Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 160,311 A→G 6.0% L26L (CTT→CTC)  PP_0149 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947160,3110AG 6.0% 201.1 / 2.4 68L26L (CTT→CTC) PP_0149hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base G (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

GTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160164‑160457
                                                                                                                                                    |                                                                                                                                                  
gTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAg                                                                                                                                                  >  1:1163318/1‑150 (MQ=255)
    gcaggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGc                                                                                                                                              <  1:384630/150‑1 (MQ=255)
    gcaggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTACTGCATCGAAG‑ACCGCCGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGc                                                                                                                                              >  2:703147/1‑150 (MQ=255)
      aggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCGTGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            <  1:95750/150‑1 (MQ=255)
                tCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAg                                                                                                                                  <  2:988949/150‑1 (MQ=255)
                tCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAg                                                                                                                                  >  1:57705/1‑150 (MQ=255)
                  gCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGA‑TTAGAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCGTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggc                                                                                                                                >  2:985090/1‑150 (MQ=255)
                   cTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcg                                                                                                                               >  2:224860/1‑150 (MQ=255)
                    tCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcgg                                                                                                                              >  2:550157/1‑150 (MQ=255)
                    tCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcgg                                                                                                                              >  1:183899/1‑150 (MQ=255)
                    cccAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCACATGGTGTAGTCGAGCAGGGATCATGAGGCTTTCGTTTGTCGTGCGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcgg                                                                                                                              <  1:101184/149‑1 (MQ=255)
                           gACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGcc                                                                                                                       <  2:1010297/150‑1 (MQ=255)
                            aCCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCg                                                                                                                      >  2:827715/1‑150 (MQ=255)
                             cccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                     <  2:1298771/150‑1 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            >  2:497942/1‑126 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            <  1:497942/126‑1 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGt                                                                                                                          >  2:371443/1‑144 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGt                                                                                                                          <  1:371443/144‑1 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                    >  2:180858/1‑150 (MQ=255)
                              ccGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                    >  2:1198629/1‑150 (MQ=255)
                                gCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagca                                                                                                                  <  1:618012/150‑1 (MQ=255)
                                gCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagca                                                                                                                  <  1:827715/150‑1 (MQ=255)
                                 cACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagcag                                                                                                                 >  1:700542/1‑150 (MQ=255)
                                  aCGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTGTTGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagcagc                                                                                                                <  2:338386/150‑1 (MQ=255)
                                    gcggcgGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagcagc                                                                                                                >  2:1393793/1‑148 (MQ=255)
                                    gcggcgGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagcagc                                                                                                                <  1:1393793/148‑1 (MQ=255)
                                        cggTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAgg                                                                                                          <  1:103909/150‑1 (MQ=255)
                                        cggTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAgg                                                                                                          <  1:180858/150‑1 (MQ=255)
                                        cggTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAgg                                                                                                          <  1:224860/150‑1 (MQ=255)
                                             cAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTc                                                                                                             >  2:963599/1‑142 (MQ=255)
                                             cAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTc                                                                                                             <  1:963599/142‑1 (MQ=255)
                                              aGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAg                                                                                                    >  1:1339843/1‑150 (MQ=255)
                                                 tGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCa                                                                                                 >  2:211935/1‑150 (MQ=255)
                                                  gTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                                <  1:734867/150‑1 (MQ=255)
                                                  gTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                                <  1:550157/150‑1 (MQ=255)
                                                  gTTGC‑GTGCAGCGAGGTGGGCGCTTGCCCATGGCCTCGTCGGGCAGGGCTTGGGGGGCTTTAGTTTGTTGTGGGTCGAGCCCGGACGCCTGCCGTTCCCGCTGCAGGTCGGCTAAGGCGGTCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                                <  2:199797/150‑1 (MQ=255)
                                                      c‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAaggag                                                                                            >  1:380641/1‑150 (MQ=255)
                                                           aaGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAg                                                                                        <  1:511761/150‑1 (MQ=255)
                                                           aaGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAg                                                                                        <  2:57705/150‑1 (MQ=255)
                                                             gcgcGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgc                                                                                      >  2:27678/1‑150 (MQ=255)
                                                                  gTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgca                                                                                 <  2:183899/150‑1 (MQ=255)
                                                                   tGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgcac                                                                                <  1:1151800/150‑1 (MQ=255)
                                                                       aGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACgcgc                                                                            >  2:332820/1‑150 (MQ=255)
                                                                       aGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACgcgc                                                                            <  1:211935/150‑1 (MQ=255)
                                                                        gCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCt                                                                           >  2:1309368/1‑150 (MQ=255)
                                                                               cATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGt                                                                    >  2:469706/1‑150 (MQ=255)
                                                                               cATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGt                                                                    >  1:353428/1‑150 (MQ=255)
                                                                                 tGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  <  2:1163318/150‑1 (MQ=255)
                                                                                 tGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  <  2:1111411/150‑1 (MQ=255)
                                                                                   gCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGa                                                                <  2:1339843/150‑1 (MQ=255)
                                                                                   gCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGa                                                                <  1:703147/150‑1 (MQ=255)
                                                                                     cTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                              >  2:631268/1‑150 (MQ=255)
                                                                                                      gggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATAc                                             >  1:1218343/1‑150 (MQ=255)
                                                                                                       ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATAcc                                            <  1:170419/150‑1 (MQ=255)
                                                                                                         aGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTg                                          <  2:353428/150‑1 (MQ=255)
                                                                                                            cTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTc                                       >  2:677732/1‑150 (MQ=255)
                                                                                                                  tttGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGgc                                 >  1:208016/1‑150 (MQ=255)
                                                                                                                         tgGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                          <  1:700070/150‑1 (MQ=255)
                                                                                                                           ggTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCgcgcgc                        >  1:831681/1‑150 (MQ=255)
                                                                                                                              cGAGGACGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTc                     <  2:812837/150‑1 (MQ=255)
                                                                                                                               gAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTcc                    >  1:1098129/1‑150 (MQ=255)
                                                                                                                                aGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTctt                   <  1:985090/150‑3 (MQ=255)
                                                                                                                                    cGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTAc               >  2:200938/1‑150 (MQ=255)
                                                                                                                                      gACGCCTGCAGCTCAAGCTGCAGGTCGGCAAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc             >  1:827662/1‑150 (MQ=255)
                                                                                                                                           cTGCAGCTTGAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctcg        >  1:766542/1‑150 (MQ=255)
                                                                                                                                           cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCg                   >  2:651636/1‑139 (MQ=255)
                                                                                                                                           cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCg                   <  1:651636/139‑1 (MQ=255)
                                                                                                                                                 cTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAgg  >  1:1353024/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGC‑TTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160164‑160457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑