| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 124,515 | T→A | 8.8% | N40I (AAT→ATT) | znuC ← | zinc ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 124,515 | 0 | T | A | 8.8% | 164.7 / 7.5 | 57 | N40I (AAT→ATT) | znuC | zinc ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base A (3/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01 | |||||||||||
GCGCAGCACCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCC > NC_002947/124367‑124662 | gcgcAGCACCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCAtt < 1:279414/150‑1 (MQ=255) cagcaCCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTggg < 1:1068571/150‑1 (MQ=255) cagcaCCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTggg < 2:1226316/150‑1 (MQ=255) cagcaCCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCAATggg > 1:1286320/1‑150 (MQ=255) gcaCCGACAGCGGCAGTGTGGCATCAAATTTAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGc < 1:1072631/150‑1 (MQ=255) ccGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCaa < 1:868673/150‑1 (MQ=255) ccGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCAATGGGGcagg < 2:1286320/150‑4 (MQ=255) cGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGATCGTCTTGCCTGCCCCCTTGGGGCCaaa > 2:300899/1‑149 (MQ=255) gCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGa < 1:107634/150‑1 (MQ=255) cAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGAc < 2:569258/150‑1 (MQ=255) aaGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGAc < 1:734597/149‑1 (MQ=255) gTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATc < 2:744712/150‑1 (MQ=255) cATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGc > 1:144881/1‑150 (MQ=255) tCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCt > 1:989379/1‑150 (MQ=255) tCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTGGCCGCGATGTGGCTTGAGCAGGGCAAGTACGGCGCGCACCAGGGTCGTCTTGCCCGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCt < 2:1400104/150‑1 (MQ=255) tGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGcg > 2:249778/1‑150 (MQ=255) gAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATc > 2:391250/1‑125 (MQ=255) gAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATc < 1:391250/125‑1 (MQ=255) ttctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCt > 1:500621/1‑133 (MQ=255) ttctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCt < 2:500621/133‑1 (MQ=255) ttctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGcgaccgac > 2:704749/1‑150 (MQ=255) ttctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCAATGGGGcag > 2:191090/1‑115 (MQ=255) ttctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCAATGGGGcag < 1:191090/117‑3 (MQ=255) ctGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAg < 1:267863/150‑1 (MQ=255) gCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTggg < 2:1129299/109‑1 (MQ=255) gCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTggg > 1:1129299/1‑109 (MQ=255) ccAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGc < 2:696233/100‑1 (MQ=255) ccAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGc > 1:696233/1‑100 (MQ=255) gcAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTg < 1:238321/89‑1 (MQ=255) gcAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTg > 2:238321/1‑89 (MQ=255) gcgcCATACCTTGCCGCGATGTGGCTTGAGCAGGCCCAGTTCGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCaaa < 1:75092/150‑1 (MQ=255) cgcCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCTCCAAAg < 2:144881/150‑1 (MQ=255) cgcCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAg < 1:611475/150‑1 (MQ=255) ttGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGt > 2:863619/1‑118 (MQ=255) ttGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGt < 1:863619/118‑1 (MQ=255) ttGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCg > 2:807434/1‑150 (MQ=255) cgcgATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACAAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCt > 1:817893/1‑150 (MQ=255) aTGTTGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCCACCGACATGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATc < 2:26663/150‑1 (MQ=255) gCTTGAGCAGGCCAAGTACGGCGCGCACAAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGc < 2:817893/150‑1 (MQ=255) cAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAg > 1:580690/1‑150 (MQ=255) aGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCCTGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAgg < 2:1261533/150‑1 (MQ=255) gCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGc > 1:870064/1‑150 (MQ=255) gcgcgcACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCa < 2:941535/136‑1 (MQ=255) gcgcgcACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCa > 1:941535/1‑136 (MQ=255) aGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTcc < 2:461729/150‑1 (MQ=255) gcgtcTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCa < 1:300899/149‑1 (MQ=255) tcTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGtt > 1:924039/1‑150 (MQ=255) tcTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATAAGGGCGTCGCTCATGCCGCGCTCCGGCAGtt > 1:1159840/1‑150 (MQ=255) cTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAggg < 1:1364791/122‑1 (MQ=255) cTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAggg > 2:1364791/1‑122 (MQ=255) ttGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCa < 2:333596/109‑1 (MQ=255) ttGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCa > 1:333596/1‑109 (MQ=255) ttGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGAACCGTCCGAACACGGAAGGTGACGCCGACCTGATCCAGGCGAACCAGGCCGAACACCATGCCGCCCTCCGGCAGTGAc > 1:1022514/1‑150 (MQ=255) ttggccCCAATGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGc > 2:278845/4‑150 (MQ=255) ctgccCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGca > 2:418758/1‑150 (MQ=255) ccccATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAg > 2:617718/1‑150 (MQ=255) cccATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAgg > 2:30274/1‑150 (MQ=255) cATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGcc > 2:546534/1‑150 (MQ=255) | GCGCAGCACCGACAGCGGCAGCGTGGCATCAACCTGAATCTTCTGCGGCATGTAGCCAATGCGCAGCTTGGGCTTGCGCCATACCTTGCCGCGATGTGGCTTGAGCAGGCCAAGTACGGCGCGCACCAGGGTCGTCTTGCCTGCCCCATTGGGGCCAATCAGGGTGACGATCTGGCCTGGGGCGACCGACAGGTCGATGCTGTCGAGCACCGCCTCGCCGCCAAAGGTGACGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCC > NC_002947/124367‑124662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |