| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,543,049 | G→T | 8.8% | G263W (GGG→TGG) | PP_1353 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,543,049 | 0 | G | T | 8.8% | 133.8 / 2.5 | 46 | G263W (GGG→TGG) | PP_1353 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (19/22); new base T (2/2); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
AAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTCACAGCAATTTCCAGCCATTTCTGG > NC_002947/1542906‑1543198 | aaGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCg > 2:477481/1‑150 (MQ=255) aCCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACa < 2:1081701/150‑1 (MQ=255) cGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTc < 1:611180/150‑1 (MQ=255) aGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGc > 2:216313/1‑150 (MQ=255) gTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCgg < 1:216313/150‑1 (MQ=255) gACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGCTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTcg > 1:1254776/1‑150 (MQ=255) aCGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTcgc < 1:1076943/150‑1 (MQ=255) tGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGCTTCACACTTGCGCCA‑GCGCAAATGGAACAAGTATGCGCGTGACCAATTACAGGCAGCACAAATCTACATGCCTTTTCCGCAGCGGGTTATTCGCgtgg < 1:1391493/150‑1 (MQ=255) gtCGATCACTGTAGCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTGAACGAGTATGTGCGTGACCGGTTGAAGGGCGAAGGGATCGACATGCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGAc < 2:328009/149‑1 (MQ=255) cTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAATGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGAc > 2:527841/1‑150 (MQ=255) tCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCaa < 1:477481/150‑1 (MQ=255) cGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCa < 2:1236773/150‑1 (MQ=255) gcTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCcgcg > 1:272753/1‑150 (MQ=255) ggACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATg > 2:433335/1‑139 (MQ=255) ggACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATg < 1:433335/139‑1 (MQ=255) gACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAATGGa < 1:508484/77‑1 (MQ=255) gACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAATGGa > 2:508484/1‑77 (MQ=255) cAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAATGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCgg < 1:527841/150‑1 (MQ=255) aaaGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCgg > 1:276225/2‑150 (MQ=255) gACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGgc > 2:7230/1‑150 (MQ=255) cTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCa > 1:424489/1‑150 (MQ=255) cTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCa > 1:483547/1‑150 (MQ=255) tGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACg < 2:1380354/111‑1 (MQ=255) tGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGCTATTCGCGTGGTGCAGGAGACTGCAACg > 1:1380354/1‑111 (MQ=255) tGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAACGCGGCCCCAGCtttt > 1:23374/1‑150 (MQ=255) gATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGtttttt > 1:405040/1‑150 (MQ=255) aTGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTc < 1:1419392/144‑1 (MQ=255) aTGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTc > 2:1419392/1‑144 (MQ=255) gCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCg < 1:1356121/128‑1 (MQ=255) gCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCg > 2:1356121/1‑128 (MQ=255) cTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGa < 2:1138179/150‑1 (MQ=255) aaCGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTa > 2:1069163/1‑150 (MQ=255) aCGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTac < 2:483547/150‑1 (MQ=255) cGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTaca < 1:1374706/150‑1 (MQ=255) cGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTaca < 2:276225/150‑1 (MQ=255) gTATGCGCGTAACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACtt < 2:975226/150‑1 (MQ=255) tATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTg < 2:23374/150‑1 (MQ=255) aTGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGc < 2:996668/150‑1 (MQ=255) cgcgTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTca < 1:1069163/150‑1 (MQ=255) gACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAg > 2:1182082/1‑78 (MQ=255) gACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAg < 1:1182082/78‑1 (MQ=255) ccGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTCACAGCAAt > 1:584800/1‑150 (MQ=255) aaaGGCCGAAGGGATCGACATTCCGTTTCTGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACATTTGCGCACAGCAATTTCCAg < 2:1254776/149‑1 (MQ=255) aaGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGCCCCTGATTaca < 2:707703/128‑1 (MQ=255) aaGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTaca > 1:707703/1‑128 (MQ=255) gAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTCACAGCAATTTCCAGCCATTTc > 1:258124/1‑150 (MQ=255) gggATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTCACAGCAATTTCCAGCCATTTCTgg < 1:7230/150‑1 (MQ=255) | AAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATT‑ACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTCGCGTGGTGCAGGAGACTGCAACGCAGTAATGCCGCGTTCAAGCCGGGGCGCAAAGCGGCCCCAGCTTTTTCCGACCCTGATTACACTTGCTCACAGCAATTTCCAGCCATTTCTGG > NC_002947/1542906‑1543198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |