| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,718,111 | G→C | 14.5% | L256F (TTG→TTC) | rsmJ → | ribosomal RNA small subunit methyltransferase J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,718,111 | 0 | G | C | 14.5% | 129.2 / 15.1 | 49 | L256F (TTG→TTC) | rsmJ | ribosomal RNA small subunit methyltransferase J |
| Reads supporting (aligned to +/- strand): ref base G (20/21); new base C (3/4); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01 | |||||||||||
GCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717965‑1718260 | gcgcTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAg < 2:913792/150‑1 (MQ=255) gctgctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGc > 2:543220/1‑150 (MQ=255) ctgctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGcc < 1:268183/150‑1 (MQ=255) tgctCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCt < 1:873542/150‑1 (MQ=255) tCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGAc > 1:650555/1‑150 (MQ=255) tCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGAc > 2:1244355/1‑150 (MQ=255) aaGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttcttcg < 1:543220/150‑7 (MQ=255) aaGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttcttcg < 2:1294409/150‑7 (MQ=255) ccGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGccc < 1:680024/150‑1 (MQ=255) tctGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTAt < 2:650555/150‑1 (MQ=255) tctGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttcttcg < 2:1186743/143‑7 (MQ=255) tctGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttcttcg > 1:1186743/1‑137 (MQ=255) gcTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGcc > 2:868969/1‑150 (MQ=255) tGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGcccc > 2:509859/1‑139 (MQ=255) tGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGcccc < 1:509859/139‑1 (MQ=255) agccaCCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGc > 2:622786/1‑150 (MQ=255) gggTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTg > 1:1089099/1‑150 (MQ=255) tAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAg > 2:1235427/1‑150 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTg < 1:535649/106‑1 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTg > 2:535649/1‑106 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTgg > 1:543599/1‑117 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTgg < 2:543599/117‑1 (MQ=255) ccACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGcc > 1:385182/1‑150 (MQ=255) cACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCg > 1:67019/1‑150 (MQ=255) cGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTg > 1:1365315/1‑103 (MQ=255) cGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTg < 2:1365315/103‑1 (MQ=255) gCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCAt > 1:415163/1‑150 (MQ=255) cGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttct > 2:224502/1‑72 (MQ=255) cGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttct < 1:224502/75‑4 (MQ=255) gCCGAGCCATAGCCGTGAGGGTAAGTCGAGCCGGTGTGACATCTACCCGAAGAAGGCGTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGAGTG‑TAGGAGCGGATATAGCGGCGAACACCGACAACGTCGGTGCCATacacgg > 2:101569/1‑148 (MQ=255) tAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGtctc > 1:698317/1‑150 (MQ=255) tAGCCTTGAGGGTAAGTCGAGCAGGTTGGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGtctc > 2:1194923/1‑150 (MQ=255) aGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGCCGTCTCCTGCGCGTTAAACCTGCTc < 1:1235427/150‑1 (MQ=255) cGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTa < 1:622786/150‑1 (MQ=255) gTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACa < 1:868969/150‑1 (MQ=255) gTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCACGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGt < 2:1273207/134‑1 (MQ=255) gTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCACGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGt > 1:1273207/1‑134 (MQ=255) aTGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAAt < 2:67019/150‑1 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGa > 2:321199/1‑149 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGa < 1:321199/149‑1 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGAc < 2:698317/150‑1 (MQ=255) aCATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGAc < 2:895375/150‑1 (MQ=255) aTCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCt < 2:385182/150‑1 (MQ=255) cccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGc > 1:707437/1‑131 (MQ=255) cccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGc < 2:707437/131‑1 (MQ=255) cccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTAt > 1:821402/1‑150 (MQ=255) aagaagGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCACCCCCGGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCt < 1:1244355/150‑1 (MQ=255) gaagGCCTTTACGTCCTGACCTGCCCCTATCTGGCAGGCTAGCCCGAATGAAACGACAGGTCATGGTTG‑GTACTCCTAAAGCGGCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGc < 1:101569/150‑1 (MQ=255) tGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTc < 1:1194923/150‑1 (MQ=255) gAAGGCCTGACCTGCCCCTATCTGGCCGGCCAGCACGATTG‑TAGGGGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCa > 1:275011/1‑150 (MQ=255) | GCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTG‑TAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717965‑1718260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |