Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,998,723 A→T 7.0% L267H (CTT→CAT)  rffG ← dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,998,7230AT 7.0% 181.5 / 4.4 57L267H (CTT→CAT) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base T (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

TCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTGC  >  NC_002947/1998576‑1998866
                                                                                                                                                   |                                                                                                                                               
tCGATTTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAgg                                                                                                                                               <  2:2646/150‑1 (MQ=255)
       tGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCaga                                                                                                                                        <  1:767967/150‑1 (MQ=255)
       tGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCaga                                                                                                                                        <  2:755851/150‑1 (MQ=255)
         cGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCCTACTGTTAGCCAGGCTCGCCGCTATTCTGATTTATTACCTGTCTCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAgt                                                                                                                                      <  2:162266/150‑1 (MQ=255)
          gTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAgtg                                                                                                                                     >  2:1387171/1‑150 (MQ=255)
           tGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAgtgt                                                                                                                                    <  2:937513/150‑1 (MQ=255)
             cATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTAc                                                                                                                                  <  1:230798/150‑1 (MQ=255)
                 gATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGcac                                                                                                                              >  2:713304/1‑150 (MQ=255)
                     gCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGAAGAGTATATGCAcgatg                                                                                                                          >  2:659453/1‑150 (MQ=255)
                      cATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaatgt                                                                                                                         >  2:478046/1‑150 (MQ=255)
                       aTAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca                                                                                                                                             <  1:1154381/129‑2 (MQ=255)
                       aTAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca                                                                                                                                             >  2:1154381/1‑128 (MQ=255)
                       aTAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaatgtc                                                                                                                        <  1:999619/150‑1 (MQ=255)
                             gcggTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGtt                                                                                                                  <  1:282747/150‑1 (MQ=255)
                                   aTGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCttt                                                                                                            <  2:617280/150‑1 (MQ=255)
                                      gCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACg                                                                                                                                 >  2:65220/1‑126 (MQ=255)
                                      gCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACg                                                                                                                                 <  1:65220/126‑1 (MQ=255)
                                                   gtTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTAc                                                                                                                                  <  2:1226451/112‑1 (MQ=255)
                                                   gtTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTAc                                                                                                                                  >  1:1226451/1‑112 (MQ=255)
                                                   gtTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCa                                                                                            >  2:82117/1‑150 (MQ=255)
                                                    ttACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCaa                                                                                           >  2:850610/1‑150 (MQ=255)
                                                    ttACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCaa                                                                                           >  2:869801/1‑150 (MQ=255)
                                                    ttACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTGCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCaa                                                                                           >  1:683834/1‑150 (MQ=255)
                                                           ggCGAGGAGGTGTGCATACTGGTCTACAGGCTCGCAGGTCTTCTTATTGATTCCCTTGCGCAATGCCGGACGTGCCATCTCGTCGAGAAGGCTGAAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGTCGCCAATTTTGTa                                                                                    <  2:99034/150‑1 (MQ=255)
                                                              gATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGt                                                                                 <  1:659453/150‑1 (MQ=255)
                                                                   gTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGCTGTCTCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGc                                                                            <  1:1086441/150‑1 (MQ=255)
                                                                    ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaatgtcaa                                                                                                                      >  2:1053095/1‑107 (MQ=255)
                                                                    ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaatgtcaa                                                                                                                      <  1:1053095/107‑1 (MQ=255)
                                                                       tGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGaa                                                                        >  2:641996/1‑150 (MQ=255)
                                                                                   gACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc                                                                           >  2:1074988/1‑135 (MQ=255)
                                                                                   gACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc                                                                           <  1:1074988/135‑1 (MQ=255)
                                                                                   gACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTc                                                            >  2:67532/1‑150 (MQ=255)
                                                                                     cAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCCGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCa                                                          <  1:1168001/150‑1 (MQ=255)
                                                                                     cAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCa                                                          <  1:587612/150‑1 (MQ=255)
                                                                                               ggTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACg                                                <  2:1159726/150‑1 (MQ=255)
                                                                                                 tcttctGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAg                                                                                                  <  2:211775/98‑1 (MQ=255)
                                                                                                 tcttctGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAg                                                                                                  >  1:211775/1‑98 (MQ=255)
                                                                                                       gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGt                                                                                 <  1:286107/109‑1 (MQ=255)
                                                                                                       gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGt                                                                                 >  2:286107/1‑109 (MQ=255)
                                                                                                             ttACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGc                                                                            >  1:305738/1‑108 (MQ=255)
                                                                                                             ttACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGc                                                                            <  2:305738/108‑1 (MQ=255)
                                                                                                                ccTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGAt                               <  1:869801/150‑1 (MQ=255)
                                                                                                                 cTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc                                                    <  1:1233421/128‑1 (MQ=255)
                                                                                                                 cTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc                                                    >  2:1233421/1‑128 (MQ=255)
                                                                                                                      gcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaat                                                                                                                           <  1:64471/52‑1 (MQ=255)
                                                                                                                      gcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCAcaat                                                                                                                           >  2:64471/1‑52 (MQ=255)
                                                                                                                              gCAGTTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGc                 <  1:713304/150‑1 (MQ=255)
                                                                                                                                      cATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGAGGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTcgcg         >  1:1302284/1‑150 (MQ=255)
                                                                                                                                       aTCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCgg        >  1:916663/1‑150 (MQ=255)
                                                                                                                                        tctcGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGa       <  1:641996/150‑1 (MQ=255)
                                                                                                                                          tcgtcgAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTTTTTTTTTTTTTTAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGCTCTTCGACCTACAGCCAGTAGCGCAtt     >  2:512456/1‑150 (MQ=255)
                                                                                                                                            gtcgAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGATTATACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTg   >  1:449597/1‑150 (MQ=255)
                                                                                                                                            gtcgAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTg   >  2:604272/1‑150 (MQ=255)
                                                                                                                                            gacgAGATGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCTACGGATGCCCGAGCAGt                                  >  1:231270/3‑119 (MQ=255)
                                                                                                                                            gacgAGATGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCTACGGATGCCCGAGCAGt                                  <  2:231270/117‑1 (MQ=255)
                                                                                                                                               gagaAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTGc  >  1:582254/1‑148 (MQ=255)
                                                                                                                                               gagaAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTGc  <  2:582254/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGGATTTGC  >  NC_002947/1998576‑1998866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑