Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,526,613	G→T	7.2%	C253F (TGC→TTC)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,526,613	0	G	T	7.2%	218.3 / 3.7	70	C253F (TGC→TTC)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base G (28/36);  new base T (3/2);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACT  >  NC_002947/1526475‑1526759                                                                                                                                            |                                                                                                                                                   cgccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGTTACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTg                                                                                                                                         <  2:625875/150‑1 (MQ=255)   ccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGc                                                                                                                                       >  2:363564/1‑150 (MQ=255)          cccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAtt                                                                                                                                          <  2:142552/140‑1 (MQ=255)          cccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAtt                                                                                                                                          >  1:142552/1‑140 (MQ=255)           ccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCAACCTGACCGCGCCCAGCCTTCGATGCCAATGGCATCGCAg                                                                                                                                <  2:118290/150‑1 (MQ=255)                    gccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCg                                                                                                                      >  2:1397231/1‑150 (MQ=255)                    gccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCg                                                                                                                      >  1:702590/1‑150 (MQ=255)                     ccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGAGCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCgg                                                                                                                     <  2:702590/150‑1 (MQ=255)                       gcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                   <  2:1177650/150‑1 (MQ=255)                         aCGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTgg                                                                                                                 >  1:1201423/1‑150 (MQ=255)                         aCGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCCCAGCCTTCGATGCCAATg                                                                                                                                         >  1:1193583/1‑127 (MQ=255)                         aCGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCCCAGCCTTCGATGCCAATg                                                                                                                                         <  2:1193583/127‑1 (MQ=255)                              ttCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTg                                                                                                            >  2:726331/1‑150 (MQ=255)                              ttCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTg                                                                                                            <  2:261927/150‑1 (MQ=255)                               tCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGa                                                                                                           <  1:559646/150‑1 (MQ=255)                               tCTACGACTATGACGCCAAGTACATCGGCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGa                                                                                                           <  2:1078594/150‑1 (MQ=255)                                 tACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATg                                                                                                         <  2:92459/150‑1 (MQ=255)                                  aCGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGc                                                                                                        <  2:909459/150‑1 (MQ=255)                                   cGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTTTATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCa                                                                                                       <  2:1404914/150‑1 (MQ=255)                                    gACTATTACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAg                                                                                                      >  2:1158806/1‑150 (MQ=255)                                    gACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAg                                                                                                      <  1:1397231/150‑1 (MQ=255)                                             gCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAg                                                                                             <  2:1201423/150‑1 (MQ=255)                                                 aGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGcc                                                                                         <  1:745148/150‑1 (MQ=255)                                                     cATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCa                                                                                                       >  2:783534/1‑132 (MQ=255)                                                     cATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCa                                                                                                       <  1:783534/132‑1 (MQ=255)                                                        cGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTg                                                                                  <  1:726331/150‑1 (MQ=255)                                                         gTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑TCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTgg                                                                                 <  2:1405905/150‑1 (MQ=255)                                                          tCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAATGGCATCgc                                                                                                                                  >  1:1018669/1‑99 (MQ=255)                                                          tCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAATGGCATCgc                                                                                                                                  <  2:1018669/100‑2 (MQ=255)                                                             aCGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgctgc                                                                             >  2:331261/1‑150 (MQ=255)                                                               gATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTc                                                                           <  1:874277/150‑1 (MQ=255)                                                                 tACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTggg                                                                                                                          >  2:896726/1‑101 (MQ=255)                                                                 tACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTggg                                                                                                                          <  1:896726/101‑1 (MQ=255)                                                                  aCCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                        >  2:109068/1‑150 (MQ=255)                                                                  aCCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                        <  1:331261/150‑1 (MQ=255)                                                                   cccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGAc                                                                                                   <  1:696394/122‑1 (MQ=255)                                                                   cccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGAc                                                                                                   >  2:696394/1‑122 (MQ=255)                                                                    ccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGc                                                                                                                                       >  1:102823/1‑85 (MQ=255)                                                                    ccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGc                                                                                                                                       <  2:102823/85‑1 (MQ=255)                                                                     cAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                        <  1:351147/147‑1 (MQ=255)                                                                     cAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                        >  2:351147/1‑147 (MQ=255)                                                                          ccGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAacac                                                                <  1:363564/150‑1 (MQ=255)                                                                              aTCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCa                                                            >  2:552691/1‑150 (MQ=255)                                                                                 ccGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCa                                                         >  1:1218720/1‑150 (MQ=255)                                                                                 ccGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAATGGCATCgcag                                                                                                                                >  1:1347923/1‑76 (MQ=255)                                                                                 ccGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAATGGCATCGCAg                                                                                                                                <  2:1347923/79‑1 (MQ=255)                                                                                     gCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCa                                                     <  1:270157/150‑1 (MQ=255)                                                                                        ggCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAggg                                                                                           >  2:1196747/1‑109 (MQ=255)                                                                                        ggCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAggg                                                                                           <  1:1196747/109‑1 (MQ=255)                                                                                         gCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGAc                                                 <  1:109068/150‑1 (MQ=255)                                                                                           tGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGaa                                                                                                                                 >  2:367638/1‑68 (MQ=255)                                                                                           tGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGaa                                                                                                                                 <  1:367638/68‑1 (MQ=255)                                                                                            ggATAGCGTCAAGGAACAGGAATTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGAGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGa                                              <  1:172497/150‑1 (MQ=255)                                                                                                 gCGTCAGGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATa                                         <  2:531239/150‑1 (MQ=255)                                                                                                   gTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGc                                       >  1:1149190/1‑150 (MQ=255)                                                                                                          aacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGc                                >  1:593568/1‑150 (MQ=255)                                                                                                           acaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGcc                               >  2:66283/1‑150 (MQ=255)                                                                                                             aggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCa                             >  2:1225727/1‑150 (MQ=255)                                                                                                               gaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTg                                                                                  >  2:517011/1‑95 (MQ=255)                                                                                                               gaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTg                                                                                  <  1:517011/95‑1 (MQ=255)                                                                                                                    gATCCACCAGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATggcggc                      <  2:523136/150‑1 (MQ=255)                                                                                                                     aTCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGcc                     >  2:273647/1‑150 (MQ=255)                                                                                                                         aCCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGAt                                             >  1:1346285/1‑122 (MQ=255)                                                                                                                         aCCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGAt                                             <  2:1346285/122‑1 (MQ=255)                                                                                                                             gACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAg                                                                       <  2:400418/92‑1 (MQ=255)                                                                                                                             gACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAg                                                                       >  1:400418/1‑92 (MQ=255)                                                                                                                                    cgc‑gcCTCCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGAACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTg      <  1:35785/150‑1 (MQ=255)                                                                                                                                      cagcCTTCGATGCCAATGGCATCGCAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTgg     >  1:209889/3‑150 (MQ=255)                                                                                                                                      c‑gcCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGa    <  2:593568/150‑1 (MQ=255)                                                                                                                                         ccTTCGATGCCAATGGCATCGCAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACt  >  1:292512/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                                   CGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACT  >  NC_002947/1526475‑1526759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑