAGATGCTGCCTGTAATTCTTCTGTACATGGCCTACCTGACAATGCt                                                                                         <  1:678812/150‑1 (MQ=255)
                                                       gAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGAtt                                                                                     <  1:489277/150‑1 (MQ=255)
                                                        aGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCACCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                                       <  1:809868/147‑1 (MQ=255)
                                                        aGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                                       >  2:809868/1‑147 (MQ=255)
                                                           tGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCg                                                                                 >  2:551481/1‑150 (MQ=255)
                                                                  gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                                >  2:266094/1‑96 (MQ=255)
                                                                  gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                                <  1:266094/96‑1 (MQ=255)
                                                                   cGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcggc                                                                         <  2:551201/150‑1 (MQ=255)
                                                                      ttGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGcc                                                                      <  2:593106/150‑1 (MQ=255)
                                                                              gCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGaa                                                              <  1:286142/150‑1 (MQ=255)
                                                                               ccGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAg                                                             <  2:405417/150‑1 (MQ=255)
                                                                                          gtTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                                      >  2:341577/1‑98 (MQ=255)
                                                                                          gtTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                                      <  1:341577/98‑1 (MQ=255)
                                                                                            tcgtcgTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                                                <  1:551481/150‑1 (MQ=255)
                                                                                             cgtcgtCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                                       <  2:1244784/110‑1 (MQ=255)
                                                                                             cgtcgtCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGa                                                                                       >  1:1244784/1‑110 (MQ=255)
                                                                                                 gtcACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGcc                                           >  2:1314461/1‑150 (MQ=255)
                                                                                                   cACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCt                                         <  2:1325612/150‑1 (MQ=255)
                                                                                                      ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                                       <  1:138536/85‑1 (MQ=255)
                                                                                                      ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                                       >  2:138536/1‑85 (MQ=255)
                                                                                                      ccTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTggg                                      >  2:158744/1‑150 (MQ=255)
                                                                                                          ctgG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtg                                  >  2:414277/1‑150 (MQ=255)
                                                                                                           tgG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtgt                                 <  1:107799/150‑1 (MQ=255)
                                                                                                                     cGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTcc                        >  1:980444/1‑150 (MQ=255)
                                                                                                                       ctggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAAt                                                                                            <  2:754861/76‑1 (MQ=37)
                                                                                                                       ctggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                               <  2:495352/137‑1 (MQ=255)
                                                                                                                       cTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAAt                                                                                            >  1:754861/1‑80 (MQ=38)
                                                                                                                       cTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                               >  1:495352/1‑141 (MQ=255)
                                                                                                                                gTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTc             <  1:414277/150‑1 (MQ=255)
                                                                                                                                   aaCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCctg          <  1:1314461/150‑1 (MQ=255)
                                                                                                                                      ccGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGAATGTGGTGGGTCCACGGCCTGTTCctgctg       >  1:714393/1‑150 (MQ=255)
                                                                                                                                           ccAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTGCTGATctg  >  1:243332/1‑148 (MQ=255)
                                                                                                                                           ccAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTGCTGATCgg  >  2:1322689/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                     
CGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGG‑CGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCTGCTGATCGG  >  NC_002947/1121320‑1121607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑