Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,854,598 G→A 7.3% A140T (GCC→ACC)  PP_1658 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,854,5980GA 7.3% 156.7 / 2.9 55A140T (GCC→ACC) PP_1658hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base A (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CACCGAGGATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCTTGGC  >  NC_002947/1854459‑1854741
                                                                                                                                           |                                                                                                                                               
cACCGAGGATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGaa                                                                                                                                       <  2:507729/150‑1 (MQ=255)
 aCCGAGGATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAg                                                                                                                                      >  1:846202/1‑150 (MQ=255)
    gAGGATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATc                                                                                                                                   <  2:770842/150‑1 (MQ=255)
       gATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAg                                                                                                                                <  2:1179196/150‑1 (MQ=255)
        aTTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCCACGCGCTACTGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCCgg                                                                                                                               >  2:195038/1‑150 (MQ=255)
           actacAACTTCGTCGACGACCAGACGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGGGGTTATCCCCCGTGAGGCCGCATTGAAGGGCCAGGAgc                                                                                                                            <  2:945368/150‑1 (MQ=255)
               cAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGa                                                                                                                        >  1:567016/1‑150 (MQ=255)
                 aCTTCGTCGACGCCAACATGGTCTATCGCATTTCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAAGAGCACGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGAcc                                                                                                                      <  1:1380512/150‑1 (MQ=255)
                            gacAAGAAGTTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGGCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCGAGGAGCGCGACCCTGACCGTAtt                                                                                                           >  2:133441/1‑150 (MQ=255)
                                 gaagGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCAt                                                                                                                                           <  1:1124349/113‑1 (MQ=255)
                                 gaagGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCAt                                                                                                                                           >  2:1124349/1‑113 (MQ=255)
                                 gaagGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAgcg                                                                                                                           <  1:1426632/129‑1 (MQ=255)
                                 gaagGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAgcg                                                                                                                           >  2:1426632/1‑129 (MQ=255)
                                     gTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCa                                                                                                                                 <  2:478600/119‑1 (MQ=255)
                                     gTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCa                                                                                                                                 >  1:478600/1‑119 (MQ=255)
                                          gcgcATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTctgct                                                                                                      <  2:876223/141‑1 (MQ=255)
                                          gcgcATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTctgct                                                                                                      >  1:876223/1‑141 (MQ=255)
                                                   cGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGa                                                                                    <  1:540752/150‑1 (MQ=255)
                                                    gTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAg                                                                                   <  2:846202/150‑1 (MQ=255)
                                                    gTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGACCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAg                                                                                   <  2:1051105/150‑1 (MQ=255)
                                                    gTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGACCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAg                                                                                   <  2:1050836/150‑1 (MQ=255)
                                                    gTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGACCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAg                                                                                   >  1:1050836/1‑150 (MQ=255)
                                                    gTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGACCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAg                                                                                   >  1:1051105/1‑150 (MQ=255)
                                                     tCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAgg                                                                                  >  1:414980/1‑150 (MQ=255)
                                                       aaCGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGCTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGaa                                                                                >  2:333626/1‑150 (MQ=255)
                                                        aCGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGaaa                                                                               >  2:308259/1‑150 (MQ=255)
                                                         cgcgCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAAc                                                                              <  1:993623/150‑1 (MQ=255)
                                                              tGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGGGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATACAGGAGCGCGACCCTGGCCGTATTCGGCTGCTCAACAGCCATGTCGAGGAAACGGACg                                                                         >  1:590571/1‑150 (MQ=255)
                                                                  gcgcACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTAtt                                                                                                           >  2:456455/1‑112 (MQ=255)
                                                                  gcgcACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTAtt                                                                                                           <  1:456455/112‑1 (MQ=255)
                                                                  gcgcACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAga                                                                     <  2:567016/150‑1 (MQ=255)
                                                                       ccAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTc                                                                                                          >  1:724582/1‑108 (MQ=255)
                                                                       ccAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTc                                                                                                          <  2:724582/108‑1 (MQ=255)
                                                                       ccAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGAcc                                                                <  1:308259/150‑1 (MQ=255)
                                                                        cAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGAccc                                                               >  2:1049400/1‑150 (MQ=255)
                                                                             tGAGCAACGGCGCGCTGGCCGTGGTAACCGATTCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTAcg                                                          >  1:749934/1‑150 (MQ=255)
                                                                                  aaCGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCt                                                     >  1:137927/1‑150 (MQ=255)
                                                                                          gcTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGAt                                             >  1:651297/1‑150 (MQ=255)
                                                                                            tGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATcc                                           <  1:1049400/150‑1 (MQ=255)
                                                                                                   ggTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCtgatga                                    <  2:1078989/150‑1 (MQ=255)
                                                                                                    gTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTgatgat                                   >  1:280592/1‑150 (MQ=255)
                                                                                                         ccATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGAt                              >  1:663471/1‑150 (MQ=255)
                                                                                                         ccATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGAt                              >  2:1324348/1‑150 (MQ=255)
                                                                                                          cATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGAtg                             >  1:359297/1‑150 (MQ=255)
                                                                                                              ccGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGt                         <  2:651297/150‑1 (MQ=255)
                                                                                                                ggtggtTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTaa                       >  2:803205/1‑150 (MQ=255)
                                                                                                                     ttACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACg                  >  2:1097535/1‑150 (MQ=255)
                                                                                                                         gAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGaa                >  2:1186689/1‑148 (MQ=255)
                                                                                                                         gAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGaa                <  1:1186689/148‑1 (MQ=255)
                                                                                                                                  ccccGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACaa                                                >  2:116753/1‑107 (MQ=255)
                                                                                                                                  ccccGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACaa                                                <  1:116753/107‑1 (MQ=255)
                                                                                                                                  ccccGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCtt     >  2:1328078/1‑150 (MQ=255)
                                                                                                                                  caccGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCtt     >  2:141141/3‑150 (MQ=255)
                                                                                                                                     cGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCTTGGc  >  2:740297/1‑150 (MQ=255)
                                                                                                                                     cGTGAGGACGCATTGACGATCCAAGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCAGGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCTTGGc  <  2:359297/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CACCGAGGATTACTACAACTTCGTCGACGACAAGAAGGTCAAGCGCATTGCCGTCAACGCGCTGATGCGCACCAAGCTGAGCAACGGCGCGCTGGCCGTGGTAGCCCATGCCGGTGGTTACGAGGTTATCCCCCGTGAGGCCGCATTGAAGATCCAGGAGCGCGACCCTGGCCGTATTCTGCTGCTCAACAGCCATGTCGAGGAAACGGACGAAGACGACCCGTACGCGGCTTACAAGATCCCTGATGATCTGATGTGGTAAGCACGAAAATCCCGCCTTGGC  >  NC_002947/1854459‑1854741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑