Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,224,877 A→G 6.0% I600T (ATT→ACT)  PP_1963 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,224,8770AG 6.0% 199.1 / 2.4 66I600T (ATT→ACT) PP_1963hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base G (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

ATAGCGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTT  >  NC_002947/2224728‑2225023
                                                                                                                                                     |                                                                                                                                                  
aTAGCGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCaa                                                                                                                                                    <  2:43151/150‑1 (MQ=255)
      gggCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAg                                                                                                                                              >  2:654873/1‑150 (MQ=255)
        gCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATATTGAGTATGAGCt                                                                                                                                            <  1:238581/150‑1 (MQ=255)
          gTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAg                                                                                                                                          <  2:239965/150‑1 (MQ=255)
                      atatCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCaa                                                                                                                              >  2:1425644/1‑150 (MQ=255)
                       tatCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCaaa                                                                                                                             <  1:1220076/150‑1 (MQ=255)
                                  cTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCaa                                                                                                                                                    <  2:98176/116‑1 (MQ=255)
                                  cTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCaa                                                                                                                                                    >  1:98176/1‑116 (MQ=255)
                                   tGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAg                                                                                                                 >  2:1176026/1‑150 (MQ=255)
                                     cTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTc                                                                                                               <  1:654873/150‑1 (MQ=255)
                                           ggTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGCGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTAGTa                                                                                                         >  1:166057/1‑150 (MQ=255)
                                               tCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATa                                                                                                                           >  2:125810/1‑128 (MQ=255)
                                               tCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATa                                                                                                                           <  1:125810/128‑1 (MQ=255)
                                                    atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCaaa                                                                                                                             <  2:378607/121‑1 (MQ=255)
                                                    atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCaaa                                                                                                                             >  1:378607/1‑121 (MQ=255)
                                                    atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAgg                                                                                                                         <  2:1274933/125‑1 (MQ=255)
                                                    atcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAgg                                                                                                                         >  1:1274933/1‑125 (MQ=255)
                                                     tcatGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAAcc                                                                                               >  1:658149/1‑150 (MQ=255)
                                                        tGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCCgg                                                                                            <  1:1425644/150‑1 (MQ=255)
                                                          gatctCGTTGATGAGAAACTCCAAAATTTTACCTTCCATTCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                          <  1:1007676/146‑1 (MQ=255)
                                                          aTTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                          <  2:259457/150‑1 (MQ=255)
                                                                gTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAATTAGGGTAGGGAGGGTTCTTAAATGCGAACCTGGGagagata                                                                                    >  2:1007676/1‑145 (MQ=255)
                                                                gTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGcc                                                                                    >  1:1226499/1‑150 (MQ=255)
                                                                    atgaGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGaa                                                                                                 >  2:687197/1‑133 (MQ=255)
                                                                    atgaGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGaa                                                                                                 <  1:687197/133‑1 (MQ=255)
                                                                      gagaAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCa                                                                              >  2:536114/1‑150 (MQ=255)
                                                                        gaAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCAc                                                                             >  1:549857/1‑149 (MQ=255)
                                                                        gaAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCAc                                                                             <  2:549857/149‑1 (MQ=255)
                                                                             tCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                          >  1:1089537/1‑131 (MQ=255)
                                                                             tCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGa                                                                                          <  2:1089537/131‑1 (MQ=255)
                                                                               cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTcc                                                                                                                                   >  1:1077499/1‑88 (MQ=255)
                                                                               cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTcc                                                                                                                                   <  2:1077499/88‑1 (MQ=255)
                                                                               cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTAt                                                                     >  1:1014807/1‑150 (MQ=255)
                                                                               cAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTAt                                                                     >  2:322293/1‑150 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCaa                                                                                                                                                    >  2:882875/1‑70 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCaa                                                                                                                                                    <  1:882875/70‑1 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTc                                                                                                    <  2:1276133/118‑1 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTc                                                                                                    >  1:1276133/1‑118 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATg                                                                    >  2:980543/1‑150 (MQ=255)
                                                                                aaaaTTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACATTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGCGCAGCCGATGCACTTGTCTATg                                                                    >  1:25110/1‑150 (MQ=255)
                                                                                           ttCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCt                                                                  <  2:1095562/141‑1 (MQ=255)
                                                                                           ttCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATAAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCt                                                                  >  1:1095562/1‑141 (MQ=255)
                                                                                            tCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCAtttc                                                        <  1:536114/150‑1 (MQ=255)
                                                                                            tCCCAGCGCAGGTCAGAAGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTc                                                                                                               <  2:1262067/95‑1 (MQ=255)
                                                                                            tCCCAGCGCAGGTCAGAAGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTc                                                                                                               >  1:1262067/1‑95 (MQ=255)
                                                                                                   gcAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                    >  1:557797/1‑83 (MQ=255)
                                                                                                   gcAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGt                                                                                                                    <  2:557797/83‑1 (MQ=255)
                                                                                                    cAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGAt                                                                                 <  1:652189/117‑1 (MQ=255)
                                                                                                    cAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGAt                                                                                 >  2:652189/1‑117 (MQ=255)
                                                                                                     aGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTa                                               <  2:1226499/150‑1 (MQ=255)
                                                                                                     aGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTa                                               <  1:1176026/150‑1 (MQ=255)
                                                                                                     aGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTa                                               <  2:721107/150‑1 (MQ=255)
                                                                                                       gTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCAATTAgg                                             >  1:139895/1‑150 (MQ=255)
                                                                                                                  gTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGa                                                                                                                                               >  1:881035/1‑41 (MQ=255)
                                                                                                                  gTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGa                                                                                                                                               <  2:881035/41‑1 (MQ=255)
                                                                                                                  gTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCt                                  <  2:640568/150‑1 (MQ=255)
                                                                                                                   tGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTc                                 <  2:658149/150‑1 (MQ=255)
                                                                                                                        cTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTcc                                                   <  2:371174/127‑1 (MQ=255)
                                                                                                                        cTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTcc                                                   >  1:371174/1‑127 (MQ=255)
                                                                                                                              tttCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTc                                                                        <  1:457408/100‑1 (MQ=255)
                                                                                                                              tttCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTc                                                                        >  2:457408/1‑100 (MQ=255)
                                                                                                                                       ccAGCTCATACTCAATATGAGCTAGACGCGCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAATCTGGGAGTAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGtt             <  2:166057/150‑1 (MQ=255)
                                                                                                                                        taGCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTg            >  1:861606/2‑150 (MQ=255)
                                                                                                                                        cAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTg            >  1:586833/1‑150 (MQ=255)
                                                                                                                                         aGCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTc                                                                                                            <  1:107479/53‑1 (MQ=25)
                                                                                                                                         aGCTCATATTGAGTATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTc                                                                                                            >  2:107479/1‑53 (MQ=25)
                                                                                                                                                aCTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAt    <  1:980543/150‑1 (MQ=255)
                                                                                                                                                  tCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTc                  >  2:403055/1‑134 (MQ=255)
                                                                                                                                                  tCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTc                  <  1:403055/134‑1 (MQ=255)
                                                                                                                                                  tCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAttt  >  2:530139/1‑150 (MQ=255)
                                                                                                                                                  tCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGAttt  >  2:504232/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATAGCGGGGCGTTCCGCCGTCTATATCGCGAAGCCTGCTTGATGGTGTCTTCATCATGATTGTCGTTGATGAGAAACTCCAAAATTTTACCTTCCCAGCGCAGGTCAGAGGACCGTGCACCTTCGCTTTCATTTTCCAGCTCATACTCAATATGAGCTAGACGCTCCATCAAATAGGGTAGTGAGTCTTCGTAAATTCGAACCTGGGAGCAGCCGATGCACTTGTCTATGCTGTAGCATTTCTTTCCATTAGGAACCGATTGCTCGAATCCCTTCCAGTCTGGTTGATACTGATTT  >  NC_002947/2224728‑2225023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑