| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,365,284 | G→C | 6.6% | A67G (GCC→GGC) | PP_1189 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,365,284 | 0 | G | C | 6.6% | 188.4 / 3.8 | 61 | A67G (GCC→GGC) | PP_1189 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (26/31); new base C (2/2); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
AGGCGCCCGGGCTCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATGGCG > NC_002947/1365137‑1365421 | agGCGCCCGGGCTCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGcc < 2:184335/150‑1 (MQ=255) ggCGCCCGGGCTCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCt > 1:653084/1‑150 (MQ=255) gCTCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaaca < 1:1084360/150‑1 (MQ=255) tCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaacagg < 1:1223436/150‑1 (MQ=255) cACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaacaggaa > 2:521070/1‑150 (MQ=255) cGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaacaggaaca < 1:900224/150‑1 (MQ=255) ccTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAAcagca < 1:659093/150‑1 (MQ=255) tCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACagcagc > 1:1165055/1‑150 (MQ=255) cacggtcGTACCGCCGACTGGCCTATGCCGGTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACagcagc < 2:80735/144‑1 (MQ=255) aGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGCCCTGGAggaaa > 2:991676/1‑134 (MQ=255) aGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGCCCTGGAggaaa < 1:991676/135‑2 (MQ=255) accaccGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATgg < 1:765493/150‑1 (MQ=255) caccGACTGGCCGATGCCGTTGGGCCGGTGGCTGG‑CGCGGGTGGCGAACACACCCATGCTCTTGTTGGCGCCCGGACGCGGCGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCCGGTGGAACGAGAACAGCAGCCACACATGGcg > 2:1365563/1‑149 (MQ=255) cGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc < 2:1318438/150‑1 (MQ=255) cGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCCGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc < 1:241048/150‑1 (MQ=255) gATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAg < 1:439612/150‑1 (MQ=255) aTGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAgg < 1:521070/150‑1 (MQ=255) cGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGc < 1:1205646/100‑1 (MQ=255) cGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGc > 2:1205646/1‑100 (MQ=255) gTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc > 2:301565/1‑135 (MQ=255) gTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc < 1:301565/135‑1 (MQ=255) gTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTc > 2:845928/1‑150 (MQ=255) gTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCACCAGGCCCTGGAggaa < 1:652746/108‑1 (MQ=255) gTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCACCAGGCCCTGGAggaa > 2:652746/1‑108 (MQ=255) gggCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaacgggaa > 2:924920/1‑111 (MQ=255) gggCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaacgggaa < 1:924920/111‑1 (MQ=255) gggCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGAc > 2:465988/1‑150 (MQ=255) tcgttggtggcCGCGGGTGTGGAACCCCCCCATCCTCTTGTTGCCGCCCAGCCCCGGTGGCCGCACTTTCAGCCGCCGCTTTTCCTCCAGGGCATGTTGGAACAGGAACAGCAGCCACACATCGCTGACCTGTTCCAGGCCCTCGACcgc < 2:655161/145‑1 (MQ=255) tgg‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCtt < 1:582868/150‑1 (MQ=255) g‑cgcGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACagcagc > 1:460406/1‑105 (MQ=255) g‑cgcGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACagcagc < 2:460406/105‑1 (MQ=255) g‑cgcGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGATCTGTTCCAGGCCCTCGACCGCGTCACCTTGa < 2:872728/150‑1 (MQ=255) cGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGATGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAAcggcg > 2:822827/1‑150 (MQ=255) gAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAAcggcgg > 2:83733/1‑150 (MQ=255) acacCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaaca < 1:716048/81‑1 (MQ=255) acacCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTggaaca > 2:716048/1‑81 (MQ=255) acCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCAAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAAc > 2:324246/1‑150 (MQ=255) ccccATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAg > 2:522795/1‑150 (MQ=255) ccccATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAg > 1:1190900/1‑150 (MQ=255) ccATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCAcc > 1:1182710/1‑127 (MQ=255) ccATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCAcc < 2:1182710/127‑1 (MQ=255) ccATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGtt < 2:1165055/150‑1 (MQ=255) cATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCAAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTc < 1:324246/150‑1 (MQ=255) gCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAg < 1:522795/150‑1 (MQ=255) tctTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGa > 2:221652/1‑111 (MQ=255) tctTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGa < 1:221652/111‑1 (MQ=255) gACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAg < 2:1060437/150‑1 (MQ=255) cgGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGgcgc < 1:419739/150‑1 (MQ=255) gatctcGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTCCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCa < 1:635953/145‑1 (MQ=255) cGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGc < 2:821211/150‑1 (MQ=255) cGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCCCTTCCAGTCCCTCGCCCCGGGCACCTTGAGCGTACGGTGGTACCGGTGCGGGGTGGCCACAGGCGTCACGCCCCAGTTGc > 2:1365915/1‑150 (MQ=255) gCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAATTCGAGTACGCCACAGGCGGCGTGCACCAgagatc > 2:635953/1‑145 (MQ=255) cACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCgg < 1:465988/150‑1 (MQ=255) tttCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGAGCCAGTTGCGGCTg > 2:303235/1‑150 (MQ=255) ttCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTgg > 1:1074123/1‑150 (MQ=255) gcgGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc < 1:970807/55‑1 (MQ=255) gcgGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGAc > 2:970807/1‑55 (MQ=255) cTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATggg > 2:470119/1‑149 (MQ=255) ttttCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATGGCg > 1:553053/1‑150 (MQ=255) ctccAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATGGc > 2:241778/1‑144 (MQ=255) ctccAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATGGc < 1:241778/144‑1 (MQ=255) | AGGCGCCCGGGCTCCACGCCCTCCAGGCGTACCACCGACTGGCCGATGCCGTTGGGCCGGTGGGTGG‑CGCGGGTGGCGAACACCCCCATGCTCTTGTTGCCGCCCAGACGCGGAGGCCGCACTTTCAGGCGCGGCTTTTCCTCCAGGGCCTGGTGGAACAGGAACAGCAGCCACACATGGCTGACCTGTTCCAGGCCCTCGACCGCGTCACCTTGATCGAACGGCGGCAACAGTTCGAGTACGCCACAGGCGGCAGGCGCCAGTTGCGGCTGGCGCGGAATGGCG > NC_002947/1365137‑1365421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |