Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,592,029	T→G	22.9%	intergenic (‑94/‑109)	PP_1395 ← / → PP_1396	AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,592,029	0	T	G	22.9%	119.8 / 37.5	70	intergenic (‑94/‑109)	PP_1395/PP_1396	AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base G (10/6);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

AGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATC  >  NC_002947/1591883‑1592176                                                                                                                                                   |                                                                                                                                                    aGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCa                                                                                                                                                  >  2:211017/1‑150 (MQ=255)    tCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCCGATCCTGCAGCGCGGCAGGa                                                                                                                                               <  2:636805/150‑1 (MQ=255)      cAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATc                                                                                                                                             >  2:338716/1‑150 (MQ=255)       gggtgcTGCAGTTCCAGGGTCGGTTCGGGGGATGTGGCGGTGGTCATGCTGGCTTGCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCa                                                                                                                                            <  2:421611/146‑1 (MQ=38)       aGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCa                                                                                                                                            <  2:1072952/150‑1 (MQ=255)         gcgcTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAgg                                                                                                                                          <  1:154333/150‑1 (MQ=255)         gcgcTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGACACGGCCGCGCTGCAGGATCAgg                                                                                                                                          <  2:127099/150‑1 (MQ=255)         cagcTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGCTGGCTTTCCTCTTCTCACCGACGCAGCATAGGTTTGTGCAAAGACGCGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCCGCTCTGCAGGTTCAgg                                                                                                                                          <  2:266683/148‑1 (MQ=255)                  ttCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACa                                                                                                                                 >  2:521390/1‑150 (MQ=255)                     cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGc                                                                                                                              <  2:147664/150‑1 (MQ=255)                     cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGc                                                                                                                              >  2:59333/1‑150 (MQ=255)                     cAGGGTCGGTTCGGGGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAgg                                                                                                                                          >  2:1293190/1‑138 (MQ=255)                     cAGGGTCGGTTCGGGGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAgg                                                                                                                                          <  1:1293190/138‑1 (MQ=255)                        ggTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGt                                                                                                                           >  1:1256922/1‑150 (MQ=255)                                  gtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTaa                                                                                                                 >  1:1183204/1‑150 (MQ=255)                                      aCGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgcg                                                                                                             <  1:521390/150‑1 (MQ=255)                                              gtggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCgg                                                                                                     <  2:1256922/150‑1 (MQ=255)                                                    atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGAc                                                                                                                                  >  1:867356/1‑115 (MQ=255)                                                    atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGAc                                                                                                                                  <  2:867356/115‑1 (MQ=255)                                                       atgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCa                                                                                                                                            >  1:507612/1‑102 (MQ=37)                                                       atgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCa                                                                                                                                            <  2:507612/102‑1 (MQ=37)                                                           cTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAAc                                                                                        >  1:1137532/1‑150 (MQ=255)                                                              tCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCtt                                                                                     <  1:662853/150‑1 (MQ=255)                                                                ctctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTgg                                                                                   <  2:289991/150‑1 (MQ=255)                                                                      tgACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCTGCGCTGCAGGATCAgg                                                                                                                                          >  2:153632/1‑89 (MQ=255)                                                                      tgACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCTGCGCTGCAGGATCAgg                                                                                                                                          <  1:153632/89‑1 (MQ=255)                                                                       gACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGGGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                            <  2:270047/150‑1 (MQ=255)                                                                              gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCggg                                                                                                    >  2:462414/1‑119 (MQ=255)                                                                              gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCggg                                                                                                    <  1:462414/119‑1 (MQ=255)                                                                              gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                     >  1:154136/1‑150 (MQ=255)                                                                                 gcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgt                                                                  <  1:338716/150‑1 (MQ=255)                                                                                   aTAGGTTTGGGCCAAGACGGGCGTTAGTCCCAAGCCGCATTTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                <  2:373578/150‑1 (MQ=255)                                                                                   aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                <  2:154136/150‑1 (MQ=255)                                                                                   aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                                    <  1:829732/146‑1 (MQ=255)                                                                                   aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                                    >  2:829732/1‑146 (MQ=255)                                                                                       gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                            <  2:480692/134‑1 (MQ=255)                                                                                       gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                            >  1:480692/1‑134 (MQ=255)                                                                                                      ggCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATtg                                             <  2:1137532/150‑1 (MQ=255)                                                                                                                      tGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                                 >  2:722039/1‑82 (MQ=255)                                                                                                                      tGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                                 <  1:722039/82‑1 (MQ=255)                                                                                                                         aTGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                          >  2:956931/1‑150 (MQ=255)                                                                                                                         aTGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                          >  1:1313417/1‑150 (MQ=255)                                                                                                                         aTGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                          >  1:307168/1‑150 (MQ=255)                                                                                                                          tgtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaac                         >  1:503705/1‑150 (MQ=255)                                                                                                                           gtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAAcc                        >  1:1014005/1‑150 (MQ=255)                                                                                                                                   ccTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccc                >  2:76449/1‑150 (MQ=255)                                                                                                                                    cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc                                                                 <  2:933728/100‑1 (MQ=255)                                                                                                                                    cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc                                                                 >  1:933728/1‑100 (MQ=255)                                                                                                                                    cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca               >  2:892500/1‑150 (MQ=255)                                                                                                                                    cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca               >  2:326235/1‑150 (MQ=255)                                                                                                                                    cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGTAAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca               >  2:711507/1‑150 (MQ=255)                                                                                                                                    cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca               >  2:1394673/1‑150 (MQ=255)                                                                                                                                    cTGATCCTGCAGCGCGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca               >  2:788060/1‑150 (MQ=255)                                                                                                                                     tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATTCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccac              >  1:824214/1‑150 (MQ=255)                                                                                                                                     tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                     <  2:1376525/95‑1 (MQ=255)                                                                                                                                     tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                     >  1:1376525/1‑95 (MQ=255)                                                                                                                                     tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGAtt                                              >  1:1109932/1‑118 (MQ=255)                                                                                                                                     tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGAtt                                              <  2:1109932/118‑1 (MQ=255)                                                                                                                                     tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCt                                                                             >  2:1396410/1‑87 (MQ=255)                                                                                                                                     tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCt                                                                             <  1:1396410/87‑1 (MQ=255)                                                                                                                                     tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccac              >  1:966018/1‑150 (MQ=255)                                                                                                                                      gATCCTGCAGCCCGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccacc             >  2:172060/1‑150 (MQ=255)                                                                                                                                       aTCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCt            >  1:415294/1‑150 (MQ=255)                                                                                                                                            gCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCtt       <  2:1313417/150‑1 (MQ=255)                                                                                                                                            gCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCtt       <  2:503705/150‑1 (MQ=255)                                                                                                                                              cgcgCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCAGTCTGCTTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTc     <  2:194407/150‑1 (MQ=255)                                                                                                                                               gcgcGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                <  2:1041508/90‑1 (MQ=255)                                                                                                                                               gcgcGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                >  1:1041508/1‑90 (MQ=255)                                                                                                                                                cgcTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGGGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCAt   >  1:179405/1‑150 (MQ=255)                                                                                                                                                 gcTGCAGGAGCAGGCAATCGACAGGCAGTGATGGCCGAACGGGCCGCGCGGGGGAGCGCCGAGCCTTGGCAGCCGGGCTTTTCCGGCCGCCGGCTTGAAGGGGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATc  <  2:204417/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATC  >  NC_002947/1591883‑1592176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑