Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,489,374 C→T 13.3% intergenic (+166/‑37) ybgI → / → cysD metal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,489,3740CT13.3% 108.5 / 8.9 45intergenic (+166/‑37)ybgI/cysDmetal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2
Reads supporting (aligned to +/- strand):  ref base C (19/20);  new base T (2/4);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TTCGCGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCG  >  NC_002947/1489226‑1489516
                                                                                                                                                    |                                                                                                                                              
ttCGCGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCg                                                                                                                                               >  1:159117/1‑150 (MQ=255)
    cgGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAgg                                                                                                                                           <  1:905816/150‑1 (MQ=255)
      ggCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGcc                                                                                                                                         >  2:287253/1‑150 (MQ=255)
          tCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATACAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgt                                                                                                                                     <  1:1323330/150‑3 (MQ=255)
            aTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                   <  1:1139367/150‑5 (MQ=255)
            aTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                   <  2:1393187/150‑5 (MQ=255)
                    gggTTCACCCGCGAAGAGGCCGGCCTTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGt                                                                                                                           <  2:5841/150‑1 (MQ=255)
                            ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTGGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                   <  1:859037/150‑1 (MQ=255)
                            ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTGGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                   >  2:859037/1‑150 (MQ=255)
                            ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                   >  1:283533/1‑150 (MQ=255)
                            ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                   >  2:451744/1‑150 (MQ=255)
                                gAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTa                                                                                                               <  1:1021096/150‑1 (MQ=255)
                                   gagGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGcc                                                                                                            >  2:363462/1‑150 (MQ=255)
                                    agGCCGGCCCTGCCAGCCCCAACCCCAAGTCATATCGTTAGACTTTTTCGCTCTATCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCa                                                                                                           <  1:653950/150‑1 (MQ=255)
                                    agGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGt                                                                                                                                       >  1:337145/1‑122 (MQ=255)
                                    agGCCGGCCCTGCCAGCACAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGt                                                                                                                                       <  2:337145/122‑1 (MQ=255)
                                         ggccCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                      <  1:1024474/150‑1 (MQ=255)
                                               gCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACaaa                                                                                                <  1:134821/150‑1 (MQ=255)
                                                ccAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAAc                                                                                               <  1:305470/150‑1 (MQ=255)
                                                          cccccAGTCCTATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACtt                                                                                     <  2:164155/150‑1 (MQ=255)
                                                          cccccAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACtt                                                                                     <  2:159117/150‑1 (MQ=255)
                                                           ccccAGTCATATCGTTAGACTTTTTCTATTTATCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTTGACAAACTGACGCACTTg                                                                                    >  2:721660/1‑150 (MQ=255)
                                                                    tatCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTg                                                                           <  2:883130/150‑1 (MQ=255)
                                                                     atCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGt                                                                                                                    >  2:74503/1‑108 (MQ=255)
                                                                     atCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGt                                                                                                                    <  1:74503/108‑1 (MQ=255)
                                                                             aCTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGgag                                                                  <  1:451744/150‑1 (MQ=255)
                                                                                       tCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCcaca                                                        <  2:283533/150‑1 (MQ=255)
                                                                                                  tcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAg                                             <  1:287253/150‑1 (MQ=255)
                                                                                                  tcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAg                                             >  2:352372/1‑150 (MQ=255)
                                                                                                     tAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                      <  1:1082580/90‑1 (MQ=255)
                                                                                                     tAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                      >  2:1082580/1‑90 (MQ=255)
                                                                                                                 gctgctGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTgg                                                                          >  1:77314/1‑106 (MQ=255)
                                                                                                                 gctgctGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTgg                                                                          <  2:77314/106‑1 (MQ=255)
                                                                                                                      tgtgATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAACATCCACATCATCCGCGAGGTGGCCGCCGAGTTGGACaa                         >  2:566947/1‑150 (MQ=255)
                                                                                                                        tgATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAAcc                       >  1:160603/1‑150 (MQ=255)
                                                                                                                           tAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCgg                    <  1:1307151/150‑1 (MQ=255)
                                                                                                                                 ggCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGc              <  1:363462/150‑1 (MQ=255)
                                                                                                                                  gctcgctcGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCt             >  1:232459/1‑150 (MQ=255)
                                                                                                                                    tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt           >  2:1016449/1‑150 (MQ=255)
                                                                                                                                    tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt           >  2:612182/1‑150 (MQ=255)
                                                                                                                                    tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt           >  1:1280693/1‑150 (MQ=255)
                                                                                                                                      gctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTAc         >  1:400191/1‑150 (MQ=255)
                                                                                                                                            acacGGCCCGCAGGCCGTCCATAAGATCGTTTTTCATGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATcc                                                  >  1:696373/1‑103 (MQ=255)
                                                                                                                                            acacGGCCCGCAGGCCGTCCATAAGATCGTTTTTCATGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATcc                                                  <  2:696373/103‑1 (MQ=255)
                                                                                                                                             gacGGCCTGCGGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGCACTCGATCg  >  1:830711/2‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TTCGCGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCG  >  NC_002947/1489226‑1489516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: