Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,859,162 C→G 9.4% intergenic (+40/+61) PP_1661 → / ← PP_1662 dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,859,1620CG 9.4% 130.3 / 4.9 44intergenic (+40/+61)PP_1661/PP_1662dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/17);  new base G (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTT  >  NC_002947/1859015‑1859311
                                                                                                                                                   |                                                                                                                                                     
ggTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCggag                                                                                                                                                     >  2:649872/1‑150 (MQ=255)
      ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTCTCATGACAACCCCACGATGCTCCGGCAATGCCGCAGCAtttt                                                                                                                                               >  1:441979/1‑150 (MQ=255)
         cAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTggg                                                                                                                                            <  2:810610/150‑1 (MQ=255)
          aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCGGAGCAtcgtgggg                                                                                                                                           <  1:649872/150‑8 (MQ=38)
            cTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGACAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGtt                                                                                                                                         >  1:546608/1‑150 (MQ=255)
              gtgCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGc                                                                                                                                       >  1:69364/1‑150 (MQ=255)
                gCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGcca                                                                                                                                     <  2:1036895/150‑3 (MQ=38)
                     aaTGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGa                                                                                                                                <  2:69364/150‑1 (MQ=255)
                     aaTGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGa                                                                                                                                <  1:612108/150‑1 (MQ=255)
                       tGACGACCTGGACGGGCGTTACGGGGCTGGACTGAGCCATAACAAGAGCAGAGTGTCGGGTGATATGTGGTTCTTTGGGTTGGAGGCCCGTTGATTGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGc                                                                                                                              >  2:162493/1‑150 (MQ=255)
                                  aCGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTc                                                                                                                   >  1:59844/1‑150 (MQ=255)
                                      gCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAggg                                                                                                               >  2:390011/1‑150 (MQ=255)
                                                      cTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGc                                                                                               >  1:610799/1‑150 (MQ=255)
                                                       tttgCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCa                                                                                              >  1:410247/4‑150 (MQ=255)
                                                       tGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCa                                                                                              >  1:215605/1‑150 (MQ=255)
                                                       tGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCa                                                                                              >  1:110140/1‑150 (MQ=255)
                                                            gATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGACGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGGGTCAGGGGTTCGCCGCAATGGGCAgggac                                                                                         >  2:303760/1‑148 (MQ=255)
                                                                  cTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtg                                                                                   >  1:181723/1‑150 (MQ=255)
                                                                    gggCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgtgtg                                                                                 <  1:564970/150‑1 (MQ=255)
                                                                                   agagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTcc                                                                  <  1:390011/150‑1 (MQ=255)
                                                                                    gagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCa                                                                 <  2:59844/150‑1 (MQ=255)
                                                                                       gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAg                                                                                                                 <  2:97864/99‑1 (MQ=255)
                                                                                       gTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAg                                                                                                                 >  1:97864/1‑99 (MQ=255)
                                                                                            tttCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCa                                                         >  2:554170/1‑150 (MQ=255)
                                                                                             ttCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCaa                                                        <  1:29927/150‑1 (MQ=255)
                                                                                              tCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAg                                                       <  2:545152/150‑1 (MQ=255)
                                                                                                        gtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAgg                                                                                            >  1:1219125/1‑103 (MQ=255)
                                                                                                        gtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAgg                                                                                            <  2:1219125/103‑1 (MQ=255)
                                                                                                             cccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAgg                                                                                            <  1:1068925/98‑1 (MQ=255)
                                                                                                             cccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAgg                                                                                            >  2:1068925/1‑98 (MQ=255)
                                                                                                              ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTgg                                       >  1:1127244/1‑150 (MQ=255)
                                                                                                              ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTgg                                       >  1:295061/1‑150 (MQ=255)
                                                                                                                 gTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgtgt                                    <  2:1165251/150‑1 (MQ=255)
                                                                                                                      agCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                               >  1:1341025/2‑150 (MQ=37)
                                                                                                                           ccccaaaaTGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                          >  2:814138/8‑150 (MQ=38)
                                                                                                                           ccccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt                          <  2:295061/150‑1 (MQ=255)
                                                                                                                            cccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                         >  2:1054508/1‑150 (MQ=255)
                                                                                                                            cccACGATGCTCCGGAAATGCCGTACCAGTTTGGGGTTGTTCACTGAGACCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTTAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTc                         <  2:582263/150‑1 (MQ=255)
                                                                                                                             ccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                               >  2:1401064/1‑143 (MQ=255)
                                                                                                                             ccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc                               <  1:1401064/143‑1 (MQ=255)
                                                                                                                              cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTgg                                                                                <  2:1375529/93‑1 (MQ=255)
                                                                                                                              cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTgg                                                                                >  1:1375529/1‑93 (MQ=255)
                                                                                                                                           cAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCACTGGGCAGGGTAATTGAGCGGCGCGAGCGTTTTCCAGTTGTTCAAGCTCGACTCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGcc          <  2:1230913/150‑1 (MQ=255)
                                                                                                                                                 cGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAg    <  2:110140/150‑1 (MQ=255)
                                                                                                                                                 cGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAg    >  1:991581/1‑150 (MQ=255)
                                                                                                                                                  gcagcaTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGt   <  1:162493/150‑1 (MQ=255)
                                                                                                                                                   cagcaTTTTGGGGTTGCTCCCTGAGCCCAGGCTAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCACCGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGtt  <  1:137995/150‑1 (MQ=255)
                                                                                                                                                   cagcaTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGtt  <  2:181723/150‑1 (MQ=255)
                                                                                                                                                   cagcaTTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGtt  <  1:554170/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCGTTATGGAACATGCCCACCAGTT  >  NC_002947/1859015‑1859311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑