| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,786,065 | T→A | 10.0% | intergenic (+39/‑147) | rpsB → / → tsf | 30S ribosomal protein S2/elongation factor Ts |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,786,065 | 0 | T | A | 10.0% | 118.0 / 5.0 | 40 | intergenic (+39/‑147) | rpsB/tsf | 30S ribosomal protein S2/elongation factor Ts |
| Reads supporting (aligned to +/- strand): ref base T (18/18); new base A (2/2); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
GAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGA > NC_002947/1785919‑1786210 | gAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGc < 2:1235528/150‑1 (MQ=255) tGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGccccc < 2:344038/150‑1 (MQ=255) cATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCtttt > 2:1128305/1‑150 (MQ=255) aTGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt < 1:217659/150‑1 (MQ=255) aCGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCct < 1:633327/150‑1 (MQ=255) cATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCa < 2:544329/150‑1 (MQ=255) ccGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCa < 1:1394635/150‑1 (MQ=255) acGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGcatca > 2:229601/1‑150 (MQ=255) aCCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTc > 2:794198/1‑118 (MQ=255) aCCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTc < 1:794198/118‑1 (MQ=255) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCCTCATGGGCCTGCtgaaatgaa < 1:474775/142‑1 (MQ=255) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTGTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaatgaa > 2:474775/1‑142 (MQ=255) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCttt < 2:713732/82‑1 (MQ=38) gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCttt > 1:713732/1‑82 (MQ=38) tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt > 2:1139981/1‑82 (MQ=255) tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt < 1:1139981/82‑1 (MQ=255) tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAt < 1:1128305/150‑1 (MQ=255) aagaagCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAttt > 2:63180/1‑150 (MQ=255) gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCtt < 2:1010306/83‑1 (MQ=255) gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCtt > 1:1010306/1‑83 (MQ=255) tgctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc > 1:222787/1‑150 (MQ=255) ctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGt > 1:973350/1‑150 (MQ=255) aTAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACggg > 2:1110138/1‑150 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTTCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc > 2:298270/1‑115 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc < 1:298270/115‑1 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt < 2:230213/150‑1 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt > 2:367234/1‑150 (MQ=255) tAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAAAAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt > 2:1249542/1‑150 (MQ=255) aGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTg < 1:229601/150‑1 (MQ=255) cgcTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGaa < 2:1101696/150‑1 (MQ=255) gCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAAtt > 1:921964/1‑120 (MQ=255) gCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAAtt < 2:921964/120‑1 (MQ=255) gCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGtt > 1:251852/1‑150 (MQ=255) tACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTTAGCATCGGCCCCGCATCATGGGCCTGCtgaaatgaaa < 1:1155714/96‑1 (MQ=255) tACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaatgaaa > 2:1155714/1‑96 (MQ=255) cAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAagc < 2:973350/150‑2 (MQ=255) caaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAagag > 1:984732/2‑150 (MQ=255) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTc > 2:346296/1‑144 (MQ=255) aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTc < 1:346296/144‑1 (MQ=255) ggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGa > 2:410392/1‑150 (MQ=255) | GAGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGA > NC_002947/1785919‑1786210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |