Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,095,713 G→A 6.2% L270L (CTG→TTG)  alaA ← glutamate‑pyruvate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,095,7130GA 6.2% 175.7 / 2.1 64L270L (CTG→TTG) alaAglutamate‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base A (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GATGTCGTTGAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAGCGAGGCGGTGCTG  >  NC_002947/2095564‑2095861
                                                                                                                                                     |                                                                                                                                                    
gATGTCGTTGAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAg                                                                                                                                                      >  1:1355057/1‑150 (MQ=255)
 aTGTCGTTGAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGc                                                                                                                                                     >  2:5954/1‑150 (MQ=255)
    tCGTTGAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATg                                                                                                                                                  >  1:969057/1‑150 (MQ=255)
     cGTTGAGCAGTTCGCAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGt                                                                                                                                                 <  2:275000/150‑1 (MQ=255)
         gAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGAt                                                                                                                                             <  1:5954/150‑1 (MQ=255)
                  gTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGAt                                                                                                                                    >  1:1305735/1‑150 (MQ=255)
                      gTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAg                                                                                                                                >  2:862200/1‑150 (MQ=255)
                          ggTTGCGCTGCTCCAGCAGGCGGCCAGGCAGTAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCtgt                                                                                                                            <  1:714476/150‑1 (MQ=255)
                           gTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCt                                                                                                                              <  2:1095185/147‑1 (MQ=255)
                           gTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCt                                                                                                                              >  1:1095185/1‑147 (MQ=255)
                            ttGCGCTGCTCCAGCAGGCCGCCCGGCGGCAGCACCAGGTCGTGGAGGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCtgtgt                                                                                                                          <  1:702107/150‑1 (MQ=255)
                                gctgctCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCAGGTCGATGCCTTCGATGTAGCTGTGTGCgt                                                                                                                      >  2:1302620/1‑150 (MQ=255)
                                 ctgctCCAGCAGTCGTCCCGTCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCgtg                                                                                                                     <  2:790648/150‑1 (MQ=255)
                                 ctgctCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCgtg                                                                                                                     <  1:1298974/150‑1 (MQ=255)
                                               ggcCCGGCTGCAGCACCAGTTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCgg                                                                                                       >  2:1090970/1‑150 (MQ=255)
                                                   cggcggCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAAt                                                                                                   <  1:621179/150‑1 (MQ=255)
                                                     gcggcAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGGCGGGCCATGACGTTGGCGCCAAAGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGa                                                                                                 <  1:101524/150‑1 (MQ=255)
                                                       ggcAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTCCTCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATc                                                                                               <  1:602514/150‑1 (MQ=255)
                                                        gcagcaCCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATca                                                                                              >  2:413031/1‑150 (MQ=255)
                                                             aCCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGAATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATcagccag                                                                                         >  2:120369/1‑150 (MQ=255)
                                                              ccAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATcagccagc                                                                                        <  1:1177626/150‑1 (MQ=255)
                                                                     gTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAgccagcca                                                                                      <  2:1319012/145‑1 (MQ=255)
                                                                     gTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAgccagcca                                                                                      >  1:1319012/1‑145 (MQ=255)
                                                                        gATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGaa                                                                              <  1:1090970/150‑1 (MQ=255)
                                                                         aTGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTACGCCATTCACATGGGGGTGTTTCGTTTGCCCGGAAATGATCAGCCAGCCAGAGCGGAAg                                                                             >  1:674160/1‑150 (MQ=255)
                                                                          tGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAACATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGaaa                                                                                                    >  1:1375455/1‑126 (MQ=255)
                                                                          tGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAACATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGaaa                                                                                                    <  2:1375455/126‑1 (MQ=255)
                                                                             tctGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCCTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCg                                                                         >  2:710306/1‑150 (MQ=255)
                                                                                  tAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCAcc                                                                    <  1:896521/150‑1 (MQ=255)
                                                                                      cgccCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGGACAGGCGGATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGt                                                                <  2:156830/150‑1 (MQ=255)
                                                                                      cgccCAGCGCGGTCTGGAGGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGt                                                                >  2:492353/1‑150 (MQ=255)
                                                                                       gccCAGCGCGGTCTGGATGGAATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTa                                                               <  1:120369/150‑1 (MQ=255)
                                                                                          cAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGa                                                            <  2:41999/150‑1 (MQ=255)
                                                                                                ggTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGCTACGACTTGGa                                                      >  1:694855/1‑150 (MQ=255)
                                                                                                  tCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTAGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCgg                                                                                <  1:1370437/122‑1 (MQ=255)
                                                                                                  tCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTAGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCgg                                                                                >  2:1370437/1‑122 (MQ=255)
                                                                                                      gATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGcc                                                >  2:1024312/1‑150 (MQ=255)
                                                                                                      gATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGcc                                                >  2:1116965/1‑150 (MQ=255)
                                                                                                        tGGCATGCTGGGCCGGCACGTTGGCGCAGAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGt                                              >  2:236794/1‑150 (MQ=255)
                                                                                                        tGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGt                                              >  2:381309/1‑150 (MQ=255)
                                                                                                          gCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTg                                            <  1:1116965/150‑1 (MQ=255)
                                                                                                           cATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCtgt                                                                                                                            >  1:1042013/1‑69 (MQ=255)
                                                                                                           cATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCtgt                                                                                                                            <  2:1042013/69‑1 (MQ=255)
                                                                                                             tGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAg                                         >  1:460141/1‑150 (MQ=255)
                                                                                                             tGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAg                                         <  1:381309/150‑1 (MQ=255)
                                                                                                             tGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAg                                         <  1:413031/150‑1 (MQ=255)
                                                                                                                 gggCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCa                                     <  2:1355057/150‑1 (MQ=255)
                                                                                                                 gggCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCa                                     >  2:652649/1‑150 (MQ=255)
                                                                                                                   gCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAgg                                   <  1:1302620/150‑1 (MQ=255)
                                                                                                                       gCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCACGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGcaca                               >  1:1240520/1‑150 (MQ=255)
                                                                                                                           gTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCAcagcag                           >  1:307153/1‑150 (MQ=255)
                                                                                                                           gTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCAcagcag                           >  2:738705/1‑150 (MQ=255)
                                                                                                                                  cacaGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGa                    <  2:1368885/150‑1 (MQ=255)
                                                                                                                                  cacaGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGa                    <  2:969057/150‑1 (MQ=255)
                                                                                                                                      ggCGCATGTTGGCCAACATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGa                                                      >  1:818856/1‑112 (MQ=255)
                                                                                                                                      ggCGCATGTTGGCCAACATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGa                                                      <  2:818856/112‑1 (MQ=255)
                                                                                                                                       gcgcATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGCAGTCCGCCACCCGGTAGGACGTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGCGCCAg               >  1:460343/1‑150 (MQ=255)
                                                                                                                                       gcgcATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAg               >  2:562932/1‑150 (MQ=255)
                                                                                                                                              ttGGCCGGCCAGTAGCTGCATTAGATGTAGCTTTGTGCGGGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAGCGAGGCg        <  2:307153/150‑1 (MQ=255)
                                                                                                                                                 gCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCAcagcag                           >  2:1330687/1‑128 (MQ=255)
                                                                                                                                                 gCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCAcagcag                           <  1:1330687/128‑1 (MQ=255)
                                                                                                                                                   cAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGc                                                                        >  2:1162346/1‑81 (MQ=255)
                                                                                                                                                   cAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGc                                                                        <  1:1162346/81‑1 (MQ=255)
                                                                                                                                                   cAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAGCGAGGCGGTGCt   <  1:209064/150‑1 (MQ=255)
                                                                                                                                                    aGCCTGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAGCGAGGCGGTGCTg  >  2:877700/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATGTCGTTGAGCAGTTCGTAGGTGCGGTTGCGCTGCTCCAGCAGGCGGCCCGGCGGCAGCACCAGGTCGTTGATGCTCTGGTAGCCGCCCAGCGCGGTCTGGATGGCATGCTGGGCCGGCACGTTGGCGCACAGGCGCATGTTGGCCAGCATGTCGATGCCTTCGATGTAGCTGTGTGCGTGGTGCTTGGGCCCGGAAATGATCAGCCAGCCAGAGCGGAAGCCCGCCACCCGGTACGACTTGGACAAGCCGTTGAAGGTCAGGCACAGCAGGTCCGGAGCCAGCGAGGCGGTGCTG  >  NC_002947/2095564‑2095861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑