Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,506,939 T→C 8.5% intergenic (+51/‑66) petC → / → sspA ubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,506,9390TC 8.5% 127.8 / 2.4 47intergenic (+51/‑66)petC/sspAubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base C (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

AAAGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAG  >  NC_002947/1506791‑1507088
                                                                                                                                                    |                                                                                                                                                     
aaaGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCTCCCGGGGTGGCCTAGCAGTAtt                                                                                                                                                      <  1:754713/150‑1 (MQ=255)
       gcgcATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTGGCAATACTGCTAGGc                                                                                                                                               <  2:565446/150‑1 (MQ=255)
       gcgcATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                               <  1:970507/150‑1 (MQ=255)
       gcgcATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                               <  2:553969/150‑1 (MQ=255)
                   cTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGgcgc                                                                                                                                   >  1:1316173/1‑150 (MQ=255)
                   cTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGgcgc                                                                                                                                   >  2:258643/1‑150 (MQ=255)
                     aTGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGt                                                                                                                                 <  2:1402326/150‑1 (MQ=255)
                           tGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCAt                                                                                                                           <  2:1316173/150‑1 (MQ=255)
                                   cTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGtt                                                                                                                   >  1:381873/1‑150 (MQ=255)
                                   cTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCACGCCACCCCGGGCGCGTTTTCATTTAAAGtt                                                                                                                   <  2:396157/150‑1 (MQ=255)
                                    tGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTg                                                                                                                  >  2:1285857/1‑150 (MQ=255)
                                      gCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGca                                                                                                                <  1:1285857/150‑1 (MQ=255)
                                            ttcttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCa                                                                                                          >  2:1238537/1‑150 (MQ=255)
                                                tcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCAtt                                                                                                                          <  2:981999/130‑1 (MQ=255)
                                                tcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCAtt                                                                                                                          >  1:981999/1‑130 (MQ=255)
                                                tcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCg                                                                                                      <  1:491989/150‑1 (MQ=255)
                                                             cTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGc                                                                                         <  2:795473/150‑1 (MQ=255)
                                                                    aaGCGTGAATACTGGAAGGACGTGCACTGATCACTCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATgg                                                                                  >  2:934215/1‑150 (MQ=255)
                                                                    aaGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATgg                                                                                  <  2:381873/150‑1 (MQ=255)
                                                                      gCGTGAATACTGGAAGGACGTGCACTTATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGTGGTGGGCTAGCAGTATTGCCAGGCCCCCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGgc                                                                                <  2:385632/150‑1 (MQ=255)
                                                                           aaTACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCTACCCCGGGCGCGTTTTCATTTAAAGTTGCACAGGCATCCGTGCGAGGAGCCGCTACATGGGCGcaac                                                                           >  2:25311/1‑150 (MQ=255)
                                                                           aaTACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaac                                                                           <  1:1238537/150‑1 (MQ=255)
                                                                                  gAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAgg                                                                    >  1:978684/1‑150 (MQ=255)
                                                                                   aaGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGt                                                                   <  1:510801/150‑1 (MQ=255)
                                                                                     ggACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTc                                                                                                                             <  1:1329366/90‑1 (MQ=255)
                                                                                     ggACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTc                                                                                                                             >  2:1329366/1‑90 (MQ=255)
                                                                                      gACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGc                                                                                                   >  2:1362177/1‑115 (MQ=255)
                                                                                      gACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGc                                                                                                   <  1:1362177/115‑1 (MQ=255)
                                                                                        cGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGcc                                                              <  2:978684/150‑1 (MQ=255)
                                                                                          tGCACTGATCACGAAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTg                                                            >  2:1155625/1‑150 (MQ=255)
                                                                                               tGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCAt                                                                                                                           <  2:575441/82‑1 (MQ=255)
                                                                                               tGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCAt                                                                                                                           >  1:575441/1‑82 (MQ=255)
                                                                                               tGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCg                                                                                          <  1:24507/115‑1 (MQ=255)
                                                                                               tGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCg                                                                                          >  2:24507/1‑115 (MQ=255)
                                                                                                 aTCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCAcc                                                                                                                                           <  2:754801/64‑1 (MQ=255)
                                                                                                 aTCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCAcc                                                                                                                                           >  1:754801/1‑64 (MQ=255)
                                                                                                 aTCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTcc                                                     <  1:258643/150‑1 (MQ=255)
                                                                                                        agtagtTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTAtt                                                       >  1:1308261/1‑141 (MQ=255)
                                                                                                        agtagtTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTAtt                                                       <  2:1308261/141‑1 (MQ=255)
                                                                                                                                cccGGGGTGGCCTAGCAGTCTTGCCAGTCCATCCCGGTCGCGTTTTCATTTAAAGTTGCACAAGCATCCGGTCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTc                      <  1:25311/150‑1 (MQ=255)
                                                                                                                                  cGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTTAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTCTTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGc                    <  1:1155625/150‑1 (MQ=255)
                                                                                                                                   ggggTGGCCTGGCAATACTGCTAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGcc                   >  2:1127082/1‑150 (MQ=255)
                                                                                                                                        ggCCTGGCAATACTGCTAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTTGGTTTGCGCAACCAACAGGTTAGCCTGATATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGAtt              >  1:317753/1‑150 (MQ=37)
                                                                                                                                         gCCTGGCAATACTGCTAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTc                                                      <  1:383300/109‑1 (MQ=38)
                                                                                                                                         gCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGc                                                                                                   <  2:1000056/64‑1 (MQ=255)
                                                                                                                                         gCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGc                                                                                                   >  1:1000056/1‑64 (MQ=255)
                                                                                                                                           cTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTg           >  2:464178/1‑150 (MQ=255)
                                                                                                                                            tAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTgg          >  2:567546/1‑150 (MQ=255)
                                                                                                                                                    ttGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAg  >  1:1371918/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAG  >  NC_002947/1506791‑1507088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑