| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,289,857 | A→T | 9.3% | N375I (AAT→ATT) | PP_1126 → | hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,289,857 | 0 | A | T | 9.3% | 124.4 / 4.1 | 43 | N375I (AAT→ATT) | PP_1126 | hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (23/16); new base T (2/2); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGC > NC_002947/1289708‑1290005 | gCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCaa < 2:800243/150‑1 (MQ=255) ccGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGcc > 2:331013/1‑150 (MQ=255) tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtcg > 2:676823/1‑150 (MQ=255) tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc > 2:656983/1‑150 (MQ=255) gCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAg > 1:768380/1‑150 (MQ=255) tGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAgg < 1:217645/150‑1 (MQ=255) ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc > 1:153108/1‑123 (MQ=255) ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc < 2:153108/123‑1 (MQ=255) cggcggGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTAGGAGATTCTCAATTTCAAGCAGGGGCGCTGGtt < 1:1107037/150‑1 (MQ=255) aCCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAc > 1:1011539/1‑150 (MQ=255) gcTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAcg > 2:379800/2‑150 (MQ=255) cGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCaa < 1:646086/150‑1 (MQ=255) gCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAc < 1:128840/150‑1 (MQ=255) gCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAc < 1:379800/150‑1 (MQ=255) ggCGCGCAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGc > 1:1135940/1‑150 (MQ=255) cgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAACCTCGGGTGCGGGTTCGACTTCTACGCCGCCAACCTGCAGCg > 2:473655/1‑150 (MQ=255) gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGt > 1:498775/1‑150 (MQ=255) gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc < 1:826046/89‑1 (MQ=255) gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtc > 2:826046/1‑89 (MQ=255) aGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc < 1:331013/150‑1 (MQ=255) gCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg > 1:361855/1‑91 (MQ=255) gCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg < 2:361855/91‑1 (MQ=255) gTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATgg > 2:547478/1‑150 (MQ=255) ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGTGAAAGCGACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc < 2:866844/150‑1 (MQ=255) ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATTGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCCACCTGCAGCGTACCTATGGCAAACCCTGc < 1:495469/150‑1 (MQ=255) cacGCTGAGGGCGATGCACTGGGCGCTGTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCgg > 2:313342/3‑150 (MQ=255) gCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGccc < 1:676823/150‑1 (MQ=255) gCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTgg > 2:1067601/1‑150 (MQ=255) cGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGa < 1:586318/150‑1 (MQ=255) cGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGa < 1:656983/150‑1 (MQ=255) aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca < 1:1067601/150‑1 (MQ=255) cTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGTCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc > 1:853138/1‑113 (MQ=255) cTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc < 2:853138/113‑1 (MQ=255) cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc > 2:1386693/1‑150 (MQ=255) gCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATgg > 2:819354/1‑113 (MQ=255) gCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATgg < 1:819354/113‑1 (MQ=255) gCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgcgc > 2:1054102/1‑150 (MQ=255) caacaaCGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGt > 2:933572/1‑150 (MQ=255) caacaaCGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGt > 1:1010931/1‑150 (MQ=255) aacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTg > 1:786673/1‑150 (MQ=255) cgacgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGa > 2:608935/1‑150 (MQ=255) gacgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGAt > 1:122490/1‑150 (MQ=255) gtGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCg < 2:122490/149‑1 (MQ=255) cAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCg > 2:1334412/1‑150 (MQ=255) aaTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc < 2:1180302/116‑1 (MQ=255) aaTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc > 1:1180302/1‑116 (MQ=255) aaTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGGCCGc > 1:948893/1‑150 (MQ=255) aaTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTCGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGc > 2:843977/1‑150 (MQ=255) | GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGC > NC_002947/1289708‑1290005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |