Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,475,032 T→C 10.8% E259G (GAG→GGG)  PP_1289 ← DUF328 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,475,0320TC10.8% 110.5 / 2.3 44E259G (GAG→GGG) PP_1289DUF328 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (21/18);  new base C (2/3);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCCGCGGGCTTTC  >  NC_002947/1474889‑1475181
                                                                                                                                                 |                                                                                                                                                     
gATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGTCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCgg                                                                                                                                                 <  1:726364/150‑1 (MQ=255)
aaTAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGAGCAGGGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCgg                                                                                                                                                 <  2:1342113/149‑1 (MQ=255)
   aaaGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGt                                                                                                                                              >  1:1422210/1‑150 (MQ=255)
     aGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGa                                                                                                                                            >  2:164642/1‑150 (MQ=255)
        gAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATcg                                                                                                                                         <  2:1341166/150‑1 (MQ=255)
            tGCGCCGGATTTTTGGCGTCGTATTCGCGGCGTGATAGCGGTTTGCAGCGTGCCCAGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCCCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCa                                                                                                                                     <  1:497581/150‑1 (MQ=255)
            tGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCa                                                                                                                                     <  1:164642/150‑1 (MQ=255)
                 gttaTTTTGGCCGTCGGATTCGTCGCGTGATGGAGGTTTGCACCATGCCCTGTTGCAGGGCAGACGCGGAACCCGGCGGCTCACATCTTCGCGGCTTCGGGCGCGAAG‑AGGGC‑GTGACAGCCTCACCCCGCGGGGTGATCGCGCAGGAac                                                                                                                                <  2:323362/147‑1 (MQ=255)
                     tttttGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCa                                                                                                                            >  2:837493/1‑150 (MQ=255)
                           gCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGt                                                                                                                      >  1:1308050/1‑150 (MQ=255)
                            cGTAGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTc                                                                                                                     <  1:759890/150‑1 (MQ=255)
                                ggATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGc                                                                                                                 >  1:853332/1‑150 (MQ=255)
                                  aTTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCtt                                                                                                               >  2:389918/1‑150 (MQ=255)
                                     ggTGGCGTGAGGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGa                                                                                                            <  2:1370119/149‑1 (MQ=255)
                                      gTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGAc                                                                                                           >  1:45522/1‑150 (MQ=255)
                                            tGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCg                                                                                                     <  1:923472/150‑1 (MQ=255)
                                              aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCgcgc                                                                                                                                      >  1:614551/1‑115 (MQ=255)
                                              aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGATGATCgcgc                                                                                                                                      <  2:614551/115‑1 (MQ=255)
                                               tGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCa                                                                                                  >  2:1191420/1‑150 (MQ=255)
                                               tGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCGCTTGGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCa                                                                                                  >  2:485928/1‑150 (MQ=255)
                                                       tGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagta                                                                                          <  2:1422210/150‑1 (MQ=255)
                                                                  cTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACCCCGCgga                                                                                                                                                <  2:178105/85‑2 (MQ=255)
                                                                  cTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACCCCGCgga                                                                                                                                                <  2:888787/85‑2 (MQ=255)
                                                                  cTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACCCCGCgga                                                                                                                                                >  1:888787/1‑84 (MQ=255)
                                                                  cTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACCCCGCgga                                                                                                                                                >  1:178105/1‑84 (MQ=255)
                                                                           cgatctggCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACt                                                                      <  2:247837/144‑1 (MQ=255)
                                                                                  gCGGATCACGGCGGCTGACCTCTTCGAGGCTTCAAACGTGCCGCCGAACACCGACA‑GC‑CACTCCGCGGGGCGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTgagatcg                                                               >  1:247837/1‑144 (MQ=255)
                                                                                     gATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTg                                                            >  2:750296/1‑150 (MQ=255)
                                                                                       tCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAccc                                                                                    >  2:102001/1‑124 (MQ=255)
                                                                                       tCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAccc                                                                                    <  1:102001/124‑1 (MQ=255)
                                                                                         aCGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTc                                                                                                                     <  2:134915/89‑1 (MQ=255)
                                                                                         aCGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTc                                                                                                                     >  1:134915/1‑89 (MQ=255)
                                                                                           ggcggcTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCgg                                                      <  2:1308050/150‑1 (MQ=255)
                                                                                                    ccTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtag                                                                                         <  1:478888/106‑1 (MQ=255)
                                                                                                    ccTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtag                                                                                         >  2:478888/1‑106 (MQ=255)
                                                                                                     ctctTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATg                                            >  2:793596/1‑150 (MQ=255)
                                                                                                        ttCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgcgc                                         >  2:670502/1‑150 (MQ=255)
                                                                                                                       gcgAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAg                          <  1:793596/150‑1 (MQ=255)
                                                                                                                                ggC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtagt                                                                                        >  2:1142707/1‑80 (MQ=255)
                                                                                                                                ggC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtagt                                                                                        <  1:1142707/80‑1 (MQ=255)
                                                                                                                                      gACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGc             >  1:1303693/1‑150 (MQ=255)
                                                                                                                                         aGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCcgc          >  1:1089508/1‑150 (MQ=255)
                                                                                                                                         aGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCcgc          <  1:670502/150‑1 (MQ=255)
                                                                                                                                                cTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCCGCGGGCttt   >  1:979377/1‑150 (MQ=255)
                                                                                                                                                 tCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCCTCGGGCTTTc  >  1:575143/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAG‑AGGGC‑GTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGATGACGAAGCGGCTCATCATGCCGCGGGCTTTC  >  NC_002947/1474889‑1475181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑