| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,721,945 | T→A | 7.4% | L179Q (CTG→CAG) | PP_1517 → | RND family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,721,945 | 0 | T | A | 7.4% | 175.1 / 4.2 | 54 | L179Q (CTG→CAG) | PP_1517 | RND family transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/22); new base A (2/2); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
TTCAACGACGAGTTCGGCACCACCTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTCGAGACGCCCAGCGAGCGCT > NC_002947/1721806‑1722094 | ttcAACGACGAGTTCGGCACCACCTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTg > 2:1202652/1‑150 (MQ=255) aaCGACGAGTTCGGCACCACCTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAg > 2:1208729/1‑150 (MQ=255) acgaccAGTTCGGCAACACCTCAGGCCCTATCTATCGAAAAACTGGCGAATGCTTGGACTATGCTGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAgg < 2:1084564/150‑1 (MQ=255) ccaccTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGa < 1:362753/150‑1 (MQ=255) cTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGa < 1:1208729/150‑1 (MQ=255) cGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTATGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCt < 1:355433/150‑1 (MQ=255) ggCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTg > 1:1229173/1‑150 (MQ=255) gCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGt > 1:1158167/1‑150 (MQ=255) cAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTc > 1:463450/1‑150 (MQ=255) gcTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCt > 2:597925/1‑150 (MQ=255) gCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCAccc > 2:285695/1‑150 (MQ=255) gCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCg > 1:504600/1‑150 (MQ=255) tttGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTg > 1:1210406/1‑150 (MQ=255) gACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCg > 2:733756/1‑150 (MQ=255) gACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGCCGATCAGATCGACCttgc > 1:344030/1‑89 (MQ=255) gACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGCCGATCAGATCGACCttgc < 2:344030/92‑4 (MQ=255) aCTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGc > 2:1202199/1‑90 (MQ=255) aCTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGc < 1:1202199/90‑1 (MQ=255) cTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCt < 2:1158167/150‑1 (MQ=255) tATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTg > 1:706676/1‑150 (MQ=255) tGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGa > 2:99026/1‑150 (MQ=255) ggTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCa > 1:519032/1‑84 (MQ=255) ggTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCa < 2:519032/84‑1 (MQ=255) gCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGt < 2:463450/150‑1 (MQ=255) aGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGa > 2:461666/1‑150 (MQ=255) gACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAg < 2:504600/150‑1 (MQ=255) tACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGATGCCGTGCAGAAGGCg > 2:989479/1‑150 (MQ=255) gCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTg > 1:998766/1‑150 (MQ=255) ccGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGc > 1:439431/1‑150 (MQ=255) ccGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAaga < 2:1037164/150‑1 (MQ=255) tATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGCCGATCAGATCGACCtt > 1:1205655/1‑57 (MQ=37) tATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGCCGATCAGATCGACCtt < 2:1205655/58‑2 (MQ=37) tccagatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGa > 2:1292769/1‑150 (MQ=255) cagatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCAGTGCAGaa > 1:1211262/1‑131 (MQ=255) cagatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCAGTGCAGaa < 2:1211262/131‑1 (MQ=255) agatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAAcg < 2:998766/150‑1 (MQ=255) agatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAAcg > 2:816147/1‑150 (MQ=255) gatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGc > 1:1048214/1‑106 (MQ=255) gatccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGc < 2:1048214/106‑1 (MQ=255) tccagCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCgg < 2:1424852/150‑1 (MQ=255) agCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGc < 2:93378/127‑1 (MQ=255) agCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGc > 1:93378/1‑127 (MQ=255) agCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGa < 1:285695/150‑1 (MQ=255) agCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGa < 1:989479/150‑1 (MQ=255) gCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCAc > 1:1025124/1‑150 (MQ=255) gCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCAc < 1:99026/150‑1 (MQ=255) gTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGt > 1:320747/1‑150 (MQ=255) tCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGtt > 1:619826/1‑150 (MQ=255) tCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGtt < 1:1202652/150‑1 (MQ=255) tCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGtt > 2:1193617/1‑150 (MQ=255) gATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTC < 2:619826‑M1/150‑1 (MQ=255) aTGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTCG < 2:706676‑M1/150‑1 (MQ=255) tgtgGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTCGA > 2:1167322‑M1/1‑150 (MQ=255) gTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGAACAACCTCAAGCTGGTCATCCTTGGCGTTCCGCTTGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTCGAGACGCCCagc < 2:1229173/150‑1 (MQ=255) tGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCCAGAGAAGAAACAGACGAACACTGCCGCTTGCGCCAAGACGCCCAGCGCGCGCt > 2:373925/1‑150 (MQ=255) | TTCAACGACGAGTTCGGCACCACCTTCGGCAATATCTATGCGCTGACTGGCGAAGGCTTTGACTATGCGGTGCTCAAGGACTACGCCGACCGTATCCAGATCCAGCTGCAGCGGGTCAAGGATGTGGGCAAGGTCGATCTGATCGGCCTGCAGGACGAGAAAGTCTGGATCGAGCTGTCCAACCTCAAGCTGGCCACCCTCGGCGTGCCGCTGGAAGCCGTGCAGAAGGCGTTGCAAGAGCAGAACGCGGTGAGCACCGCCGGTTTCTTCGAGACGCCCAGCGAGCGCT > NC_002947/1721806‑1722094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |