Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,404,894 A→T 8.5% L265Q (CTG→CAG)  PP_1228 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,404,8940AT 8.5% 139.2 / 3.4 47L265Q (CTG→CAG) PP_1228methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (27/16);  new base T (2/2);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCACGCTTTCCGGCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCAGAGCGA  >  NC_002947/1404745‑1405041
                                                                                                                                                     |                                                                                                                                                   
gcaCGCTTTCCGGCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCcagca                                                                                                                                                     >  1:766363/1‑150 (MQ=255)
 caCGCTTTCCGGCAGTTCCAGCACTACCTGCCACGAGGTTGGGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCcagcag                                                                                                                                                    <  1:1143066/150‑1 (MQ=255)
       ttCCGGCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCcggc                                                                                                                                              >  2:585836/1‑150 (MQ=255)
          cGGCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTTTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCcagcggc                                                                                                                                           >  1:215156/1‑150 (MQ=255)
           ggCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCcggcggcg                                                                                                                                          >  2:276262/1‑150 (MQ=255)
           ggCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCCACCGCCGCCGGCCTGCTGGCcggcaaca                                                                                                                                          <  1:1217836/150‑5 (MQ=255)
                ttCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGc                                                                                                                                     <  2:802948/150‑1 (MQ=255)
                   cAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGAt                                                                                                                                  <  2:1096665/150‑1 (MQ=255)
                   cAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGAt                                                                                                                                  >  1:64081/1‑150 (MQ=255)
                    aGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATg                                                                                                                                 <  1:224512/150‑1 (MQ=255)
                         tACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCAc                                                                                                                            >  2:367819/1‑150 (MQ=255)
                            cTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCAcctg                                                                                                                         >  1:225891/1‑150 (MQ=255)
                                cACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCAcctggccc                                                                                                                     >  1:204898/1‑150 (MQ=255)
                                   ggggCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCAcctggccctgg                                                                                                                  <  2:225891/150‑1 (MQ=255)
                                     ggCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACctggccctggcc                                                                                                                <  1:1199101/150‑1 (MQ=255)
                                     ggCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACctggccccggcc                                                                                                                <  2:766363/150‑1 (MQ=255)
                                              aGGGATTGGCGTGAAGGGGTGGGCGACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTGACCTGGCCCTGGCCGCCGAACAg                                                                                                       >  2:682179/1‑150 (MQ=255)
                                              aGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAg                                                                                                       >  2:270347/1‑150 (MQ=255)
                                                      gCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCgcggc                                                                                               >  2:611852/1‑150 (MQ=255)
                                                             ggggTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGtt                                                                                        >  1:1340692/1‑150 (MQ=255)
                                                                             agcagGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCt                                                                                                                               >  2:743376/1‑95 (MQ=255)
                                                                             agcagGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCt                                                                                                                               <  1:743376/95‑1 (MQ=255)
                                                                                  gCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGtt                                                                   >  1:419592/1‑150 (MQ=255)
                                                                                                  ttGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTcc                                                   >  2:935902/1‑150 (MQ=255)
                                                                                                  ttGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTcc                                                   >  1:11754/1‑150 (MQ=255)
                                                                                                  ttGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTcc                                                   >  2:936154/1‑150 (MQ=255)
                                                                                                    gTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGc                                                                                                                                <  1:1222080/71‑1 (MQ=255)
                                                                                                    gTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGc                                                                                                                                >  2:1222080/1‑71 (MQ=255)
                                                                                                    gTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAg                                                 >  1:1067934/1‑150 (MQ=255)
                                                                                                    gTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAg                                                 >  2:523175/1‑150 (MQ=255)
                                                                                                     tCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAgg                                                <  1:270347/150‑1 (MQ=255)
                                                                                                        aTGGGCTTGCCGAGCACGCTGTCGTCGTTGCTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCg                                             >  1:1107142/1‑150 (MQ=255)
                                                                                                        aTGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAAGTTGGCCAGGCCGATGTCCAGGCCg                                             >  1:296046/1‑150 (MQ=255)
                                                                                                         tGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGa                                            >  1:199885/1‑150 (MQ=255)
                                                                                                              ttGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGTGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACg                                       >  1:718896/1‑150 (MQ=255)
                                                                                                                gCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCcggcggc                                                                                                                                           >  1:73586/1‑48 (MQ=255)
                                                                                                                gCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCcggcggc                                                                                                                                           <  2:73586/48‑1 (MQ=255)
                                                                                                                  cGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGa                                   >  1:123139/1‑150 (MQ=255)
                                                                                                                             tcgtcgCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCgcgg                                                                                                >  2:133635/1‑78 (MQ=255)
                                                                                                                             tcgtcgCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCgcgg                                                                                                <  1:133635/78‑1 (MQ=255)
                                                                                                                                    gACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTAAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCa                 <  1:273034/150‑1 (MQ=255)
                                                                                                                                         ttGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAg            <  1:367819/150‑1 (MQ=255)
                                                                                                                                          tGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAgg           >  2:142064/1‑150 (MQ=255)
                                                                                                                                           gCCGGCCTGCTGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCgt                       <  2:682353/137‑1 (MQ=255)
                                                                                                                                           gCCGGCCTGCTGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCgt                       >  1:682353/1‑137 (MQ=255)
                                                                                                                                           gCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAggg          <  1:276262/150‑1 (MQ=255)
                                                                                                                                             cGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCa        <  2:123139/150‑1 (MQ=255)
                                                                                                                                               gCCTGCTGGGCGGCGGCGGCGGCGATGCTCACCTACCCCTGGCCGTCGAACAGATTGCGGCGGCCGTTAACGCTTAGTTGCTGCCGTTTGGCCAGGCCGATTTCCAGGTCGAATACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCtga      >  2:382948/1‑150 (MQ=255)
                                                                                                                                               gCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCaga      <  2:296046/150‑1 (MQ=255)
                                                                                                                                                 cagcagGCCGGCGGCGGTGGCGATGCAAGACTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCAGAGc    >  1:1107186/1‑150 (MQ=255)
                                                                                                                                                   gcagGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCAGAGCGa  <  2:419592/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCACGCTTTCCGGCAGTTCCAGCACTACCTGCCACGGGGCTGTGTCAGGGATTGGCGTGAAGGGGTGGGCCACGCGCAGCAGGCCGTCGGCTACCGACTTGTCCATGGGCTTGCCGAGCACGCTGTCGTCGCGACTGTTGCCGGCCAGCAGGCCGGCGGCGGTGGCGATGCTCACCTGGCCCTGGCCGTCGAACAGATCGCGGCGGCCGTTCACGCTCAGTTGCTGCAGGTTGGCCAGGCCGATGTCCAGGCCGACCACGCCGACTACCTTGCCGTGTTCCAGCAAGGGCAGAGCGA  >  NC_002947/1404745‑1405041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑