Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,308,833 T→A 9.5% L116Q (CTG→CAG)  PP_1143 → 3‑hydroxyisobutyrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,308,8330TA 9.5% 222.8 / 12.6 74L116Q (CTG→CAG) PP_11433‑hydroxyisobutyrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (32/35);  new base A (4/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

TGTGCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAGCGCGTGACGC  >  NC_002947/1308686‑1308979
                                                                                                                                                   |                                                                                                                                                  
tgtgCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgt                                                                                                                                                  >  1:233944/1‑150 (MQ=255)
tgtgCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgt                                                                                                                                                  >  2:968424/1‑150 (MQ=255)
tgtgCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgt                                                                                                                                                  >  1:942893/1‑150 (MQ=255)
tgtgCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCAGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgt                                                                                                                                                  >  1:331652/1‑150 (MQ=255)
    ccTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCgg                                                                                                                                              >  2:391238/1‑150 (MQ=255)
     cTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGc                                                                                                                                             >  1:220263/1‑150 (MQ=255)
      tGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCa                                                                                                                                            >  2:1170973/1‑150 (MQ=255)
       ggCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCAt                                                                                                                                           >  2:1246469/1‑150 (MQ=255)
                ggcggTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCctggct                                                                                                                                  <  1:1219909/150‑1 (MQ=255)
                ggcggTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCctggct                                                                                                                                  <  1:659717/150‑1 (MQ=255)
                   ggtggtGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGa                                                                                                                               <  2:580718/150‑1 (MQ=255)
                   ggtggtGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCACCGGCCAGTTGCTGAGCGATTTCTCCAGCCTGGAACCCACCGCCACCCGGGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGa                                                                                                                               <  1:452211/150‑1 (MQ=255)
                            tGAAGTGGTGTTCGACGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCGGTGGCTGGTCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCggg                                                                                                                      >  2:1123054/1‑149 (MQ=255)
                             gAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTa                                                                                                                     >  1:1051358/1‑150 (MQ=255)
                              aaGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTAt                                                                                                                    <  1:149068/150‑1 (MQ=255)
                                     gtTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCgg                                                                                                             >  2:149573/1‑150 (MQ=255)
                                               cAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGt                                                                                                   >  1:424241/1‑150 (MQ=255)
                                               cAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGt                                                                                                   <  1:149573/150‑1 (MQ=255)
                                                  ggCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGcc                                                                                                >  1:55269/1‑150 (MQ=255)
                                                        gCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCg                                                                                          >  2:1052149/1‑150 (MQ=255)
                                                          ccAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCggg                                                                                        <  2:723206/150‑1 (MQ=255)
                                                          ccAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCggg                                                                                        >  1:1245794/1‑150 (MQ=255)
                                                                 gagcgcAGCGGCCAGTTGCTGATCGATTTCGCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTg                                                                                 <  1:998077/148‑1 (MQ=255)
                                                                      cAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCga                                                                                         >  2:579747/1‑136 (MQ=255)
                                                                      cAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCga                                                                                         <  1:579747/137‑2 (MQ=255)
                                                                        gCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCa                                                                          <  1:92345/150‑1 (MQ=255)
                                                                         cGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCAt                                                                         <  1:968424/150‑1 (MQ=255)
                                                                                  gCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCGGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCgg                                                                <  1:1170973/150‑1 (MQ=255)
                                                                                    tGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCggcg                                                              >  2:25341/1‑148 (MQ=255)
                                                                                       tCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgtg                                                                                                                                                 <  1:269558/64‑1 (MQ=255)
                                                                                       tCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACtgtg                                                                                                                                                 >  2:269558/1‑64 (MQ=255)
                                                                                         gATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACAGTGCGGcca                                                                                                                                           <  2:107706/68‑3 (MQ=255)
                                                                                         gATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACAGTGCGGcca                                                                                                                                           >  1:107706/1‑66 (MQ=255)
                                                                                          aTTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCgg                                                        >  2:315553/1‑150 (MQ=255)
                                                                                           tttCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGc                                                       <  2:1079375/150‑1 (MQ=255)
                                                                                           tttCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGc                                                       <  2:1079258/150‑1 (MQ=255)
                                                                                               tcCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGAt                                                   <  1:186454/150‑1 (MQ=255)
                                                                                               tcCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGAt                                                   >  2:739280/1‑150 (MQ=255)
                                                                                               tcCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGACGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGAt                                                   >  2:867878/1‑150 (MQ=255)
                                                                                                ccAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATc                                                  <  1:1052149/150‑1 (MQ=255)
                                                                                                 cAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCt                                                 >  1:1004431/1‑150 (MQ=255)
                                                                                                 cAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGATGCGCGGTGAACCGGCCGATCt                                                 >  2:328938/1‑150 (MQ=255)
                                                                                                 cAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACAGTGCGGcca                                                                                                                                           <  2:1230668/60‑3 (MQ=39)
                                                                                                 cAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACAGTGCGGcca                                                                                                                                           >  1:1230668/1‑58 (MQ=39)
                                                                                                        gAACCCACCGCCACCCGTGAAACGCGTTTCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGt                                          <  2:331652/150‑1 (MQ=255)
                                                                                                           cccACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCa                                       <  2:570849/150‑1 (MQ=255)
                                                                                                            ccACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATTGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCAc                                      <  1:1246469/150‑1 (MQ=255)
                                                                                                            ccACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCg                                                                                                              <  2:250096/78‑1 (MQ=255)
                                                                                                            ccACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCg                                                                                                              >  1:250096/1‑78 (MQ=255)
                                                                                                            ccACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCAc                                      >  2:784068/1‑150 (MQ=255)
                                                                                                             cACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACg                                     >  2:985379/1‑150 (MQ=255)
                                                                                                              aCCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGc                                    >  2:1051156/1‑150 (MQ=255)
                                                                                                                cGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGcc                                   >  2:1326932/1‑149 (MQ=255)
                                                                                                                cGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGcc                                   <  1:1326932/149‑1 (MQ=255)
                                                                                                                  ccACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGGGCACGCCCgg                                >  1:590578/1‑150 (MQ=255)
                                                                                                                       cGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGtgctg                           <  2:1051358/150‑1 (MQ=255)
                                                                                                                        gTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGtgctgc                          >  1:224251/1‑150 (MQ=255)
                                                                                                                         tGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGTCGAta                                                                           <  2:1179566/100‑2 (MQ=255)
                                                                                                                         tGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGTCGAta                                                                           >  1:1179566/1‑99 (MQ=255)
                                                                                                                         tGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCa                         >  1:1062819/1‑150 (MQ=255)
                                                                                                                               gtttGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCt                   <  2:233944/147‑1 (MQ=255)
                                                                                                                               ggCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCt                   <  2:942893/150‑1 (MQ=255)
                                                                                                                                gCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCTGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTTAAGCGTCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCtt                  <  1:1098667/150‑1 (MQ=255)
                                                                                                                                gCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCtt                  <  2:1062819/150‑1 (MQ=255)
                                                                                                                                  tGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGaa                                                            >  1:813791/1‑106 (MQ=255)
                                                                                                                                  tGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGaa                                                            <  2:813791/106‑1 (MQ=255)
                                                                                                                                   gCCGAAGTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGTACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGTGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTTCTGCAGGTGCTTGGt               <  2:540656/150‑1 (MQ=255)
                                                                                                                                   gCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGACGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGt               <  1:867878/150‑1 (MQ=255)
                                                                                                                                        ccatgCCGCACAGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAgcg          >  2:983757/5‑150 (MQ=255)
                                                                                                                                         catgCCGCACAGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCa                                              >  1:876114/4‑113 (MQ=255)
                                                                                                                                         catgCCGCACAGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCa                                              <  2:876114/110‑1 (MQ=255)
                                                                                                                                          tGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAGcgcg        >  1:880053/1‑150 (MQ=255)
                                                                                                                                           ggCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAGCGCGt       <  2:424241/150‑1 (MQ=255)
                                                                                                                                           ggCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGCGGCTTGGTCATCGCGt       <  2:590578/150‑1 (MQ=255)
                                                                                                                                            gCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGc                                                                               >  1:1240136/1‑77 (MQ=255)
                                                                                                                                            gCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGc                                                                               <  2:1240136/77‑1 (MQ=255)
                                                                                                                                            gCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCgg                                                                <  1:333558/92‑1 (MQ=255)
                                                                                                                                            gCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCgg                                                                >  2:333558/1‑92 (MQ=255)
                                                                                                                                              cGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGATGCAGGTGCTTGGTCAGCGCGTGAc    >  1:294296/1‑150 (MQ=255)
                                                                                                                                                cACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCACGGTGCCGAGTCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAGCGCGTGACGc  <  1:674255/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTGCCTGGCCGACACGGCGGTGGTGCGTGAAGTGGTGTTCGGCGAGCAAGGCATTGCCCAGGGCGGGCGCAGCGGCCAGTTGCTGATCGATTTCTCCAGCCTGGAACCCACCGCCACCCGTGAAATGGCTGCCGAACTGGCCGCACTGTGCGGCATGGCCTGGCTGGATGCACCGGTATCTGGCGGTACCCCCGGTGCCGAGGCGGGCACGCTGGCGATCATGGTGGGCGGTGAAGCGGCCGATCTGCAGCGTGCACGCCCGGTGCTGCAGGTGCTTGGTCAGCGCGTGACGC  >  NC_002947/1308686‑1308979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑