Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,592,046	G→C	7.6%	intergenic (‑111/‑92)	PP_1395 ← / → PP_1396	AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,592,046	0	G	C	7.6%	205.2 / 7.4	66	intergenic (‑111/‑92)	PP_1395/PP_1396	AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base C (3/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

TTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCGGG  >  NC_002947/1591900‑1592191                                                                                                                                                   |                                                                                                                                                  ttCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACa                                                                                                                                                >  2:521390/1‑150 (MQ=255)    cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGc                                                                                                                                             <  2:147664/150‑1 (MQ=255)    cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGc                                                                                                                                             >  2:59333/1‑150 (MQ=255)       ggTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGt                                                                                                                                          >  1:1256922/1‑150 (MQ=255)                 gtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTaa                                                                                                                                >  1:1183204/1‑150 (MQ=255)                     aCGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgcg                                                                                                                            <  1:521390/150‑1 (MQ=255)                             gtggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCgg                                                                                                                    <  2:1256922/150‑1 (MQ=255)                                   atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGAc                                                                                                                                                 <  2:867356/115‑1 (MQ=255)                                   atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGAc                                                                                                                                                 >  1:867356/1‑115 (MQ=255)                                          cTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAAc                                                                                                       >  1:1137532/1‑150 (MQ=255)                                             tCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCtt                                                                                                    <  1:662853/150‑1 (MQ=255)                                               ctctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTgg                                                                                                  <  2:289991/150‑1 (MQ=255)                                                      gACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGGGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                                           <  2:270047/150‑1 (MQ=255)                                                             gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCggg                                                                                                                   >  2:462414/1‑119 (MQ=255)                                                             gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCggg                                                                                                                   <  1:462414/119‑1 (MQ=255)                                                             gcagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                                    >  1:154136/1‑150 (MQ=255)                                                                gcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgt                                                                                 <  1:338716/150‑1 (MQ=255)                                                                  aTAGGTTTGGGCCAAGACGGGCGTTAGTCCCAAGCCGCATTTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                               <  2:373578/150‑1 (MQ=255)                                                                  aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                               <  2:154136/150‑1 (MQ=255)                                                                  aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                                                   >  2:829732/1‑146 (MQ=255)                                                                  aTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                                                   <  1:829732/146‑1 (MQ=255)                                                                      gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                                           <  2:480692/134‑1 (MQ=255)                                                                      gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTg                                                                                           >  1:480692/1‑134 (MQ=255)                                                                                     ggCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATtg                                                            <  2:1137532/150‑1 (MQ=255)                                                                                                     tGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                                                >  2:722039/1‑82 (MQ=255)                                                                                                     tGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                                                <  1:722039/82‑1 (MQ=255)                                                                                                        aTGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                                         >  2:956931/1‑150 (MQ=255)                                                                                                        aTGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                                         >  1:1313417/1‑150 (MQ=255)                                                                                                        aTGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaa                                         >  1:307168/1‑150 (MQ=255)                                                                                                         tgtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaacaac                                        >  1:503705/1‑150 (MQ=255)                                                                                                          gtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAAcc                                       >  1:1014005/1‑150 (MQ=255)                                                                                                                  ccTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccc                               >  2:76449/1‑150 (MQ=255)                                                                                                                   cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc                                                                                >  1:933728/1‑100 (MQ=255)                                                                                                                   cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc                                                                                <  2:933728/100‑1 (MQ=255)                                                                                                                   cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca                              >  2:326235/1‑150 (MQ=255)                                                                                                                   cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca                              >  2:892500/1‑150 (MQ=255)                                                                                                                   cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGTAAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca                              >  2:711507/1‑150 (MQ=255)                                                                                                                   cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca                              >  2:1394673/1‑150 (MQ=255)                                                                                                                   cTGATCCTGCAGCGCGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccca                              >  2:788060/1‑150 (MQ=255)                                                                                                                    tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATTCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccac                             >  1:824214/1‑150 (MQ=255)                                                                                                                    tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                                    >  1:1376525/1‑95 (MQ=255)                                                                                                                    tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                                                    <  2:1376525/95‑1 (MQ=255)                                                                                                                    tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGAtt                                                             >  1:1109932/1‑118 (MQ=255)                                                                                                                    tGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGAtt                                                             <  2:1109932/118‑1 (MQ=255)                                                                                                                    tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCt                                                                                            >  2:1396410/1‑87 (MQ=255)                                                                                                                    tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCt                                                                                            <  1:1396410/87‑1 (MQ=255)                                                                                                                    tGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccac                             >  1:966018/1‑150 (MQ=255)                                                                                                                     gATCCTGCAGCCCGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccacc                            >  2:172060/1‑150 (MQ=255)                                                                                                                      aTCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCt                           >  1:415294/1‑150 (MQ=255)                                                                                                                           gCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCtt                      <  2:503705/150‑1 (MQ=255)                                                                                                                           gCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCtt                      <  2:1313417/150‑1 (MQ=255)                                                                                                                             cgcgCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCAGTCTGCTTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTc                    <  2:194407/150‑1 (MQ=255)                                                                                                                              gcgcGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                               >  1:1041508/1‑90 (MQ=255)                                                                                                                              gcgcGGCAGGATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtcc                                                                               <  2:1041508/90‑1 (MQ=255)                                                                                                                               cgcTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGGGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCAt                  >  1:179405/1‑150 (MQ=255)                                                                                                                                gcTGCAGGAGCAGGCAATCGACAGGCAGTGATGGCCGAACGGGCCGCGCGGGGGAGCGCCGAGCCTTGGCAGCCGGGCTTTTCCGGCCGCCGGCTTGAAGGGGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATc                 <  2:204417/150‑1 (MQ=255)                                                                                                                                       gATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCca          <  1:892500/150‑1 (MQ=255)                                                                                                                                       gATCAGGCAATCCACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCca          <  1:788060/150‑1 (MQ=255)                                                                                                                                         tCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCCAGGATAGTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCcagc        >  2:403646/1‑150 (MQ=255)                                                                                                                                         tCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCcagc        >  2:592088/1‑150 (MQ=255)                                                                                                                                            ggCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCagcagc     >  2:884182/1‑150 (MQ=255)                                                                                                                                              cAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCgg   <  1:884182/150‑1 (MQ=255)                                                                                                                                              cAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCgg   <  1:326235/150‑1 (MQ=255)                                                                                                                                              cAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCgg   <  1:59333/150‑1 (MQ=255)                                                                                                                                               aaTCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAAGCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCggg  <  2:824214/150‑1 (MQ=255)                                                                                                                                               aaTCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCggg  >  1:1207749/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGCTTTCATCCGTGCCAGCAGCGGG  >  NC_002947/1591900‑1592191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑