Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,154,429 T→G 7.8% Q129P (CAG→CCG)  PP_1909 ← septum formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,154,4290TG 7.8% 145.9 / 4.8 51Q129P (CAG→CCG) PP_1909septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (20/27);  new base G (2/2);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

CTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTC  >  NC_002947/2154291‑2154574
                                                                                                                                          |                                                                                                                                                 
ctctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGccag                                                                                                                                        <  1:1221730/150‑3 (MQ=255)
 tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                       >  1:408657/1‑150 (MQ=255)
  ctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                      <  1:300314/150‑1 (MQ=255)
     gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgc                                                                                                                                   >  2:29044/1‑150 (MQ=255)
      aaCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCATGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgcg                                                                                                                                  <  2:1120847/150‑1 (MQ=255)
       aCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCCCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgcgc                                                                                                                                 <  1:328342/150‑1 (MQ=255)
                       ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                     >  1:269538/1‑130 (MQ=255)
                       ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                     <  2:269538/130‑1 (MQ=255)
                          cTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGccc                                                                                                              >  1:172707/1‑150 (MQ=255)
                           tCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCg                                                                                                             <  1:814518/150‑1 (MQ=255)
                                   gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                     >  1:10524/1‑150 (MQ=255)
                                     aaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGc                                                                                                   <  1:7886/150‑1 (MQ=255)
                                      aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCt                                                                                                  <  2:372318/150‑1 (MQ=255)
                                      aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCt                                                                                                  <  2:408657/150‑1 (MQ=255)
                                       cTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTc                                                                                                 >  1:68411/1‑150 (MQ=255)
                                           cTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                         >  1:251016/1‑138 (MQ=255)
                                           cTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                         <  2:251016/138‑1 (MQ=255)
                                                     cAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCt                                                                                   <  2:613346/150‑1 (MQ=255)
                                                         ggCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTgctgcacgctgcac                                                                               >  1:69198/1‑150 (MQ=255)
                                                          gCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGc                                                                                                                    >  2:456091/1‑112 (MQ=255)
                                                          gCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGc                                                                                                                    <  1:456091/112‑1 (MQ=255)
                                                           cTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACCACGATCCACTGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                             <  2:1107727/150‑1 (MQ=255)
                                                             gTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTTGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCa                                                                           <  2:904586/150‑1 (MQ=255)
                                                             gTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCa                                                                           <  2:172707/150‑1 (MQ=255)
                                                             gTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGATCTCAGCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCa                                                                           <  1:373799/150‑1 (MQ=255)
                                                                   ctgcCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGtt                                                                     <  1:578148/150‑1 (MQ=255)
                                                                               gCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                        >  1:714244/1‑87 (MQ=255)
                                                                               gCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                        <  2:714244/87‑1 (MQ=255)
                                                                                cTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgcg                                                        >  2:959381/1‑150 (MQ=255)
                                                                                                    gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagta                                                                        <  2:837914/114‑1 (MQ=255)
                                                                                                    gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagta                                                                        >  1:837914/1‑114 (MQ=255)
                                                                                                     ttCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                   >  1:142891/1‑150 (MQ=255)
                                                                                                      tCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGccc                                  <  1:29044/150‑1 (MQ=255)
                                                                                                                tCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                    >  1:569529/1‑74 (MQ=255)
                                                                                                                tCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                    <  2:569529/74‑1 (MQ=255)
                                                                                                                tCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCtt                                      <  1:768991/136‑1 (MQ=255)
                                                                                                                tCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCtt                                      >  2:768991/1‑136 (MQ=255)
                                                                                                                  aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCaa                                                             <  1:83090/111‑1 (MQ=255)
                                                                                                                  aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCaa                                                             >  2:83090/1‑111 (MQ=255)
                                                                                                                    ggTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGcc                    <  2:1393126/150‑1 (MQ=255)
                                                                                                                     gTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                           <  1:934543/110‑1 (MQ=255)
                                                                                                                     gTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                           >  2:934543/1‑110 (MQ=255)
                                                                                                                                gCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagta                                                                        >  1:1061734/1‑86 (MQ=255)
                                                                                                                                gCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagta                                                                        <  2:1061734/86‑1 (MQ=255)
                                                                                                                                  aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCGGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCt      >  1:1322806/1‑150 (MQ=255)
                                                                                                                                  aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                     <  1:486416/55‑1 (MQ=255)
                                                                                                                                  aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                     >  2:486416/1‑55 (MQ=255)
                                                                                                                                     ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGt   >  2:1340013/1‑150 (MQ=255)
                                                                                                                                      cACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCACGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTc  <  2:921807/150‑1 (MQ=255)
                                                                                                                                      cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCa                              <  2:827577/122‑1 (MQ=255)
                                                                                                                                      cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCa                              >  1:827577/1‑122 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
CTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTC  >  NC_002947/2154291‑2154574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑