| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,256,242 | G→C | 14.0% | intergenic (+259/‑445) | asd → / → PP_1990 | aspartate‑semialdehyde dehydrogenase/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,256,242 | 0 | G | C | 14.0% | 111.7 / 14.7 | 50 | intergenic (+259/‑445) | asd/PP_1990 | aspartate‑semialdehyde dehydrogenase/transposase |
| Reads supporting (aligned to +/- strand): ref base G (22/21); new base C (4/3); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01 | |||||||||||
AATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCACGACAAAAAGG > NC_002947/2256099‑2256390 | aaTGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGccc < 2:1080940/150‑1 (MQ=255) tGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGt < 2:857659/150‑1 (MQ=255) gAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAg > 2:300148/1‑150 (MQ=255) tGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGc > 1:98888/1‑150 (MQ=255) ctGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCa < 2:645030/150‑1 (MQ=255) tGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAg > 1:512273/1‑150 (MQ=255) cAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGAt < 1:309944/150‑1 (MQ=255) agagCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGAGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATg > 2:1343670/1‑150 (MQ=255) cgttgccgttgccgttgcTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGcc < 1:1330887/150‑1 (MQ=255) gccgttgccgttttTTATGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCTGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCa < 2:747753/150‑1 (MQ=255) ccgttgcTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCACTGGGGCCCAGGATGGGGCCTGCAGCGCGGt > 1:1072281/1‑150 (MQ=255) gttgcTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGTGCCCCAGGATGGGGCCTGCAGCGCGGtcc > 2:335312/1‑150 (MQ=25) ttgcttttgcttttgcttCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGtcct > 2:977934/1‑150 (MQ=35) ttttgcttCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCa > 2:489463/1‑150 (MQ=32) gcttCTAAGTGCGCGGTAGTTCAGGCGCCGTAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGcc < 2:1143247/150‑1 (MQ=17) ttCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCg > 2:34791/1‑150 (MQ=32) tGCGCGGTAGTTCAGGCTACGTAGATTGCGACTTCAGGAGGCCGAGCTCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATCCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGCAGCAAGCCCGGAGCGAg < 2:366094/150‑1 (MQ=11) gcgGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCCTCAAGCGCTGTGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAgggg > 2:43360/1‑150 (MQ=17) gTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGAcc > 1:1126930/1‑150 (MQ=32) tAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGAccc < 1:977934/150‑1 (MQ=32) ggCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCg < 2:512273/150‑1 (MQ=32) cGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTa < 1:300148/150‑1 (MQ=32) gATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAgcgctca < 1:410360/150‑4 (MQ=18) cTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGgat > 1:54697/1‑150 (MQ=35) cAGGAGGCCGAGCCCAGGGCTTGCCGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGACCCCCCCAGCGCGGTCCTCCCGCCAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGgatgat < 1:675402/150‑1 (MQ=11) aggaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGatgatg < 2:1126930/150‑1 (MQ=35) ggaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATgg > 2:540820/1‑150 (MQ=35) cGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCg > 1:1322509/1‑150 (MQ=35) cAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGtt > 2:350336/1‑150 (MQ=35) cAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTACTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGtt > 1:601862/1‑150 (MQ=25) aGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGNTGGGCCCCAGGCTGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGttt > 1:1197607/1‑150 (MQ=25) gggttGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCAGGGCCCCAGGATGGGGCCTGCAGCGCGGGCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCAGGAGCGTAGCGCAGGGGCCGGATGATGGTAGCCGACGGttttt < 2:1020595/147‑1 (MQ=11) ggCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGttttt > 1:677439/1‑150 (MQ=35) gCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTg < 2:677439/150‑1 (MQ=37) gCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTc < 2:54697/150‑1 (MQ=40) gggggaggTGACGGGCATGGATGCCCGTGAAGCGCTGGGCTCCAGGATGGGGCCTGCAGTGCGGTGCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCtt < 1:1343670/148‑1 (MQ=255) agcgcttGACGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCtttt > 1:1348440/7‑150 (MQ=255) cttGACGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGatgatg > 2:577481/3‑120 (MQ=255) cttGACGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGatgatg < 1:577481/118‑1 (MQ=255) ttGACGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCa > 2:1199376/2‑150 (MQ=255) gTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAggg < 1:179606/108‑1 (MQ=33) gTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAggg > 2:179606/1‑108 (MQ=33) gACGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCACg > 2:991488/1‑150 (MQ=255) aCGGGCATCCATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCACGa < 1:1199376/150‑1 (MQ=255) gggCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGa > 2:1318731/1‑117 (MQ=38) gggCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGa < 1:1318731/117‑1 (MQ=37) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAgg < 1:979858/100‑1 (MQ=34) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAgg > 2:979858/1‑100 (MQ=34) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGatgatg > 2:781117/1‑112 (MQ=34) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGatgatg < 1:781117/112‑1 (MQ=34) ggATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCACGACAAAAAgg > 1:425555/1‑150 (MQ=255) | AATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCGACGGTTTTTGGTTCCTTTTTGCCACGACAAAAAGG > NC_002947/2256099‑2256390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |