Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,810,378	C→A	10.8%	intergenic (+97/‑72)	eno → / → ftsB	enolase/cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,810,378	0	C	A	10.8%	189.9 / 10.6	65	intergenic (+97/‑72)	eno/ftsB	enolase/cell division protein
Reads supporting (aligned to +/- strand):  ref base C (25/33);  new base A (2/5);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

TGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGG  >  NC_002947/1810235‑1810523                                                                                                                                                |                                                                                                                                                  tGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAg                                                                                                                                             >  1:127941/1‑150 (MQ=255)   ggCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGc                                                                                                                                           <  2:655926/150‑1 (MQ=255)   ggCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGc                                                                                                                                           <  2:72571/150‑1 (MQ=255)    gcgcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCt                                                                                                                                          <  1:1268792/150‑1 (MQ=255)      gcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTc                                                                                                                                        <  2:841149/150‑1 (MQ=255)      gcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGCTTc                                                                                                                                        <  1:218771/150‑1 (MQ=255)      gcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGCTTc                                                                                                                                        <  2:1221589/150‑1 (MQ=255)        cAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGCTTCGt                                                                                                                                      <  1:371333/150‑1 (MQ=255)           aGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCt                                                                                                                                   >  2:454589/1‑150 (MQ=255)                               ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAc                                                                                                               <  1:1240192/150‑1 (MQ=255)                               ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAc                                                                                                               >  2:234819/1‑150 (MQ=255)                                      tCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGCGTTGCAGCCACGTTCGTACTGAACTTTCCTTCGTACTAACGGGTTTCTGTCGACGAAACCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTAcg                                                                                                        >  1:739412/1‑150 (MQ=255)                                           gCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCa                                                                                                   <  2:1369262/150‑1 (MQ=255)                                                gCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtt                                                                                              <  2:168746/150‑1 (MQ=255)                                                 cAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTa                                                                                                          >  1:108567/1‑137 (MQ=255)                                                 cAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTa                                                                                                          <  2:108567/137‑1 (MQ=255)                                                 cAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCttt                                                                                             <  1:470266/150‑1 (MQ=255)                                                 cAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCttt                                                                                             >  1:1337564/1‑150 (MQ=255)                                                  aaGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtttt                                                                                            >  2:1181088/1‑150 (MQ=255)                                                     agaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGc                                                                                                 >  1:1052969/1‑142 (MQ=255)                                                     agaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGc                                                                                                 <  2:1052969/142‑1 (MQ=255)                                                      gaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTATGTACTAACGGGTTTCTGTCGACCAATCCTGGCCTCGGCGATGCTTCGTGCTATTCGAGGCGCCGAAGTAACCAGTACGCGGCAGCCTTTTTAAc                                                                                        <  1:677479/150‑1 (MQ=255)                                                       aTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGaa                                                                                                                    <  1:732869/121‑1 (MQ=255)                                                       aTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGaa                                                                                                                    >  2:732869/1‑121 (MQ=255)                                                        tGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                               <  1:1209401/109‑1 (MQ=255)                                                        tGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                               >  2:1209401/1‑109 (MQ=255)                                                         ggTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTacc                                                                                        >  1:759444/1‑145 (MQ=255)                                                         ggTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTacc                                                                                        <  2:759444/147‑3 (MQ=255)                                                               aaGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAAc                                                                               <  2:457753/150‑1 (MQ=255)                                                                aGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACg                                                                              <  2:525276/150‑1 (MQ=255)                                                                       agcCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTCTCGTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAAAGTGATgcg                                                                       <  1:799394/150‑1 (MQ=255)                                                                              tacgTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTccc                                                                >  1:751223/4‑150 (MQ=255)                                                                                      aaCGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgtt                                                        <  1:485436/150‑1 (MQ=255)                                                                                                ctgtaatttCCTACGTACGAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGtt                                              <  1:201813/144‑1 (MQ=255)                                                                                                cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGACTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGtt                                              <  2:300019/150‑1 (MQ=255)                                                                                                 tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACg                                                                              >  2:85658/1‑117 (MQ=255)                                                                                                 tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACg                                                                              <  1:85658/117‑1 (MQ=255)                                                                                                 tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTa                                                              <  2:547662/133‑1 (MQ=255)                                                                                                 tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTa                                                              >  1:547662/1‑133 (MQ=255)                                                                                                   aaCTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGCTGCGCAGTCCCTATTGGTTGTTCCTCGTTCTg                                           <  2:127941/150‑1 (MQ=255)                                                                                                    aCTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGc                                          >  1:149768/1‑150 (MQ=255)                                                                                                       ttCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGctct                                       <  2:1337564/150‑1 (MQ=255)                                                                                                        tCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCtt                                      <  2:149768/150‑1 (MQ=255)                                                                                                          cTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGc                                    >  2:860616/1‑150 (MQ=255)                                                                                                          cTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGc                                    <  1:1181088/150‑1 (MQ=255)                                                                                                              gTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                                <  2:751223/150‑1 (MQ=255)                                                                                                               tACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                               >  2:462430/1‑150 (MQ=255)                                                                                                                aCTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTgg                              <  1:462430/150‑1 (MQ=255)                                                                                                                  tAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACaa                                                                                                             <  2:814370/69‑1 (MQ=255)                                                                                                                  tAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACaa                                                                                                             >  1:814370/1‑69 (MQ=255)                                                                                                                   aaCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTg                           >  2:1299479/1‑150 (MQ=255)                                                                                                                    aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTgg                          <  1:454589/150‑1 (MQ=255)                                                                                                                        gTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTg                      >  1:986445/1‑150 (MQ=255)                                                                                                                        gTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTg                      <  1:234819/150‑1 (MQ=255)                                                                                                                             tGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTAGTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTa                 >  1:1154394/1‑150 (MQ=255)                                                                                                                                  acgaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgtt                                                        >  2:565593/1‑106 (MQ=255)                                                                                                                                  acgaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgtt                                                        <  1:565593/106‑1 (MQ=255)                                                                                                                                    gaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtg          >  1:474925/1‑150 (MQ=255)                                                                                                                                     aaGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtgt         >  1:1081668/1‑150 (MQ=255)                                                                                                                                       gCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCa                    >  2:102990/1‑137 (MQ=255)                                                                                                                                       gCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCa                    <  1:102990/137‑1 (MQ=255)                                                                                                                                           ggCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgggtgg   <  1:48369/150‑1 (MQ=255)                                                                                                                                            gCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTc                                             >  2:57839/1‑107 (MQ=255)                                                                                                                                            gCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTc                                             <  1:57839/107‑1 (MQ=255)                                                                                                                                            gCCTCGACCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGtgggtggg  >  2:635116/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                  TGGGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGG  >  NC_002947/1810235‑1810523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑