| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,508,087 | G→A | 7.8% | intergenic (+17/+45) | sspB → / ← PP_1322 | ClpXP protease specificity‑enhancing factor/lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,508,087 | 0 | G | A | 7.8% | 142.7 / 2.5 | 51 | intergenic (+17/+45) | sspB/PP_1322 | ClpXP protease specificity‑enhancing factor/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (22/25); new base A (2/2); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
GCTGGAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGCCCAG > NC_002947/1507938‑1508233 | gCTGGAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCg > 2:1409930/1‑150 (MQ=255) ggAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCggat < 1:144650/150‑1 (MQ=255) cTTCGATGGATGGCGACGAGCTGGACGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCAAAGCCTGAAACTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGcc < 1:930499/150‑1 (MQ=255) cTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGcc < 1:226746/150‑1 (MQ=255) ttCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGCTGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGCTCGCC‑t > 2:360537/1‑150 (MQ=255) gatggatgGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑tttt > 2:901045/1‑150 (MQ=255) tggatggGGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑tttttt < 2:1018257/150‑1 (MQ=255) gacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGCGAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGcc > 1:180307/1‑150 (MQ=255) cTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCa > 2:1199288/1‑150 (MQ=255) cTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCa > 2:319401/1‑150 (MQ=255) gatgatGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑ttt < 2:33931/124‑1 (MQ=255) gatgatGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑ttt > 1:33931/1‑124 (MQ=255) gatgatGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCAc > 1:516930/1‑150 (MQ=255) gatgatGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGACGGCCAATCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCAc > 2:343936/1‑150 (MQ=255) tgatgTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTg > 1:476929/1‑150 (MQ=255) ggtgCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGt < 2:888976/149‑1 (MQ=255) aGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATc < 2:476929/150‑1 (MQ=255) gCCGGATGACGATGGCCCTCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCg < 2:180307/150‑1 (MQ=255) gCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCg > 2:77717/1‑150 (MQ=255) cGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGAt < 1:1409930/150‑1 (MQ=255) cGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATCGATCGGC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGAt < 2:566188/150‑1 (MQ=255) aCGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTc < 1:901045/150‑1 (MQ=255) cGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACCCAGAAAGGCGATCCGGATGGCTCGCC‑TTATTTGGTTGGCCTTTCCAGGCAATCACTGAGTCAATCGATGTACTCg > 1:673548/1‑150 (MQ=255) gATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGa > 1:1068879/1‑150 (MQ=255) cccGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGc < 1:463206/150‑1 (MQ=255) ccGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATc > 2:688162/1‑133 (MQ=255) ccGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATc < 1:688162/133‑1 (MQ=255) ccGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCt > 1:1116737/1‑150 (MQ=255) ccGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCt < 2:1068879/150‑1 (MQ=255) ccAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGa < 1:485259/150‑1 (MQ=255) cAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGAc > 1:23761/1‑150 (MQ=255) gACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGa > 2:1160254/1‑150 (MQ=255) gATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCATCCGGATCGCC‑ttttttt > 1:1401008/1‑91 (MQ=37) gATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCATCCGGATCGCC‑ttttttt < 2:1401008/92‑2 (MQ=37) ccgcGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGaaa > 2:693891/1‑150 (MQ=255) gTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAACGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAcc < 2:261346/150‑1 (MQ=255) gTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAcc < 1:1160254/150‑1 (MQ=255) tCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAccc < 2:23761/150‑1 (MQ=255) ccGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCt < 1:18681/150‑1 (MQ=255) tcttgaccTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCG‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCa < 1:360537/144‑1 (MQ=255) aGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTgg < 2:95948/150‑1 (MQ=255) tCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGcttc < 2:673548/150‑1 (MQ=255) gTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGCAacac < 2:708632/115‑1 (MQ=255) gTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAacac > 1:708632/1‑115 (MQ=255) aaacaaaaaaGGCGATCCATCCGGATCGCCTTTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGa > 2:1345391/5‑90 (MQ=18) aaacaaaaaaGGCGATCCATCCGGATCGCCTTTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGa < 1:1345391/86‑1 (MQ=18) aCAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACgg < 1:1199288/150‑1 (MQ=255) aaaaaaaGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGt < 1:343936/150‑1 (MQ=255) aaaaaaaGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGt > 2:1239411/1‑150 (MQ=255) aGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAg > 2:282934/1‑150 (MQ=255) cGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGcc < 2:516930/150‑1 (MQ=255) gATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGccc > 1:630284/1‑150 (MQ=255) tCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGCCcag > 1:475680/1‑150 (MQ=255) ggatggatCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAg > 1:610741/1‑141 (MQ=255) ggatggatCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAg < 2:610741/141‑1 (MQ=255) | GCTGGAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGATCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGCCCAG > NC_002947/1507938‑1508233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |