| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,981,135 | T→G | 13.7% | intergenic (+31/‑269) | rpsA → / → ihfB | 30S ribosomal protein S1/integration host factor subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,981,135 | 0 | T | G | 13.7% | 96.7 / 11.6 | 44 | intergenic (+31/‑269) | rpsA/ihfB | 30S ribosomal protein S1/integration host factor subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (19/19); new base G (3/3); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01 | |||||||||||
GAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTC > NC_002947/1980990‑1981283 | gAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCggg > 1:1289020/1‑150 (MQ=255)gAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCggg < 1:721265/150‑1 (MQ=255) aaaGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGt < 2:1214434/150‑1 (MQ=255) gacgacGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGc > 2:900520/1‑150 (MQ=255) cgacgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCACGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCGAGGGTCGccc < 2:1034030/150‑1 (MQ=255) gacgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCt > 1:1306372/1‑150 (MQ=255) tGAAGAGCGCGGAGCTGGCCAGAGCCTGAAGAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtttttt > 2:800504/1‑150 (MQ=255) cgAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCCGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCcgc > 1:507643/1‑150 (MQ=255) tGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCcgc < 1:1149615/133‑1 (MQ=255) tGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCcgc > 2:1149615/1‑133 (MQ=255) tgctgcCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTgc > 1:432767/1‑127 (MQ=255) tgctgcCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTgc < 2:432767/127‑1 (MQ=255) ccGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAg > 1:1114981/1‑150 (MQ=255) gACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGcaacct > 2:97478/1‑145 (MQ=255) acacCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCtt < 2:1114981/150‑1 (MQ=255) aTGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCCTCACGCtcg < 2:1306372/150‑3 (MQ=255) tgcgctgcTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGATGGCGAAg < 2:133592/150‑1 (MQ=255) gcgAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCgg > 1:483952/1‑60 (MQ=255) gcgAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCgg < 2:483952/60‑1 (MQ=255) cgAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGa < 2:507643/150‑1 (MQ=255) gCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCgg < 2:1289020/150‑1 (MQ=255) tGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCCTGAAGCTGGc > 2:109014/1‑126 (MQ=255) aaGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCcg < 1:800504/150‑1 (MQ=255) cTGAGTTCTGTTTGATCGGTAACAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGGGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAg < 1:97478/150‑1 (MQ=255) ttCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgct < 2:679792/84‑1 (MQ=255) ttCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgct > 1:679792/1‑84 (MQ=255) ttCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGcc > 1:84652/1‑150 (MQ=255) tGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAg > 1:1356700/1‑139 (MQ=255) tGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAg < 2:1356700/139‑1 (MQ=255) gATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCt < 2:84652/150‑1 (MQ=255) cGGTAAAAAGGGCGACCCTCGTGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATc < 2:455543/150‑1 (MQ=255) gTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTa > 1:566962/1‑150 (MQ=255) aaaGGGCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATgg > 2:1173167/1‑60 (MQ=25) aaaGGGCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATgg < 1:1173167/60‑1 (MQ=25) aGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGt > 1:456567/1‑150 (MQ=255) aGGGCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGt > 1:796364/1‑150 (MQ=255) gggCGACCCTCGGGTCGCCCTTTTTTTTGCTGTGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTa > 1:37534/1‑150 (MQ=255) gggCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCgg < 2:266994/148‑1 (MQ=255) gggCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCgg > 1:266994/1‑148 (MQ=255) gggCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTa < 1:1398034/150‑1 (MQ=255) gggCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAg < 2:1232048/102‑1 (MQ=255) gggCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAg > 1:1232048/1‑102 (MQ=255) ccTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCAtt > 1:1373015/1‑150 (MQ=255) cTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGATCTGCTGTCGCGGAGCACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTc < 2:37534/150‑1 (MQ=255) | GAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTC > NC_002947/1980990‑1981283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |