Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,808,650 C→G 8.1% T220S (ACT→AGT)  kdsA‑I → 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,808,6500CG 8.1% 148.7 / 5.1 50T220S (ACT→AGT) kdsA‑I2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base G (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

GCTTCGGCTACAACAACCTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAATTGGACG  >  NC_002947/1808503‑1808799
                                                                                                                                                       |                                                                                                                                                     
gCTTCGGCTACAACAACCTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTg                                                                                                                                                     >  2:1174403/1‑150 (MQ=255)
        tACAACAACCTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGcc                                                                                                                                             >  1:704466/1‑150 (MQ=255)
         acaacaACCTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCAGTGACCTgggcc                                                                                                                                            <  2:1291809/150‑4 (MQ=255)
                ccTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCACAGGTCACTGACCTGGCCAAGGCCgg                                                                                                                                     >  1:1103109/1‑150 (MQ=255)
                   ggTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCACAGGTCACTGACCTGGCCAAGGCCGGCAt                                                                                                                                  <  2:1103109/150‑1 (MQ=255)
                        tCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTc                                                                                                                             >  1:1201458/1‑150 (MQ=255)
                           aCATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAgg                                                                                                                          <  1:1174403/150‑1 (MQ=255)
                           aCATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAgg                                                                                                                          >  2:546183/1‑150 (MQ=255)
                                 tGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTgg                                                                                                                    >  2:1116263/1‑150 (MQ=255)
                                      ttCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCggg                                                                                                               >  1:1376822/1‑150 (MQ=255)
                                         ggCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCtt                                                                                                            >  2:1397837/1‑150 (MQ=255)
                                          gCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCttt                                                                                                           <  2:1201458/150‑1 (MQ=255)
                                           catcatGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCtttt                                                                                                          >  1:390121/1‑150 (MQ=255)
                                           catcatGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCtttt                                                                                                          >  1:390158/1‑150 (MQ=255)
                                                 aaaGCAGTTCGAGTCCCCGGTGGTCTTCGACTTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑ACCCATTGCACTGACCTGGCCAACGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGa                                                                                                    <  2:410281/149‑1 (MQ=255)
                                                    gCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGc                                                                                                 >  1:1253134/1‑150 (MQ=255)
                                                      aGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGAAATCGGAAGAGCACACGT‑‑CTGAACT‑‑CCAGTGCCTTCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGccc                                                                                               >  1:168026/1‑150 (MQ=255)
                                                       gTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCa                                                                                              <  1:394893/150‑1 (MQ=255)
                                                        ttCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGccccc                                                                                             >  1:1051717/1‑148 (MQ=255)
                                                           gAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCAcccc                                                                                          <  2:1237142/150‑1 (MQ=255)
                                                                 ccGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCg                                                                                    >  1:250052/1‑150 (MQ=255)
                                                                              aCGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGt                                                                       <  1:1050710/150‑1 (MQ=255)
                                                                                gTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGt                                                                                                                              <  2:939320/93‑1 (MQ=255)
                                                                                gTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGt                                                                                                                              >  1:939320/1‑93 (MQ=255)
                                                                                    cccACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATg                                                                 <  2:1253134/150‑1 (MQ=255)
                                                                                     ccACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATgg                                                                >  1:915582/1‑150 (MQ=255)
                                                                                     ccACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGA‑‑‑‑GCCCAGGAAAATAAAATGTCCAAGGAAGGCATGAGTCATTGCCTTGCCGGTCTTTATTTTGAGGCCCTCCCCGACCCCTACAACGCCAAGTGCGACgg                                                                >  2:1365948/1‑150 (MQ=255)
                                                                                         gCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCa                                                            <  2:390158/150‑1 (MQ=255)
                                                                                         gCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCa                                                            <  2:390121/150‑1 (MQ=255)
                                                                                          cccTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGACCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCAt                                                           <  1:14137/150‑1 (MQ=255)
                                                                                                        ggTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTg                                             <  2:569962/150‑1 (MQ=255)
                                                                                                          tGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGa                                           <  2:468770/150‑1 (MQ=255)
                                                                                                               gcgcCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAAc                                      >  2:1359368/1‑150 (MQ=255)
                                                                                                                 gcCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTgcg                                                 <  1:282091/137‑1 (MQ=255)
                                                                                                                 gcCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTgcg                                                 >  2:282091/1‑137 (MQ=255)
                                                                                                                 gcCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTg                                    >  1:790162/1‑150 (MQ=255)
                                                                                                                   cGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGa                                  <  2:704466/150‑1 (MQ=255)
                                                                                                                                 gtcgtcGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTcc                    >  1:1280199/1‑150 (MQ=255)
                                                                                                                                    gtcgG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGa                                                                                  <  2:975983/85‑1 (MQ=255)
                                                                                                                                    gtcgG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGa                                                                                  >  1:975983/1‑85 (MQ=255)
                                                                                                                                           ttggccAGGTCAGTGACCTGGGCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCa              >  2:1303203/5‑150 (MQ=255)
                                                                                                                                           ttggccAGGTCAGTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCt                                              <  1:8086/114‑1 (MQ=255)
                                                                                                                                           ttggccAGGTCAGTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCt                                              >  2:8086/5‑118 (MQ=255)
                                                                                                                                               ccAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCa          >  2:1304838/1‑150 (MQ=255)
                                                                                                                                                cAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCaa         >  2:889405/1‑150 (MQ=255)
                                                                                                                                                  ggTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAAtt       <  1:546183/150‑1 (MQ=255)
                                                                                                                                                  ggTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAAtt       <  1:1304838/150‑1 (MQ=255)
                                                                                                                                                     cACTGACCTGGCCAAGGCCGGCTGGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCGCCGCAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAATTGga    <  1:798513/150‑1 (MQ=255)
                                                                                                                                                      aCTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCaa             <  2:1071866/140‑1 (MQ=255)
                                                                                                                                                      aCTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCaa             >  1:1071866/1‑140 (MQ=255)
                                                                                                                                                       ctggcctggcCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAATTGgacg  >  1:463415/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GCTTCGGCTACAACAACCTGGTTGTCGACATGCTGGGCTTCGGCATCATGAAGCAGTTCGAGTACCCGGTGTTCTTCGACGTGACCCACGCCCTGCAGATGCCGGGTGGCCGCGCCGATTCCGCCGGCGGTCGTCGG‑‑‑‑GCCCAGGTCACTGACCTGGCCAAGGCCGGCATGAGTCAGGGCCTGGCCGGGCTTTTCCTCGAGGCCCACCCCGACCCGGACAACGCCAAGTGCGATGGCCCATGCGCCCTGCGCCTGGATAAACTGGAGCCGTTCCTGGTCCAGCTCAAGCAATTGGACG  >  NC_002947/1808503‑1808799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑