| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,594,115 | G→A | 10.6% | intergenic (‑271/+30) | PP_1399 ← / ← kgtP | hypothetical protein/alpha‑ketoglutarate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,594,115 | 0 | G | A | 10.6% | 121.4 / 4.8 | 47 | intergenic (‑271/+30) | PP_1399/kgtP | hypothetical protein/alpha‑ketoglutarate permease |
| Reads supporting (aligned to +/- strand): ref base G (20/22); new base A (2/3); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGG > NC_002947/1593966‑1594257 | gcgTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATggg > 2:66082/1‑150 (MQ=255) tGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCttt < 2:789418/150‑1 (MQ=255) aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGCAc < 1:1425132/150‑1 (MQ=255) ggCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACt > 1:95754/1‑150 (MQ=255) ggCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGC‑ATGGAGCGCCCCttttctattc < 2:593237/150‑7 (MQ=38) cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATgggg < 2:1210930/131‑1 (MQ=255) cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATgggg > 1:1210930/1‑131 (MQ=255) gCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTgcca < 2:95754/150‑1 (MQ=255) aaGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATCAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTgccagg > 1:1229015/1‑150 (MQ=255) aGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCTTTTTAGTACTgccaggc < 1:608742/150‑1 (MQ=255) gAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCa > 2:226878/1‑150 (MQ=255) cGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCGA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg < 1:66082/150‑1 (MQ=255) ttCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGt > 2:541370/1‑150 (MQ=255) gggCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCcgc < 1:1208211/150‑1 (MQ=255) gcgcCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGa < 1:541370/150‑1 (MQ=255) cAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgc > 2:335401/1‑150 (MQ=255) ttCTGCCAGCCATTGCAGCTTGAAAAGGTGGAATGAATAGGGCGTTAAGAGGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCtt > 2:1378832/1‑150 (MQ=255) tGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgt < 1:226878/150‑1 (MQ=255) ccaTTGCAGCTTGATAAGGGGGAATTAATAGGGCGGTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCttt > 1:1177071/1‑150 (MQ=255) caTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTc < 2:269604/150‑1 (MQ=255) ttGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGTCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCAt < 2:91472/150‑1 (MQ=255) cTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTcgc > 1:182952/1‑150 (MQ=255) gATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTa > 1:822017/1‑150 (MQ=255) aGGTGGAATTAATAGGGCGGTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGTTCCA‑GGCAATGGGGAGCCCCTATTAATACTGCCAGGCCAGCGTTACTCGGTGGTAATCCGCGAGTGCTGCCTGGTGTATTTGATGGTCGCGTAGAcg > 2:540649/1‑149 (MQ=255) tGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACcagc > 1:1234786/1‑150 (MQ=255) ggAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACcagca < 1:409311/150‑1 (MQ=255) ggAATTAATACGACGTCAAGAAGGAAAAGTGTGAATATAAAATGGGCCCGCCA‑TACAATGGGGGCCCCCTTTTAGTACTGCCAGGCCAGCGTTACGCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCACGGTCGCGTAAACcagca < 2:392512/150‑1 (MQ=255) ttAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTc < 2:1060082/123‑1 (MQ=255) ttAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTc > 1:1060082/1‑123 (MQ=255) aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGccc < 2:813121/61‑1 (MQ=255) aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGccc > 1:813121/1‑61 (MQ=255) aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCACGGAGGGTCGCGTAAACCAGCAGCGAGCa < 1:37321/150‑1 (MQ=255) ttAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATAc > 1:1131120/1‑150 (MQ=255) agaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTcgc < 2:1210407/121‑1 (MQ=255) agaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTcgc > 1:1210407/1‑121 (MQ=255) agaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGCCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGCGTCCTTCATGGCCGCGTGCACCGGCAGCGTGGAGGCGCTACAgg > 2:7058/1‑150 (MQ=255) gcagGAAAAGTCTGAAGAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACGCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCATCGAGCAGGCGATACAggg > 2:1096358/3‑149 (MQ=255) aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCg < 2:1229015/150‑1 (MQ=255) agGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATggg > 1:811381/1‑41 (MQ=255) agGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATggg < 2:811381/41‑1 (MQ=255) ggAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGt < 1:1378832/150‑1 (MQ=255) tCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc < 1:488271/150‑1 (MQ=255) tCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc < 2:822017/150‑1 (MQ=255) tCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc > 2:1205499/1‑150 (MQ=255) tGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCa < 1:1205499/150‑1 (MQ=255) tGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGGGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCa > 2:754092/1‑150 (MQ=255) aaaGGGGCGCCCCATTGC‑ATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAgg > 1:950916/1‑120 (MQ=38) aaaGGGGCGCCCCATTGC‑ATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAgg < 2:950916/120‑1 (MQ=38) aaaGGGGCGCCCCA‑TTGCATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGt > 1:450539/1‑133 (MQ=39) aaaGGGGCGCCCCA‑TTGCATGGAGCGCACCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGTAGCGAGCAGGCGATACAGGCGGt < 2:450539/133‑1 (MQ=255) tGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCg < 2:1131120/150‑1 (MQ=255) gCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCgg > 1:1164105/1‑150 (MQ=255) | GCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCA‑TGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGG > NC_002947/1593966‑1594257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |