Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,315,535 T→C 5.7% K154E (AAG→GAG)  PP_2037 ← aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,315,5350TC 5.7% 212.6 / 2.2 70K154E (AAG→GAG) PP_2037aldolase
Reads supporting (aligned to +/- strand):  ref base T (30/36);  new base C (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

ACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTGG  >  NC_002947/2315388‑2315683
                                                                                                                                                   |                                                                                                                                                    
aCAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCTTCCGTTGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGa                                                                                                                                                    <  1:374936/150‑1 (MQ=255)
 cagcagAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCGGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAg                                                                                                                                                   <  2:1276561/150‑1 (MQ=255)
     agagCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAAt                                                                                                                                               <  2:496404/150‑1 (MQ=255)
             cACGCAGGATTCCTCCATCGAACGCCCAGCGATCAGCAGGTCATGATGGGAAAGCAGGATTGCGCGCTTGTTGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCAACGGTACACCGGGCCATTCCTTGAGGAATGCACAgtc                                                                                                                                       <  2:35371/150‑1 (MQ=255)
                       tCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgg                                                                                                                             <  2:510815/150‑1 (MQ=255)
                       tCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgg                                                                                                                             <  1:540013/150‑1 (MQ=255)
                         cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCtt                                                                                                                                                      <  1:893803/123‑1 (MQ=255)
                         cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCtt                                                                                                                                                      >  2:893803/1‑123 (MQ=255)
                         cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgggg                                                                                                                           >  2:232104/1‑150 (MQ=255)
                         cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgggg                                                                                                                           <  1:232104/150‑1 (MQ=255)
                            gatcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggc                                                                                                                                            <  1:1256290/130‑3 (MQ=255)
                            gatcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggc                                                                                                                                            >  2:1256290/1‑128 (MQ=255)
                             atcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggccc                                                                                                                                          <  2:806111/131‑5 (MQ=255)
                             atcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggccc                                                                                                                                          >  1:806111/1‑127 (MQ=255)
                                gaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGca                                                                                                                                            <  1:1349734/126‑1 (MQ=255)
                                gaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGca                                                                                                                                            >  2:1349734/1‑126 (MQ=255)
                                     cccAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAt                                                                                                               <  1:396630/150‑1 (MQ=255)
                                      ccAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAtg                                                                                                              >  1:1263446/1‑150 (MQ=255)
                                        aGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAtgtg                                                                                                            >  1:1036043/1‑150 (MQ=255)
                                           gATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAt                                                                                                               <  2:1171329/144‑1 (MQ=255)
                                           gATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAt                                                                                                               >  1:1171329/1‑144 (MQ=255)
                                           gATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCga                                                                                                         <  1:228597/150‑1 (MQ=255)
                                            aTCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCgag                                                                                                        >  1:501136/1‑150 (MQ=255)
                                             tCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          >  1:218927/1‑131 (MQ=255)
                                             tCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          <  2:218927/131‑1 (MQ=255)
                                             tCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCgaga                                                                                                       <  1:1405342/150‑1 (MQ=255)
                                              cagcagGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGAt                                                                                                      <  2:1289208/150‑1 (MQ=255)
                                                gcagGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATgg                                                                                                    >  1:431279/1‑150 (MQ=255)
                                                gcagGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATgg                                                                                                    >  2:931199/1‑150 (MQ=255)
                                                 cagGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGc                                                                                                   >  2:216571/1‑150 (MQ=255)
                                                   ggCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGGGATGGCCa                                                                                                 <  2:237894/150‑1 (MQ=255)
                                                      cATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCg                                                                                              <  2:601143/150‑1 (MQ=255)
                                                      cATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCg                                                                                              >  1:608706/1‑150 (MQ=255)
                                                      cATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCg                                                                                              >  1:553931/1‑150 (MQ=255)
                                                        tgatgGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGa                                                                                                                                                    >  2:367374/1‑94 (MQ=255)
                                                        tgatgGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGa                                                                                                                                                    <  1:367374/94‑1 (MQ=255)
                                                              gAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAg                                                                                                                     >  1:791732/1‑119 (MQ=255)
                                                              gAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAg                                                                                                                     <  2:791732/119‑1 (MQ=255)
                                                               cgatctggATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGATATGGCCAGCGGAACTTCCa                                                                                     <  1:404212/144‑1 (MQ=255)
                                                                  gCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCAAAATCGGAAGAGAGTGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCa                                                                                  >  2:749355/1‑150 (MQ=255)
                                                                   cAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAAt                                                                                                                                               <  1:801363/88‑1 (MQ=255)
                                                                   cAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAAt                                                                                                                                               >  2:801363/1‑88 (MQ=255)
                                                                      gATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGa                                                                              <  1:931199/150‑1 (MQ=255)
                                                                               cTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAggcggc                                                                     >  1:1333753/1‑150 (MQ=255)
                                                                                     gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          >  1:1143728/1‑91 (MQ=255)
                                                                                     gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          <  2:1143728/91‑1 (MQ=255)
                                                                                     gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgg                                                                                                                             >  2:1308892/1‑88 (MQ=255)
                                                                                     gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgg                                                                                                                             <  1:1308892/88‑1 (MQ=255)
                                                                                     gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCTATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCtgtg                                                               >  2:1298059/1‑150 (MQ=255)
                                                                                          tCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCg                                                          >  2:1262833/1‑150 (MQ=255)
                                                                                               ttCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCgggtgg                                                     <  2:608706/150‑1 (MQ=255)
                                                                                                 cgcgATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATAGATAAGGCGGCGCTGTGCAGCgggtgggt                                                   <  1:970991/150‑1 (MQ=255)
                                                                                                    gatgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGtgtg                                                >  2:104423/1‑150 (MQ=255)
                                                                                                     atgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTgg                                               >  1:487499/1‑150 (MQ=255)
                                                                                                          ttCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGgatgat                                          <  2:431279/150‑1 (MQ=255)
                                                                                                            cGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGc                                        <  2:1211844/150‑1 (MQ=255)
                                                                                                              ccTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAg                                      <  1:688439/150‑1 (MQ=255)
                                                                                                               cttcttCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGt                                     >  1:193886/1‑150 (MQ=255)
                                                                                                                  cttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGa                                  <  2:501136/150‑1 (MQ=255)
                                                                                                                        tGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCATTTGACGTCgg                            <  1:344159/150‑1 (MQ=255)
                                                                                                                         gCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCggg                           >  1:770007/1‑150 (MQ=255)
                                                                                                                           ccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGaa                                                                                           >  2:946327/1‑84 (MQ=255)
                                                                                                                           ccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGaa                                                                                           <  1:946327/84‑1 (MQ=255)
                                                                                                                           ccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggc                         <  2:1237155/150‑1 (MQ=255)
                                                                                                                             aCCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGAACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggcgg                       >  2:559300/1‑150 (MQ=255)
                                                                                                                                     acCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGtc               >  2:324831/1‑149 (MQ=255)
                                                                                                                                       cGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAca             <  2:1263446/150‑1 (MQ=255)
                                                                                                                                       cGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGAACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAca             <  1:559300/150‑1 (MQ=255)
                                                                                                                                        gggCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAcag            >  2:58426/1‑150 (MQ=255)
                                                                                                                                          gCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCTATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAcagcc          <  1:1298059/150‑1 (MQ=255)
                                                                                                                                                 cTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCtgt    >  2:1191508/1‑149 (MQ=255)
                                                                                                                                                 cTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCtgt    <  1:1191508/149‑1 (MQ=255)
                                                                                                                                                  ttGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAGGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTgg  >  1:289144/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTGG  >  NC_002947/2315388‑2315683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑