Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,380,752 C→A 13.7% intergenic (‑134/+1) PP_t67 ← / ← PP_t66 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,380,7520CA13.7% 103.7 / 4.7 42intergenic (‑134/+1)PP_t67/PP_t66tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (17/19);  new base A (4/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTTTGATTTGTCGATGTTTTTTC  >  NC_002947/1380606‑1380900
                                                                                                                                                   |                                                                                                                                                    
gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATACTTATCAGCAGGCAAAGAAAAAGGCCACTCTGTTA‑ACAGTGGCCTTTTCTTGAATCTgg                                                                                                                                                   <  1:933136/150‑1 (MQ=255)
  tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGtt                                                                                                                                                 >  1:134069/1‑150 (MQ=255)
        ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGa                                                                                                                                           >  1:787379/1‑150 (MQ=255)
               aGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAt                                                                                                                                    <  2:46054/150‑1 (MQ=255)
                    ggCGGTTTTTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                                               <  2:1071201/150‑1 (MQ=255)
                      cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGTAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAcc                                                                                                                             <  1:421724/150‑1 (MQ=255)
                      cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGACTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAcc                                                                                                                             <  1:1280157/150‑1 (MQ=255)
                                      tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGa                                                                                                                                           >  1:1083111/1‑120 (MQ=255)
                                      tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGa                                                                                                                                           <  2:1083111/120‑1 (MQ=255)
                                       ggTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGACCTGAGGACCTTCGGGTTAt                                                                                                            <  1:1118783/150‑1 (MQ=255)
                                        gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                                                 >  2:154405/1‑144 (MQ=255)
                                        gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                                                 <  1:154405/144‑1 (MQ=255)
                                          ttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCCCCGGGTTA‑ACAGTGGCCTTTTCTTGAATATGGTTGCGGGTGCCGGATTTGAACCGACGACCTTCGGGGTATAAg                                                                                                         >  1:318747/1‑150 (MQ=18)
                                          ttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                                         >  1:720168/1‑150 (MQ=255)
                                                  cAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                                                 <  2:1027766/150‑1 (MQ=255)
                                                       gcTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAc                                                                                            <  2:787379/150‑1 (MQ=255)
                                                         tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCa                                                                                          <  1:829326/150‑1 (MQ=255)
                                                                         gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAgg                                                                                        >  2:1292027/1‑136 (MQ=255)
                                                                         gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAgg                                                                                        <  1:1292027/136‑1 (MQ=255)
                                                                                       aaTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                                            >  2:634231/1‑150 (MQ=255)
                                                                                            ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                                                      >  1:522688/1‑103 (MQ=255)
                                                                                            ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                                                      <  2:522688/103‑1 (MQ=255)
                                                                                                     ggCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTa                                              <  1:634231/150‑1 (MQ=255)
                                                                                                      gCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAg                                             <  2:318747/150‑1 (MQ=255)
                                                                                                        accccgcctgtgtaaaagcagcgcggggtttgttctgacGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTg                                                                   <  1:1418501‑M2/88‑1 (MQ=255)
                                                                                                        accccgcctgtgtaaaagcagcgcggggtttgttctgacGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTg                                                                   >  2:1418501‑M2/40‑127 (MQ=255)
                                                                                                          cccgcctgtgtaaaagcagcgcggggtttgttctgacGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                                            <  1:300921‑M2/47‑1 (MQ=255)
                                                                                                          cccgcctgtgtaaaagcagcgcggggtttgttctgacGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                                            >  2:300921‑M2/38‑84 (MQ=255)
                                                                                                          aagaaaaGGCCACTGT‑TAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta                                         >  2:1208717/4‑150 (MQ=17)
                                                                                                           ccgcctgtgtaaaagcagcgcggggtttgttctgacGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGgtttg                                         >  1:918064‑M2/37‑149 (MQ=255)
                                                                                                                    cACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAgc                               >  2:824183/1‑150 (MQ=255)
                                                                                                                     aCTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                                                          >  1:75959/1‑74 (MQ=40)
                                                                                                                      cTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                                                       >  2:1257876/1‑124 (MQ=255)
                                                                                                                      cTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                                                       <  1:1257876/124‑1 (MQ=255)
                                                                                                                           ttA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTggg                                                                 <  2:1104369/109‑1 (MQ=255)
                                                                                                                           ttA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTggg                                                                 >  1:1104369/1‑109 (MQ=255)
                                                                                                                             a‑aCAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCgg                                                                                                                                      >  1:73309/1‑38 (MQ=255)
                                                                                                                                   tGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTTTGAtt                 >  2:76117/1‑150 (MQ=255)
                                                                                                                                     gCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                                            >  2:1290945/1‑57 (MQ=38)
                                                                                                                                            cTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGGGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                                            >  2:1135234/1‑98 (MQ=255)
                                                                                                                                            cTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGGGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                                            <  1:1135234/98‑1 (MQ=255)
                                                                                                                                                  tCTAGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTTTGATTTGTCGATGTTTTTTc  <  2:720168/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTTTGATTTGTCGATGTTTTTTC  >  NC_002947/1380606‑1380900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑