| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,000,466 | G→T | 11.1% | V122F (GTT→TTT) | PP_1786 → | glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,000,466 | 0 | G | T | 11.1% | 150.2 / 8.2 | 54 | V122F (GTT→TTT) | PP_1786 | glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (26/22); new base T (2/4); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
CTCGGCTTCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAACGGGTTCAGCCTTTCAGCAGCCATTGA > NC_002947/2000318‑2000611 | cTCGGCTTCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGt < 1:1055996/150‑1 (MQ=255) ggCTTCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGa < 1:802737/150‑1 (MQ=255) gCTTCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGaa < 1:1317674/150‑1 (MQ=255) ttCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGaggt < 1:1160804/150‑4 (MQ=255) cACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCaa > 2:238644/1‑150 (MQ=255) aCGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGtt < 1:294274/140‑1 (MQ=255) aCGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAg > 2:335432/1‑150 (MQ=255) aCGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAg < 1:335432/150‑1 (MQ=255) aCGAAAGTGGTGATGCGGAGATTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGtt > 2:294274/1‑140 (MQ=255) cGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGcac < 1:549116/150‑1 (MQ=255) ggAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGcaca > 2:1041251/1‑150 (MQ=255) aTCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGag > 1:835806/1‑119 (MQ=255) aTCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGag < 2:835806/120‑2 (MQ=255) gCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGaa > 2:244239/1‑129 (MQ=255) gCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGaa < 1:244239/129‑1 (MQ=255) gCAGTTCTCAATGCGGCCGACAGCGCCGTTAAGCTCAAGACGGATGTTGCGAAACAGATCCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGa < 1:945362/150‑1 (MQ=255) cAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGag < 2:124024/110‑2 (MQ=255) cAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAACGCCGCCGGCTTTGag > 1:124024/1‑109 (MQ=255) gTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGTGGAAGCACATTTCCAACGCTTCGGACgg > 1:1266867/1‑150 (MQ=255) gTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACgg > 2:1217492/1‑150 (MQ=255) cAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGa < 2:468875/150‑1 (MQ=255) aaTGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAg < 1:379519/150‑1 (MQ=255) ggCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCg > 2:460830/1‑150 (MQ=255) ggCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGAAGGGCGGAGGGGCg > 1:298227/1‑150 (MQ=255) ccGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCa > 2:1164845/1‑124 (MQ=255) ccGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCa < 1:1164845/124‑1 (MQ=255) ccGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGAc > 2:39483/1‑150 (MQ=255) ccGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGAc < 2:430242/150‑1 (MQ=255) aCAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTc < 1:940053/150‑1 (MQ=255) aCAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTc < 1:39483/150‑1 (MQ=255) aCAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGACTTTTCACTTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCCAGCTGTGGCGGTAGCACATTTCTAACGCTTCGGACGGGCGGAGGGGCGACTTc > 1:747538/1‑150 (MQ=255) gcCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTAct > 2:481773/1‑150 (MQ=255) gTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACtctc < 1:238644/150‑1 (MQ=255) aaGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTgg > 2:973527/1‑150 (MQ=255) ccGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGCCCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGc < 1:914045/124‑1 (MQ=255) ccGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGc > 2:914045/1‑124 (MQ=255) cGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTa > 1:972830/1‑150 (MQ=255) gACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTGCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCa < 1:1041251/150‑1 (MQ=255) cTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGa < 2:440946/105‑1 (MQ=255) cTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGa > 1:440946/1‑105 (MQ=255) cTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTa > 1:1414101/1‑150 (MQ=255) tGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGc > 2:583284/1‑102 (MQ=255) tGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGc < 1:583284/102‑1 (MQ=255) aCCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTAc < 2:534808/150‑1 (MQ=255) tGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGa > 1:318041/1‑150 (MQ=255) tGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGa > 1:99342/1‑150 (MQ=255) gACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCa > 2:853015/1‑133 (MQ=255) gACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCa < 1:853015/133‑1 (MQ=255) gACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCt > 2:1396689/1‑141 (MQ=255) gACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCt < 1:1396689/141‑1 (MQ=255) aCCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAAc > 1:813817/1‑150 (MQ=255) ccTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGTGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCTATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAACg < 2:1266867/150‑1 (MQ=255) cccTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAACGGGTTCAg > 2:171674/1‑150 (MQ=255) cAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGACGCAGCCTTCTCACGATTGGGACGGGCGGAGGGGCGACGTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCAACTAGAGCTGTTACCGAACGGGTTCAGCCTTTcagc > 1:247917/1‑150 (MQ=255) cggcgTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAACGGGTTCAGCCTTTCAGCAGCCATTGa < 1:973527/150‑1 (MQ=255) | CTCGGCTTCGCACGAAAGTGGTGATGCGGAGCTTATCGCTGAAGCAGTTCTCAATGCGGCCGACAGCGCCGTTATGGTCAAGCCGGATGTTGCGAAAGTTGACCTGGATTTTTACCTGACCTTTTACCCTGACCTCAAAGCCGGCGGCGTTGAAACTCAAGCTATTGCGGAAGCACATTTCCAACGCTTCGGACGGGCGGAGGGGCGACTTCCTACTCTCGATGCCTGGGCTAAGAAGCACGAGCTTCCACTAGAGCTGTTACCGAACGGGTTCAGCCTTTCAGCAGCCATTGA > NC_002947/2000318‑2000611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |