Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,713,557 A→C 5.8% A153A (GCA→GCC)  tsaB → endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,713,5570AC 5.8% 227.2 / 4.5 79A153A (GCA→GCC) tsaBendopeptidase
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base C (2/3);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TTGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCG  >  NC_002947/1713408‑1713702
                                                                                                                                                     |                                                                                                                                                 
ttGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCggca                                                                                                                                                   <  1:216260/150‑1 (MQ=255)
        gCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCaa                                                                                                                                           <  2:1063708/150‑1 (MQ=255)
        gCATGGCGTGCAGCAGGTGGCCGCTGCCATCAATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCTGCCGgcagcgc                                                                                                                                           <  2:1158953/150‑3 (MQ=255)
            ggCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGc                                                                                                                                       >  2:1410087/1‑150 (MQ=255)
                    gcagGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCATGAAGCCGTGTTGCCGCCCGACCAGGTGGCGCGGCCGGCAGGGAGCAATGGCGAATGGtt                                                                                                                               >  2:590125/1‑150 (MQ=255)
                     cagGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTc                                                                                                                              <  2:775474/150‑1 (MQ=255)
                      agGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCg                                                                                                                             <  1:662046/150‑1 (MQ=255)
                      agGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCg                                                                                                                             >  2:545416/1‑150 (MQ=255)
                      agGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGCTCGGCCGTGAAGGCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCg                                                                                                                             <  2:984255/150‑1 (MQ=255)
                            ccGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCg                                                                                                                       <  1:476185/150‑1 (MQ=255)
                                  ccATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCg                                                                                                                 >  2:1196366/1‑150 (MQ=255)
                                     tCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCTGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGt                                                                                                              <  1:3629/150‑1 (MQ=255)
                                       gATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                            >  1:598470/1‑150 (MQ=255)
                                       gATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAACAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                            >  2:676544/1‑150 (MQ=255)
                                        aTGCGCGCATGGATGAGGGTTACTGGGCCCGCTACCAGGCCCCAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGAGGCGCTGCGGGCAGGCGCCAAGGGCGAGAGGTCCGGAGCCGCTACgggggggg                                                                                                           >  2:124979/1‑150 (MQ=255)
                                           cgcgcATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCt                                                                                                        <  1:1410087/150‑1 (MQ=255)
                                                   gATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGAt                                                                                                >  2:27597/1‑150 (MQ=255)
                                                    aTGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAACAATGGCGATTTGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATc                                                                                               <  1:27597/150‑1 (MQ=255)
                                                     tGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCg                                                                                              >  2:1332557/1‑150 (MQ=255)
                                                         gTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTg                                                                                          >  2:244995/1‑150 (MQ=255)
                                                           ttACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGc                                                                                        <  1:573176/150‑1 (MQ=255)
                                                             aCTGGGGCTGCTACCAGGCCACAGCGGGCGCAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCgg                                                                                      >  2:182663/1‑150 (MQ=255)
                                                              cTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGt                                                                                     >  2:159575/1‑150 (MQ=255)
                                                                 gggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                            >  1:850395/1‑124 (MQ=255)
                                                                 gggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                            <  2:850395/124‑1 (MQ=255)
                                                                 gggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACa                                                                                  >  2:508236/1‑150 (MQ=255)
                                                                  ggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAg                                                                                 <  2:625702/150‑1 (MQ=255)
                                                                  ggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTACCGGGAGTGGGTGGGGCTATGCCGATCGCCTGGCGGTACAg                                                                                 >  2:691169/1‑150 (MQ=255)
                                                                      gctACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAg                                                                             >  2:566910/1‑150 (MQ=255)
                                                                        tACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcg                                                                           <  1:159575/150‑1 (MQ=255)
                                                                         aCCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACTGGGCGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcgg                                                                          >  1:785015/1‑150 (MQ=255)
                                                                          ccAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcggc                                                                         <  1:606893/150‑1 (MQ=255)
                                                                           cAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGGCGGCAGGCAGCAATGGCGAGCGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcggcg                                                                        >  2:917334/1‑150 (MQ=255)
                                                                           cAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcggcg                                                                        >  1:59889/1‑150 (MQ=255)
                                                                           cAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcggcg                                                                        >  2:1413006/1‑150 (MQ=255)
                                                                            agtccACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTAGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCTGCGa                                                                       >  2:353638/4‑150 (MQ=255)
                                                                            aGGCCACAGCGGGCGAAATGCGCCTGGTCGGTCGCGAAGCCGTGTTGCGGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGa                                                                       <  2:785015/150‑1 (MQ=255)
                                                                            aGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCATGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGa                                                                       <  1:590125/150‑1 (MQ=255)
                                                                             ggCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTggg                                                                                                           <  1:742846/113‑1 (MQ=255)
                                                                             ggCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTggg                                                                                                           >  2:742846/1‑113 (MQ=255)
                                                                                gcgatcGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCaa                                                                   >  1:1119264/6‑150 (MQ=255)
                                                                                cacaGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCaa                                                                   >  2:7169/1‑150 (MQ=255)
                                                                                 acaGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAAc                                                                  >  2:1102151/1‑150 (MQ=255)
                                                                                 acaGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAAc                                                                  <  1:508236/150‑1 (MQ=255)
                                                                                   aGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAAccc                                                                >  2:875017/1‑150 (MQ=255)
                                                                                       ggCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGATCGGTGCCGGTACCGGGTGGGGCTCTGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCt                                                            >  1:310488/1‑150 (MQ=255)
                                                                                         cGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGTGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGAGCGCCTAGCGGTACAGGTAGCGGCGAGCAACCCGGCTGc                                                          <  2:272856/150‑1 (MQ=255)
                                                                                          gAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGACCAGGTCGAGAAGCCGGCAGGCAGGAATTGCGAGGGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGcc                                                         >  1:388689/1‑150 (MQ=255)
                                                                                            aaTGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCtt                                                       <  1:244995/150‑1 (MQ=255)
                                                                                              tGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGc                                                     <  1:1196366/150‑1 (MQ=255)
                                                                                               gcgcCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGcc                                                    <  1:545416/150‑1 (MQ=255)
                                                                                               gcgcCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGCGCTATGCCGATCGCCTGTCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGcc                                                    <  2:1119264/150‑1 (MQ=255)
                                                                                                        cGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGctct                                           <  1:7169/150‑1 (MQ=255)
                                                                                                         ggCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTg                                          >  2:325649/1‑150 (MQ=255)
                                                                                                          gCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTgg                                         <  2:388689/150‑1 (MQ=255)
                                                                                                             gTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACa                                      >  1:265316/1‑150 (MQ=255)
                                                                                                                  gCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATtctc                                 >  1:997358/1‑150 (MQ=255)
                                                                                                                  gCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGAAAACCGGCTGCCTTGCCCAATGCTCTGGACATtctc                                 <  1:1102151/150‑1 (MQ=255)
                                                                                                                     gtgtTGCCGCCGGAGCAGGTGGCGCTGCCCGCAGGTAGCAATGGCGAGTGGTTCGGTGCCGGGACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGc                              <  2:310488/150‑1 (MQ=255)
                                                                                                                        ttGCCGCCCGAGCAGGTGGCGCTGCCGTCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTg                           <  1:314896/150‑1 (MQ=255)
                                                                                                                        ttGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcgg                                                                          >  2:309968/1‑103 (MQ=255)
                                                                                                                        ttGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcgg                                                                          <  1:309968/103‑1 (MQ=255)
                                                                                                                         tGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTAGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCCGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTgg                          <  1:1155720/150‑1 (MQ=255)
                                                                                                                                 gAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCttt                  <  1:182663/150‑1 (MQ=255)
                                                                                                                                     aGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATg                                                                                                     <  2:858486/63‑1 (MQ=255)
                                                                                                                                     aGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATg                                                                                                     >  1:858486/1‑63 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTgg            <  1:875017/150‑1 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTgg            >  1:793176/1‑150 (MQ=255)
                                                                                                                                         ggCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCtt                                                       <  2:613757/105‑1 (MQ=255)
                                                                                                                                         ggCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCtt                                                       >  1:613757/1‑105 (MQ=255)
                                                                                                                                         attgcTGCCTGCCGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGTGGTACAGGTAGCGGCGa                                                                       >  1:563039/4‑89 (MQ=255)
                                                                                                                                         attgcTGCCTGCCGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGTGGTACAGGTAGCGGCGa                                                                       <  2:563039/86‑1 (MQ=255)
                                                                                                                                          ttgcTGCCTGCCGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTg                           >  2:1315089/3‑132 (MQ=255)
                                                                                                                                          ttgcTGCCTGCCGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTg                           <  1:1315089/130‑1 (MQ=255)
                                                                                                                                          gcgcTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGcc         <  1:892590/150‑1 (MQ=255)
                                                                                                                                           cgcTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGTGccc        >  1:8676/1‑150 (MQ=255)
                                                                                                                                            gcTGCCGGCAGGCAGCAATGGCGAGTGGTACGGTGCCGGTACCGGGTGGGGCTAGGCCGAACGCCTGGCTGTAAAGGTAGAAACTGACCACCAGGCGGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCCACTTTGCCTGGGCCCg       >  1:678543/1‑150 (MQ=255)
                                                                                                                                              tGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTg     >  1:761700/1‑150 (MQ=255)
                                                                                                                                              tGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTg     <  1:485321/150‑1 (MQ=255)
                                                                                                                                                 cGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCg  >  1:143747/1‑150 (MQ=255)
                                                                                                                                                   gcaggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCg                                                               <  1:302695/87‑1 (MQ=255)
                                                                                                                                                   gcaggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCg                                                               >  2:302695/1‑87 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCG  >  NC_002947/1713408‑1713702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑