Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,933,717 A→C 9.5% L27R (CTC→CGC)  minD ← MinC‑MinD‑MinE system ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,933,7170AC 9.5% 161.9 / 9.6 63L27R (CTC→CGC) minDMinC‑MinD‑MinE system ATPase
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base C (3/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

CTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAG  >  NC_002947/1933569‑1933862
                                                                                                                                                    |                                                                                                                                                 
cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAg                                                                                                                                                  >  2:1165489/1‑150 (MQ=255)
 ttGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAgg                                                                                                                                                 >  1:1351431/1‑150 (MQ=255)
  tGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGc                                                                                                                                                <  2:31447/150‑1 (MQ=255)
     tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCgg                                                                                                                                             >  2:632513/1‑150 (MQ=255)
     tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCgg                                                                                                                                             <  2:1289494/150‑1 (MQ=255)
          gCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGccacaa                                                                                                                                           <  1:143006/147‑5 (MQ=255)
          gCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGccacaa                                                                                                                                           >  2:143006/1‑143 (MQ=255)
          gCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCa                                                                                                                                        >  2:1220599/1‑150 (MQ=255)
           ccTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGCCGGTCTTGTGGCCGCGCAGTGCGAGGCGGGTACaaa                                                                                                                                       >  2:688751/1‑150 (MQ=255)
                       tGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTgg                                                                                                                           <  1:1220599/150‑1 (MQ=255)
                       tGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTgg                                                                                                                           <  2:574002/150‑1 (MQ=255)
                        ggCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGt                                                                                                                          >  2:886715/1‑150 (MQ=255)
                         gCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTa                                                                                                                         <  1:842451/150‑1 (MQ=255)
                           ttCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGt                                                                                                                       >  2:564216/1‑150 (MQ=255)
                            tCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                      >  2:819241/1‑150 (MQ=255)
                              gCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGt                                                                                                                    <  1:1165489/150‑1 (MQ=255)
                                  ttGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTg                                                                                                                <  2:319878/150‑1 (MQ=255)
                                   ttaCCACGTTGCCGATGTCCTACACCACGCCGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGc                                                                                                               <  2:167938/148‑1 (MQ=255)
                                   tGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGc                                                                                                               <  1:1199386/150‑1 (MQ=255)
                                      ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCca                                                                                                            <  2:952961/150‑1 (MQ=255)
                                              aCGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACAcccccc                                                                                                    >  2:422180/1‑150 (MQ=255)
                                                gAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCtt                                                                                                  >  1:257586/1‑150 (MQ=255)
                                                      gTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                          <  2:471371/136‑1 (MQ=255)
                                                      gTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                          >  1:471371/1‑136 (MQ=255)
                                                          accacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTa                                                                                        <  2:257586/150‑1 (MQ=255)
                                                            cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAAc                                                                                      >  2:640812/1‑150 (MQ=255)
                                                            cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAAc                                                                                      >  2:436075/1‑150 (MQ=255)
                                                            cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAAc                                                                                      <  1:559945/150‑1 (MQ=255)
                                                               gcggcgTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCAc                                                                                   <  1:819241/150‑1 (MQ=255)
                                                                 ggcgTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACga                                                                                 <  1:632513/150‑1 (MQ=255)
                                                                        gCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCtt                                                                          <  1:436075/150‑1 (MQ=255)
                                                                          aGCCCATGATCAGGTGGAGGTTACTCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATTGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTgg                                                                        <  1:688751/150‑1 (MQ=255)
                                                                          aGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTgg                                                                        >  2:804044/1‑150 (MQ=255)
                                                                            cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGat                                                                                                                                                  <  1:49946/74‑2 (MQ=255)
                                                                            cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAg                                                                                                                                                  >  2:49946/1‑74 (MQ=255)
                                                                             ccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCa                                                                     >  2:909637/1‑150 (MQ=255)
                                                                              cATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCaa                                                                    <  1:886715/150‑1 (MQ=255)
                                                                                   tCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGATGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGgtgt                                                               <  2:1043249/150‑1 (MQ=255)
                                                                                       gTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTg                                                                                                                <  1:434624/97‑1 (MQ=255)
                                                                                       gTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTg                                                                                                                >  2:434624/1‑97 (MQ=255)
                                                                                        tCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAc                                                          >  2:1255893/1‑150 (MQ=255)
                                                                                          gAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                      <  1:450515/88‑1 (MQ=255)
                                                                                          gAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                      >  2:450515/1‑88 (MQ=255)
                                                                                           aGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAcccc                                                       <  1:422180/150‑1 (MQ=255)
                                                                                            ggTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCt                                                      >  1:894142/1‑150 (MQ=255)
                                                                                                     ggCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGt                                             <  1:804044/150‑1 (MQ=255)
                                                                                                       cccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCg                                           <  2:324158/150‑1 (MQ=255)
                                                                                                        ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                      >  1:651721/1‑74 (MQ=255)
                                                                                                        ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                                      <  2:651721/74‑1 (MQ=255)
                                                                                                        ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAg                                                                   >  1:136470/1‑125 (MQ=255)
                                                                                                        ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAg                                                                   <  2:136470/125‑1 (MQ=255)
                                                                                                        ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                          >  1:766943/1‑150 (MQ=255)
                                                                                                                     gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                           <  2:1361485/88‑1 (MQ=255)
                                                                                                                     gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                           >  1:1361485/1‑88 (MQ=255)
                                                                                                                     gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAACACTAGTCGGGACACTTATACCt                             >  2:441282/1‑150 (MQ=255)
                                                                                                                     gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCt                             <  1:640812/150‑1 (MQ=255)
                                                                                                                             cGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaaaaa                     <  2:1351431/150‑1 (MQ=255)
                                                                                                                                  ttGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaa                >  1:579836/1‑150 (MQ=255)
                                                                                                                                  ttGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaa                >  2:428116/1‑150 (MQ=255)
                                                                                                                                  taccggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAc                                                          <  1:455469/104‑1 (MQ=255)
                                                                                                                                  taccggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAc                                                          >  2:455469/5‑108 (MQ=255)
                                                                                                                                     cggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATa             >  2:356681/2‑150 (MQ=255)
                                                                                                                                           cgcAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCtaaa       <  1:441282/150‑1 (MQ=255)
                                                                                                                                                tGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAg  <  2:894142/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAAATAAAG  >  NC_002947/1933569‑1933862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑