Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,127,894 G→T 7.3% L1074F (TTG→TTT)  PP_1887 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,127,8940GT 7.3% 170.1 / 2.8 55L1074F (TTG→TTT) PP_1887hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

TGCACGCGCCTGAGCCCAAAAGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGAAGGGCAGCGAC  >  NC_002947/2127746‑2128041
                                                                                                                                                    |                                                                                                                                                   
tGCACGCGCCTGAGCCCAAAAGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTgg                                                                                                                                                    <  1:519062/150‑1 (MQ=255)
     gcgcCTGAGCCCAAAAGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGATTTGGCAAct                                                                                                                                               >  2:612573/1‑150 (MQ=255)
             gCCCAAAAGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGa                                                                                                                                       <  1:1352011/150‑1 (MQ=255)
                 aaaaGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCt                                                                                                                                          <  2:467944/143‑1 (MQ=255)
                 aaaaGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCt                                                                                                                                          >  1:467944/1‑143 (MQ=255)
                          cGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCATGAATGTATTTCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTc                                                                                                                          <  1:1242103/150‑1 (MQ=255)
                                 ggCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAggg                                                                                                                   <  2:1278262/150‑1 (MQ=255)
                                    tGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTg                                                                                                                                                     >  2:787011/1‑113 (MQ=255)
                                    tGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTg                                                                                                                                                     <  1:787011/113‑1 (MQ=255)
                                                cTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCt                                                                                                    <  1:915299/150‑1 (MQ=255)
                                                  gCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTc                                                                                                  <  1:160233/150‑1 (MQ=255)
                                                    cAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGa                                                                                                >  1:750033/1‑150 (MQ=255)
                                                    cAGGCAACTGCTGCTCCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGTCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGa                                                                                                <  2:1324172/150‑1 (MQ=255)
                                                     aGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGAt                                                                                               >  1:1089463/1‑150 (MQ=255)
                                                        cAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAgg                                                                                                                    >  2:1181031/1‑126 (MQ=255)
                                                        cAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAgg                                                                                                                    <  1:1181031/126‑1 (MQ=255)
                                                         aaCTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTg                                                                                           <  2:100549/150‑1 (MQ=255)
                                                         aaCTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGATTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTg                                                                                           <  1:612573/150‑1 (MQ=255)
                                                          aCTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTgg                                                                                          <  1:759456/150‑1 (MQ=255)
                                                           ctgctgctgctgGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGt                                                                                         <  1:1123003/150‑1 (MQ=255)
                                                               tgctgctgGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGc                                                                                                                                           <  1:568236/96‑1 (MQ=255)
                                                               tgctgctgGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGc                                                                                                                                           >  2:568236/1‑96 (MQ=255)
                                                                   gctgGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGCTGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACGGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGt                                                                                 >  2:407375/1‑150 (MQ=255)
                                                                    ctgGAGTCGACGTTCGGGTCTGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTg                                                                                <  2:937896/150‑1 (MQ=255)
                                                                       gAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTTGCAACTCTGCTCGAg                                                                                                                                      >  1:1405250/1‑93 (MQ=255)
                                                                       gAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTTGCAACTCTGCTCGAg                                                                                                                                      <  2:1405250/93‑1 (MQ=255)
                                                                       gAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTTGCAACTCTGCTCGAg                                                                                                                                      >  1:692361/1‑93 (MQ=255)
                                                                       gAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTTGCAACTCTGCTCGAg                                                                                                                                      <  2:692361/93‑1 (MQ=255)
                                                                            gACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGtt                                                                        >  2:1205946/1‑150 (MQ=255)
                                                                                   gggTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGAtttt                                                                                            >  2:978468/1‑123 (MQ=255)
                                                                                   gggTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGAtttt                                                                                            <  1:978468/123‑1 (MQ=255)
                                                                                    ggTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTg                                                                                           <  2:5903/123‑1 (MQ=255)
                                                                                    ggTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTg                                                                                           >  1:5903/1‑123 (MQ=255)
                                                                                     gTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGATGACACCGTGCAAACGTTGGCACAGGa                                                               >  1:220154/1‑150 (MQ=255)
                                                                                        tGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGAACAGGACTa                                                            >  2:689201/1‑150 (MQ=255)
                                                                                                  ggACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTc                                                  <  1:1406994/150‑1 (MQ=255)
                                                                                                   gACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCg                                                 >  1:212633/1‑150 (MQ=255)
                                                                                                             gTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGa                                                                                                >  2:79386/1‑93 (MQ=255)
                                                                                                             gTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGa                                                                                                <  1:79386/93‑1 (MQ=255)
                                                                                                             gTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGaa                                       <  1:47585/150‑1 (MQ=255)
                                                                                                             gTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGaa                                       <  1:610351/150‑1 (MQ=255)
                                                                                                              tCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAg                                      >  1:304222/1‑150 (MQ=255)
                                                                                                                   tACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGTACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCg                                 >  2:48639/1‑150 (MQ=255)
                                                                                                                      caccaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATc                                                                                                              >  1:1151668/1‑70 (MQ=255)
                                                                                                                      caccaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATc                                                                                                              <  2:1151668/70‑1 (MQ=255)
                                                                                                                      caccaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCAt                              >  1:1031207/1‑150 (MQ=255)
                                                                                                                       accaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAg                                                     >  2:136768/1‑126 (MQ=255)
                                                                                                                       accaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAg                                                     <  1:136768/126‑1 (MQ=255)
                                                                                                                       accaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGAtt                                              >  1:962312/1‑133 (MQ=255)
                                                                                                                       accaccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGAtt                                              <  2:962312/133‑1 (MQ=255)
                                                                                                                          accGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTg                          >  1:1338011/1‑150 (MQ=255)
                                                                                                                          accGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTg                          >  2:1105360/1‑150 (MQ=255)
                                                                                                                           ccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGa                         >  1:132675/1‑150 (MQ=255)
                                                                                                                           ccGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGa                         >  1:1357782/1‑150 (MQ=255)
                                                                                                                                       ccGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGa             >  2:1301123/1‑150 (MQ=255)
                                                                                                                                         gggCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGAAg           >  1:373035/1‑150 (MQ=255)
                                                                                                                                                  ttGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGAAGGGCAGcgac  <  1:1090487/150‑1 (MQ=255)
                                                                                                                                                  ttGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGAAGGGCAGcgac  <  2:1063334/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCACGCGCCTGAGCCCAAAAGGACTCGCTACCGGCTGCAGCGTGCGCCTGCCAGGCAACTGCTGCTGCTGGAGTCGACGTTCGGGTATGACCGCCAAGGACGCATGGCGTCCATTACCACCACCGATGCCAGCACCGGGCATGCTTTGGCAACTCTGCTCGAGTACGATGAATTCGACAGGGAGATCCTGCGGACCTTCGATTTTGGTGACACCGTGCAAACGTTGGCACAGGACTATGATGAGTTCGATTGTTTGAAGTCCCGCATTCTGAAAGAGCGCCCGAAGGGCAGCGAC  >  NC_002947/2127746‑2128041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑