| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,364,666 | A→T | 7.5% | intergenic (+30/+119) | dctA‑I → / ← PP_1189 | C4‑dicarboxylate transport protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,364,666 | 0 | A | T | 7.5% | 163.9 / 4.3 | 53 | intergenic (+30/+119) | dctA‑I/PP_1189 | C4‑dicarboxylate transport protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/29); new base T (2/2); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
GTGGGTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCACTTCCAACACCCGGAT > NC_002947/1364519‑1364814 | gTGGGTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAgg > 1:1136962/1‑150 (MQ=255)gTGGGTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAgg > 1:853228/1‑150 (MQ=255) ggTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCg > 2:9263/1‑150 (MQ=255) gTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGc < 1:143641/150‑1 (MQ=255) tCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGAGACCaaaaaacacc > 2:791179/1‑143 (MQ=255) aGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCTTTGGTCGCCttt < 1:765385/150‑1 (MQ=255) ggAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCtttt > 1:1376452/1‑150 (MQ=255) aaaTGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTc < 1:572500/150‑1 (MQ=255) ggACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCgtgt < 1:346354/150‑1 (MQ=255) aaCGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAgc > 1:1061659/1‑150 (MQ=255) aCGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAgcg < 2:554857/150‑1 (MQ=255) cAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGa < 1:1152905/150‑1 (MQ=255) aaGCTGGCTTCGGAAATGGACTCCGGTGGTGTGTCGTTGTTCGATACCCTCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGAc < 2:726785/150‑1 (MQ=255) gCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTtgt > 2:99920/1‑150 (MQ=255) gCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTtgt < 1:408944/150‑1 (MQ=255) gAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGc < 1:791179/150‑1 (MQ=255) aaCTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGcc > 2:444032/1‑150 (MQ=255) aCTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCt < 2:1376452/150‑1 (MQ=255) cTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCtt < 2:1411432/150‑1 (MQ=255) cGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCg > 2:919340/1‑114 (MQ=255) cGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCg < 1:919340/114‑1 (MQ=255) ggtgCGCCGCTGATCGATATCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTa < 2:1149637/150‑1 (MQ=255) gcgcCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGTGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTaaaa < 1:228465/150‑1 (MQ=255) gATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGAtt > 2:640446/1‑150 (MQ=255) ccgcccgACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACt > 2:842938/1‑150 (MQ=255) cgcccgACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGTTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACtg > 1:1242642/1‑150 (MQ=255) cccgACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGTTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACtgtg < 2:1242642/150‑1 (MQ=255) cccgACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTCAAAGCTGATGCGGTTTGAcgtgg < 2:1380483/150‑5 (MQ=255) ggacgACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATAGGGCTGggggggtg > 1:952327/1‑146 (MQ=255) gacgacCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCgg < 1:640446/150‑1 (MQ=255) acCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCtt < 1:99920/150‑1 (MQ=255) cGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCcgcg < 1:444032/150‑1 (MQ=255) gTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAAttt > 2:776173/1‑105 (MQ=255) gTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAAttt < 1:776173/105‑1 (MQ=255) tGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACg > 2:1244293/1‑150 (MQ=255) tGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACg > 1:687141/1‑150 (MQ=255) gAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACgg < 2:1273225/150‑1 (MQ=255) ggCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACgg > 2:710407/1‑147 (MQ=255) ggCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACgg < 1:710407/147‑1 (MQ=255) ccGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAg < 1:9263/150‑1 (MQ=255) ccGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAg > 2:1263080/1‑150 (MQ=255) tCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGAGGGCTTGCCCGCGAACACGGGCGAAGCCCGt < 2:1136962/150‑1 (MQ=255) cGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTg < 2:1082456/150‑1 (MQ=255) agCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCa < 1:842938/150‑1 (MQ=255) tGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTaaaa > 2:455/1‑83 (MQ=255) tGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTaaaa < 1:455/83‑1 (MQ=255) gTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCa > 2:523005/1‑150 (MQ=255) ttGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAg < 2:853228/150‑1 (MQ=255) gAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTa < 1:1244293/150‑1 (MQ=255) acgaaaaaGGCGACCTTTGGTCGCCTTTCTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGaa > 2:915279/6‑125 (MQ=25) acgaaaaaGGCGACCTTTGGTCGCCTTTCTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGaa < 1:915279/120‑1 (MQ=25) aTGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGAGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCa > 2:820766/1‑150 (MQ=255) cgaaaaaGGCGACCTTTGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCAc > 2:17078/5‑150 (MQ=37) aCCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCACTTCCAACAccc < 1:820766/150‑1 (MQ=255) aaGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCACTTCCAACACCCGGAt < 2:687141/150‑1 (MQ=255) | GTGGGTCAAGGAAATGGACAACGACAAGCTGGCTTCGGAACTGGACTCCGGTGGTGCGCCGCTGATCGATACCCGCCCGACGGACGACCTGGGCGTGGCTGAAGGCCCGGCTCGTTGAGAGCTGGTTGAAATGAGAAAGGCGACCAAAGGTCGCCTTTTTCGTGTTAGCGCGGACCCTTGTGTGGGCCTTGCTCAATTTCTAAAAGCTGGCGCGATTGGACTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGAAGCCCGTGGCCCTCAGCCCAGTACCACTTCCAACACCCGGAT > NC_002947/1364519‑1364814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |