Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,683,290 C→G 8.2% intergenic (+57/‑174) recJ → / → PP_1478 ssDNA‑specific exonuclease RecJ/xenobiotic reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,683,2900CG 8.2% 143.8 / 5.1 49intergenic (+57/‑174)recJ/PP_1478ssDNA‑specific exonuclease RecJ/xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base G (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

TTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGG  >  NC_002947/1683153‑1683428
                                                                                                                                         |                                                                                                                                          
ttGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                >  2:1339613/1‑150 (MQ=255)
 tGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATGTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                               <  1:523204/150‑1 (MQ=255)
       aCAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTca                                                                                                                         <  1:841127/150‑1 (MQ=255)
                 tgtgAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGc                                                                                                               <  2:1075844/150‑1 (MQ=255)
                 tgtgAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGc                                                                                                               <  1:35515/150‑1 (MQ=255)
                     aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              <  1:703823/131‑1 (MQ=255)
                     aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              >  2:703823/1‑131 (MQ=255)
                     aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATGGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                           >  1:1101678/1‑150 (MQ=255)
                         aGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCttt                                                                                                       <  1:787534/150‑1 (MQ=255)
                                     aTGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGCCGCGCGGGCGGCGCTCGAtttgtgc                                                                                                                       <  1:740804/122‑7 (MQ=38)
                                     aTGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGCCGCGCGGGCGGCGCTCGAtttgtgc                                                                                                                       >  2:740804/1‑116 (MQ=38)
                                       gagaGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAtctc                                                                                                                          >  2:902629/1‑117 (MQ=255)
                                       gagaGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAtctc                                                                                                                          <  1:902629/117‑1 (MQ=255)
                                                cAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCgccgcc                                                                                                                                            >  2:629701/1‑90 (MQ=255)
                                                cAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCgccgcc                                                                                                                                            <  1:629701/90‑1 (MQ=255)
                                                 aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCcaca                                                                               >  2:258784/1‑150 (MQ=255)
                                                 aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCcaca                                                                               >  1:580099/1‑150 (MQ=255)
                                                   ttGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGa                                                                             >  1:650799/1‑150 (MQ=255)
                                                     gatgatTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc                                                                           >  1:819362/1‑150 (MQ=255)
                                                             cgcATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTcccccc                                                                   <  1:462716/150‑1 (MQ=255)
                                                                atatGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGACCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGGGCACCCCTCGCCATCCCACAGAACCTCCCCccacc                                                                >  2:1319370/1‑150 (MQ=255)
                                                                        ccGCGCTGATTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGt                                                        >  2:20219/1‑150 (MQ=255)
                                                                          gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCt                                                      <  2:975523/150‑1 (MQ=255)
                                                                            gcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAg                                                    >  2:99543/1‑150 (MQ=255)
                                                                                          cAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAcc                                      <  1:20219/150‑1 (MQ=255)
                                                                                           aGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTgg                                                         <  2:593619/130‑1 (MQ=255)
                                                                                           aGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTgg                                                         >  1:593619/1‑130 (MQ=255)
                                                                                             gTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGc                                   >  1:511480/1‑150 (MQ=255)
                                                                                                 ctgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCg                               >  2:1342176/1‑150 (MQ=255)
                                                                                                  tgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCgg                              >  2:571087/1‑150 (MQ=255)
                                                                                                     ctTGGATCTTGCGCTGGGGCACAAATCGATCGACGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAAGCTGGTGTAGTCTCATTAATGACCGGCACCGGGcc                           >  1:19012/1‑150 (MQ=255)
                                                                                                     ctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGcc                           >  1:432772/1‑150 (MQ=255)
                                                                                                         gATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAggg                       >  2:795298/1‑150 (MQ=255)
                                                                                                           tCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                     <  2:511480/150‑1 (MQ=255)
                                                                                                             ttGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAGCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAtgt                   >  2:291/1‑150 (MQ=255)
                                                                                                               gcgcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGc                 >  2:1424548/1‑150 (MQ=255)
                                                                                                                 gcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca                                                                  >  1:951487/1‑99 (MQ=255)
                                                                                                                 gcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca                                                                  <  2:951487/99‑1 (MQ=255)
                                                                                                                     gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCTCTCGCCATCCCCCAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGCTGTGCACTTGa           <  2:668809/150‑1 (MQ=255)
                                                                                                                     gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGTACTTGa           <  1:143190/150‑1 (MQ=255)
                                                                                                                     gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa           <  1:795298/150‑1 (MQ=255)
                                                                                                                      ggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAg          <  1:258784/150‑1 (MQ=255)
                                                                                                                       gCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgt         >  1:695694/1‑150 (MQ=255)
                                                                                                                          gaaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTc      >  1:122775/2‑150 (MQ=255)
                                                                                                                          caAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTc      <  2:650799/150‑1 (MQ=255)
                                                                                                                           aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc                                                                           >  2:1191550/1‑80 (MQ=255)
                                                                                                                           aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc                                                                           <  1:1191550/80‑1 (MQ=255)
                                                                                                                           aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTcc     >  1:243833/1‑150 (MQ=255)
                                                                                                                            aaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCg    <  1:1342176/150‑1 (MQ=255)
                                                                                                                             aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCgg   <  1:1424548/150‑1 (MQ=255)
                                                                                                                              tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCggg  >  2:192301/1‑150 (MQ=255)
                                                                                                                                    gccgccCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa           >  2:916614/1‑135 (MQ=255)
                                                                                                                                    gccgccCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa           <  1:916614/135‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGG  >  NC_002947/1683153‑1683428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑