Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,021,532 G→C 8.5% intergenic (+26/‑413) PP_1798 → / → gmd outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,021,5320GC 8.5% 154.8 / 2.8 52intergenic (+26/‑413)PP_1798/gmdouter membrane efflux protein/GDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (3/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATGG  >  NC_002947/2021384‑2021681
                                                                                                                                                    |                                                                                                                                                     
cTCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGc                                                                                                                                                      >  2:1277616/1‑150 (MQ=255)
 tCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCGGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCt                                                                                                                                                     >  1:1168678/1‑150 (MQ=255)
    aCCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGaa                                                                                                                                                  <  1:643061/150‑1 (MQ=255)
     ccGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAag                                                                                                                                                 >  2:358646/1‑150 (MQ=255)
           ggcgggcgAGTTGAATAAGGAACGTGTGGATAACGTCAAAGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGATGATCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGGCTGCGCTTCAGGGGCAAAagcgcagc                                                                                                                                           >  2:1003301/1‑150 (MQ=255)
           ggCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagcgcagc                                                                                                                                           <  2:1216852/150‑1 (MQ=255)
             cGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagcgcagcgc                                                                                                                                         <  2:855192/150‑1 (MQ=255)
              gggTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCGCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGcc                                                                                                                                        <  1:1325850/150‑1 (MQ=255)
                   aGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGAGAAGTTGTTCGCCTGAGCGGCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCa                                                                                                                                   <  1:1242866/150‑1 (MQ=255)
                     ttGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGt                                                                                                                                 <  2:790317/150‑1 (MQ=255)
                       gAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTTGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGa                                                                                                                               >  1:25245/1‑150 (MQ=255)
                       gAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagc                                                                                                                                                >  2:1409592/1‑133 (MQ=255)
                       gAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAagc                                                                                                                                                <  1:1409592/133‑1 (MQ=255)
                       gAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGa                                                                                                                               <  2:764145/150‑1 (MQ=255)
                        aaTCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGaa                                                                                                                              >  1:115206/1‑150 (MQ=255)
                          tCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAgc                                                                                                                            <  2:139785/150‑1 (MQ=255)
                            aGGAAAGTGTGGATACCGGCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGTTGAAGCGCAGCGCCAACCAGTGAAgcgc                                                                                                                          >  1:1046817/1‑150 (MQ=255)
                             ggAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGca                                                                                                                         <  2:25245/150‑1 (MQ=255)
                               aaaGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGcaca                                                                                                                       <  2:115206/150‑1 (MQ=255)
                                      ggATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCaa                                                                                                                <  1:55806/150‑1 (MQ=255)
                                             gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGcc                                                                                                         <  1:827400/150‑1 (MQ=255)
                                             gTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGcc                                                                                                         >  1:1397379/1‑150 (MQ=255)
                                                     cTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTccc                                                                                                 <  2:1175022/150‑1 (MQ=255)
                                                        gTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCACCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCttt                                                                                              >  1:511065/1‑150 (MQ=255)
                                                         ttGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCCGTGAAGCGCACAGTATCAATTTATCCCCGGTCCCtttt                                                                                             <  2:18599/150‑1 (MQ=255)
                                                                  gTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAgc                                                                                                                            <  1:722565/110‑1 (MQ=255)
                                                                  gTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAgc                                                                                                                            >  2:722565/1‑110 (MQ=255)
                                                                      cTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTccc                                                                                >  2:771975/1‑150 (MQ=255)
                                                                       tGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCa                                                                               >  2:493812/1‑150 (MQ=255)
                                                                             cAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCt                                                                                                <  2:1390449/127‑1 (MQ=255)
                                                                             cAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCt                                                                                                >  1:1390449/1‑127 (MQ=255)
                                                                                 cTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCa                                                                     <  1:358646/150‑1 (MQ=255)
                                                                                 cTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCa                                                                     <  2:377764/150‑1 (MQ=255)
                                                                                 cTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCa                                                                     <  2:511065/150‑1 (MQ=255)
                                                                                      cGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGGGTCAGGCGa                                                                >  2:968647/1‑150 (MQ=255)
                                                                                       ggTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAg                                                               <  2:1236769/150‑1 (MQ=255)
                                                                                           gCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGCGGTCa                                                           >  2:490580/1‑150 (MQ=255)
                                                                                             gAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGATCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAg                                                         >  1:1362543/1‑150 (MQ=255)
                                                                                                               gcCAGTCGCTAAAAGCGAGGGGGACTACGCTTCAGGGGCGGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCAACGTCCCTTTTCCGTCAAAATCCGTCCGCTGGATAGTCGACGTCAAGATGGTTTTGCTTCATCGa                                       >  2:312208/1‑150 (MQ=255)
                                                                                                                ccAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGAc                                      <  2:808739/150‑1 (MQ=255)
                                                                                                                 cAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATg                                                      <  1:1003705/133‑1 (MQ=255)
                                                                                                                 cAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATg                                                      >  2:1003705/1‑133 (MQ=255)
                                                                                                                    tctcTGTAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTa                                  >  2:20784/4‑150 (MQ=255)
                                                                                                                         gAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGc                                                                           >  2:164491/1‑104 (MQ=255)
                                                                                                                         gAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGc                                                                           <  1:164491/104‑1 (MQ=255)
                                                                                                                                       cTGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCgg                  <  2:505792/147‑1 (MQ=255)
                                                                                                                                       cTGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCgg                  >  1:505792/1‑147 (MQ=255)
                                                                                                                                        tGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGc                                                                  >  1:104936/1‑98 (MQ=255)
                                                                                                                                        tGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGc                                                                  <  2:104936/98‑1 (MQ=255)
                                                                                                                                        tGCGCTTCAGCCCCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCgg              >  2:1167291/1‑150 (MQ=255)
                                                                                                                                             ttCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgct         >  2:909215/1‑150 (MQ=255)
                                                                                                                                             ttCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGgct         >  2:995233/1‑150 (MQ=255)
                                                                                                                                                    gCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATgg  <  2:1397379/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTCAACCGTTGGGCGGGTGAGTTGAATCAGGAAAGTGTGGATAACGTCAATGTCTGGTTGAGCCCGGTGCCTGAGACCAGCCTGCCCGGTGGCGAGGTTGCTCGCCTGGGCGCCAGTCGCTGAAAGCGAGGGGGTCTGCGCTTCAGGGGCTGAAGCGCAGCGCCAACCAGTGAAGCGCACAGTATCAATTTAGCCCCGGTCCCTTTTGCGTCCTGTTCCCATTGCGTGTCAGGCGAGGTCAAGATGGTTTGGCTTCATCGACCTTAGCGCAGGCCGACCCGGGCGGTGGCTGCTATGG  >  NC_002947/2021384‑2021681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑