Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,909,160 G→A 12.7% Q45* (CAG→TAG)  PP_1709 ← fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,909,1600GA12.7% 132.1 / 9.3 55Q45* (CAG→TAG) PP_1709fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base A (5/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

TCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCT  >  NC_002947/1909015‑1909306
                                                                                                                                                  |                                                                                                                                                   
tCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTgcggc                                                                                                                                                 >  2:800982/1‑150 (MQ=255)
tCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTgcggc                                                                                                                                                 >  2:676469/1‑150 (MQ=255)
        gctGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGgcgccgcgcc                                                                                                                                         >  2:1200653/3‑150 (MQ=255)
        gATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGgcgccgcgcc                                                                                                                                         >  2:455395/1‑150 (MQ=255)
              ggCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCGGCGCCGCAGGa                                                                                                                                   <  1:859182/150‑1 (MQ=255)
                   cgccTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCg                                                                                                                              <  1:800982/150‑1 (MQ=255)
                     ccTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGc                                                                                                                            >  1:655141/1‑150 (MQ=255)
                       tttGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCGGCGCCGCAGGa                                                                                                                                   <  2:48792/141‑1 (MQ=255)
                       tttGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCGGCGCCGCAGGa                                                                                                                                   >  1:48792/1‑141 (MQ=255)
                           gCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACggg                                                                                                                      <  2:57364/150‑1 (MQ=255)
                               tGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGt                                                                                                                  >  1:777892/1‑150 (MQ=255)
                                   cgcgACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCg                                                                                                              >  2:499183/1‑150 (MQ=255)
                                   cgcgACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCg                                                                                                              <  2:655141/150‑1 (MQ=255)
                                    gcgACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCgg                                                                                                             >  1:849552/1‑150 (MQ=255)
                                          aGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAgg                                                                                                       <  1:676469/150‑1 (MQ=255)
                                            cTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCa                                                                                                     <  1:1098806/150‑1 (MQ=255)
                                             tCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCCCGTTGCTTGCCGGTTTCATGAAAAACCCAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGccg                                                                                                    >  2:202294/1‑148 (MQ=255)
                                                        gCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGcc                                                                                        >  1:1364193/1‑150 (MQ=255)
                                                              cTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCgg                                                                                  <  2:945922/150‑1 (MQ=255)
                                                               tcgtcgGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAg                                                                                                        <  1:872122/128‑1 (MQ=255)
                                                               tcgtcgGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCggg                                                                                 >  1:1038015/1‑150 (MQ=255)
                                                               tcgtcgGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAg                                                                                                        >  2:872122/1‑128 (MQ=255)
                                                                  tcgGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAg                                                                              <  2:494965/150‑1 (MQ=255)
                                                                   cgGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGTATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAgc                                                                             <  1:175642/150‑1 (MQ=255)
                                                                                    ggCAGTTCGCCCAGTGCCTCGACCACTTTGCTTGCCGGTTTCATGAAAAACACCAAGCCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACGCCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCgg                                                            >  1:886386/1‑150 (MQ=255)
                                                                                     gCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGa                                                           <  2:777892/150‑1 (MQ=255)
                                                                                     gCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGCTTAATGAAACCAACGCGCTCCTGCGGCGCCGCGCCGTAGGATTACGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGa                                                           >  1:1096857/1‑150 (MQ=255)
                                                                                            gCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAg                                                    <  2:29989/150‑1 (MQ=255)
                                                                                              ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCa                                                                                                     <  1:1094457/100‑1 (MQ=255)
                                                                                              ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCa                                                                                                     >  2:1094457/1‑100 (MQ=255)
                                                                                                     cTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGAt                                           >  2:347640/1‑150 (MQ=255)
                                                                                                     cTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGAt                                           >  2:1271716/1‑150 (MQ=255)
                                                                                                      tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                          <  2:1096857/150‑1 (MQ=255)
                                                                                                      tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                          <  2:849552/150‑1 (MQ=255)
                                                                                                             cGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCcggcg                                   <  1:1164827/150‑1 (MQ=255)
                                                                                                               ttgcttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCggg                                 >  1:1215953/1‑150 (MQ=255)
                                                                                                               ttgcttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCggg                                 <  1:1271716/150‑1 (MQ=255)
                                                                                                                   ttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGGGGCCCATCCGGGATGCCCTCCTGGGCAGCTCAGCGGTGCCGCGGATCGGCAGGCTGACGATAGCCGGGGGGGTGa                             >  1:525498/1‑150 (MQ=255)
                                                                                                                   ttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCg                                                        <  2:1018280/123‑1 (MQ=255)
                                                                                                                   ttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCg                                                        >  1:1018280/1‑123 (MQ=255)
                                                                                                                    tgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGaa                            <  1:1200653/150‑1 (MQ=255)
                                                                                                                       cGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACAcc                                                                                             >  2:167251/1‑82 (MQ=255)
                                                                                                                       cGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACAcc                                                                                             <  1:167251/82‑1 (MQ=255)
                                                                                                                           ttCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTaa                     <  2:188869/150‑1 (MQ=255)
                                                                                                                               tGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                          <  2:1413540/125‑1 (MQ=255)
                                                                                                                               tGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                          >  1:1413540/1‑125 (MQ=255)
                                                                                                                               tGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGtga                 >  1:1172274/1‑148 (MQ=255)
                                                                                                                               tGAAAAACACAGGCTCCTGCGGCGCCACGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCa                 >  1:831764/1‑150 (MQ=255)
                                                                                                                                 aaaaaCACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATg               >  2:232724/1‑150 (MQ=255)
                                                                                                                                     acacAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTg           <  1:499183/150‑1 (MQ=255)
                                                                                                                                       gccggaatCCTACGGCGCGGCGCCGCAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTgg          >  2:1184896/8‑150 (MQ=255)
                                                                                                                                            gCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcct     >  1:297358/1‑150 (MQ=255)
                                                                                                                                            aatCCTACGGCGCGGCGCCGCAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcct     >  1:1293549/3‑150 (MQ=255)
                                                                                                                                               ccTGCGGCGCCGCGCCGTAGGATTCCGGCACCGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATcctcct  <  2:337599/150‑1 (MQ=255)
                                                                                                                                               ccTACGGCGCGGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATcctcct  >  1:360228/1‑150 (MQ=255)
                                                                                                                                                 tGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACa                          >  2:278747/1‑124 (MQ=255)
                                                                                                                                                 tGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACa                          <  1:278747/124‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCT  >  NC_002947/1909015‑1909306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: