Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,489,377	A→G	13.3%	intergenic (+169/‑34)	ybgI → / → cysD	metal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,489,377	0	A	G	13.3%	109.2 / 8.3	45	intergenic (+169/‑34)	ybgI/cysD	metal‑binding hydrolase/oxidase/sulfate adenylyltransferase subunit 2
Reads supporting (aligned to +/- strand):  ref base A (18/21);  new base G (2/4);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

CGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAC  >  NC_002947/1489230‑1489524                                                                                                                                                    |                                                                                                                                                    cgGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAgg                                                                                                                                                   <  1:905816/150‑1 (MQ=255)   ggCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGcc                                                                                                                                                 >  2:287253/1‑150 (MQ=255)       tCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATACAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgt                                                                                                                                             <  1:1323330/150‑3 (MQ=255)         aTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                           <  1:1139367/150‑5 (MQ=255)         aTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGtgttc                                                                                                                                           <  2:1393187/150‑5 (MQ=255)                 gggTTCACCCGCGAAGAGGCCGGCCTTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGt                                                                                                                                   <  2:5841/150‑1 (MQ=255)                         ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTGGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                           >  2:859037/1‑150 (MQ=255)                         ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTGGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                           <  1:859037/150‑1 (MQ=255)                         ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                           >  2:451744/1‑150 (MQ=255)                         ccGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTg                                                                                                                           >  1:283533/1‑150 (MQ=255)                             gAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTa                                                                                                                       <  1:1021096/150‑1 (MQ=255)                                gagGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGcc                                                                                                                    >  2:363462/1‑150 (MQ=255)                                 agGCCGGCCCTGCCAGCCCCAACCCCAAGTCATATCGTTAGACTTTTTCGCTCTATCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCa                                                                                                                   <  1:653950/150‑1 (MQ=255)                                 agGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGt                                                                                                                                               >  1:337145/1‑122 (MQ=255)                                 agGCCGGCCCTGCCAGCACAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCTGCGGGCCGt                                                                                                                                               <  2:337145/122‑1 (MQ=255)                                      ggccCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                              <  1:1024474/150‑1 (MQ=255)                                            gCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACaaa                                                                                                        <  1:134821/150‑1 (MQ=255)                                             ccAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAAc                                                                                                       <  1:305470/150‑1 (MQ=255)                                                       cccccAGTCCTATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACtt                                                                                             <  2:164155/150‑1 (MQ=255)                                                       cccccAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACtt                                                                                             <  2:159117/150‑1 (MQ=255)                                                        ccccAGTCATATCGTTAGACTTTTTCTATTTATCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTTGACAAACTGACGCACTTg                                                                                            >  2:721660/1‑150 (MQ=255)                                                                 tatCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTg                                                                                   <  2:883130/150‑1 (MQ=255)                                                                  atCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGt                                                                                                                            >  2:74503/1‑108 (MQ=255)                                                                  atCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGt                                                                                                                            <  1:74503/108‑1 (MQ=255)                                                                          aCTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGgag                                                                          <  1:451744/150‑1 (MQ=255)                                                                                    tCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCcaca                                                                <  2:283533/150‑1 (MQ=255)                                                                                               tcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAg                                                     >  2:352372/1‑150 (MQ=255)                                                                                               tcctAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAg                                                     <  1:287253/150‑1 (MQ=255)                                                                                                  tAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                              >  2:1082580/1‑90 (MQ=255)                                                                                                  tAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTc                                                                                                              <  1:1082580/90‑1 (MQ=255)                                                                                                              gctgctGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTgg                                                                                  <  2:77314/106‑1 (MQ=255)                                                                                                              gctgctGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTgg                                                                                  >  1:77314/1‑106 (MQ=255)                                                                                                                   tgtgATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAACATCCACATCATCCGCGAGGTGGCCGCCGAGTTGGACaa                                 >  2:566947/1‑150 (MQ=255)                                                                                                                     tgATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAAcc                               >  1:160603/1‑150 (MQ=255)                                                                                                                        tAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCgg                            <  1:1307151/150‑1 (MQ=255)                                                                                                                              ggCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGc                      <  1:363462/150‑1 (MQ=255)                                                                                                                               gctcgctcGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCt                     >  1:232459/1‑150 (MQ=255)                                                                                                                                 tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt                   >  1:1280693/1‑150 (MQ=255)                                                                                                                                 tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt                   >  2:612182/1‑150 (MQ=255)                                                                                                                                 tcgctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGt                   >  2:1016449/1‑150 (MQ=255)                                                                                                                                   gctcgAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTAc                 >  1:400191/1‑150 (MQ=255)                                                                                                                                         acacGGCCCGCAGGCCGTCCATAAGATCGTTTTTCATGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATcc                                                          <  2:696373/103‑1 (MQ=255)                                                                                                                                         acacGGCCCGCAGGCCGTCCATAAGATCGTTTTTCATGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATcc                                                          >  1:696373/1‑103 (MQ=255)                                                                                                                                          gacGGCCTGCGGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGCACTCGATCg          >  1:830711/2‑150 (MQ=255)                                                                                                                                                  gCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAc  <  2:232459/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGGCCTCATAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCTGCCAGCCCAAACCCCCAGTCATATCGTTAGACTTTTTCGATCTAGCCAGCCTCCTAAATAGAAGCAGCTGCTGTGATAAAGTGGCTCGCTCGAACACGGCCCGCAGGCCGTCCATAAGATCGTTTTTCGTGAGTAGCCATGGTCGACAAACTGACGCACTTGAAACAGCTGGAGGCGGAGAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGACAACCCGGTGATGCTGTACTCGATCGGCAAGGAC  >  NC_002947/1489230‑1489524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑