| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,683,293 | C→G | 7.8% | intergenic (+60/‑171) | recJ → / → PP_1478 | ssDNA‑specific exonuclease RecJ/xenobiotic reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,683,293 | 0 | C | G | 7.8% | 154.0 / 5.0 | 51 | intergenic (+60/‑171) | recJ/PP_1478 | ssDNA‑specific exonuclease RecJ/xenobiotic reductase |
| Reads supporting (aligned to +/- strand): ref base C (26/21); new base G (2/2); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01 | |||||||||||
TTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCACCATTG > NC_002947/1683153‑1683442 | ttGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTc > 2:1339613/1‑150 (MQ=255) tGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATGTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCg < 1:523204/150‑1 (MQ=255) aCAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTca < 1:841127/150‑1 (MQ=255) tgtgAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGc < 1:35515/150‑1 (MQ=255) tgtgAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGc < 2:1075844/150‑1 (MQ=255) aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCTCGa < 1:703823/131‑1 (MQ=255) aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCTCGa > 2:703823/1‑131 (MQ=255) aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATGGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc > 1:1101678/1‑150 (MQ=255) aGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCttt < 1:787534/150‑1 (MQ=255) aTGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGCCGCGCGGGCGGCGCTCGAtttgtgc > 2:740804/1‑116 (MQ=38) aTGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGCCGCGCGGGCGGCGCTCGAtttgtgc < 1:740804/122‑7 (MQ=38) gagaGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAtctc < 1:902629/117‑1 (MQ=255) gagaGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAtctc > 2:902629/1‑117 (MQ=255) aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCcaca > 2:258784/1‑150 (MQ=255) aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCcaca > 1:580099/1‑150 (MQ=255) ttGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGa > 1:650799/1‑150 (MQ=255) gatgatTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc > 1:819362/1‑150 (MQ=255) cgcATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTcccccc < 1:462716/150‑1 (MQ=255) atatGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGACCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGGGCACCCCTCGCCATCCCACAGAACCTCCCCccacc > 2:1319370/1‑150 (MQ=255) ccGCGCTGATTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGt > 2:20219/1‑150 (MQ=255) gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCt < 2:975523/150‑1 (MQ=255) gcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAg > 2:99543/1‑150 (MQ=255) cAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAcc < 1:20219/150‑1 (MQ=255) aGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTgg > 1:593619/1‑130 (MQ=255) aGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTgg < 2:593619/130‑1 (MQ=255) gTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGc > 1:511480/1‑150 (MQ=255) ctgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCg > 2:1342176/1‑150 (MQ=255) tgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCgg > 2:571087/1‑150 (MQ=255) ctTGGATCTTGCGCTGGGGCACAAATCGATCGACGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAAGCTGGTGTAGTCTCATTAATGACCGGCACCGGGcc > 1:19012/1‑150 (MQ=255) ctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGcc > 1:432772/1‑150 (MQ=255) gATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAggg > 2:795298/1‑150 (MQ=255) tCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt < 2:511480/150‑1 (MQ=255) ttGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGAGCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAtgt > 2:291/1‑150 (MQ=255) gcgcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGc > 2:1424548/1‑150 (MQ=255) gcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca > 1:951487/1‑99 (MQ=255) gcTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca < 2:951487/99‑1 (MQ=255) gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCTCTCGCCATCCCCCAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGCTGTGCACTTGa < 2:668809/150‑1 (MQ=255) gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGTACTTGa < 1:143190/150‑1 (MQ=255) gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa < 1:795298/150‑1 (MQ=255) ggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAg < 1:258784/150‑1 (MQ=255) gCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgt > 1:695694/1‑150 (MQ=255) gaaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTc > 1:122775/2‑150 (MQ=255) caAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTc < 2:650799/150‑1 (MQ=255) aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc > 2:1191550/1‑80 (MQ=255) aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAc < 1:1191550/80‑1 (MQ=255) aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTcc > 1:243833/1‑150 (MQ=255) aaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCg < 1:1342176/150‑1 (MQ=255) aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCgg < 1:1424548/150‑1 (MQ=255) tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCggg > 2:192301/1‑150 (MQ=255) gccgccCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa < 1:916614/135‑1 (MQ=255) gccgccCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa > 2:916614/1‑135 (MQ=255) cgcgcgGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCACCAt > 2:796163/1‑150 (MQ=255) cgcgGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCACCATTg < 1:1339613/150‑1 (MQ=255) | TTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCACCATTG > NC_002947/1683153‑1683442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |